Skip to main content
Erschienen in:

19.07.2022

Growth and reproduction of monoxenic and polyxenic cultures of the slug-parasitic nematodes Phasmarhabditis spp. in naturally occurring organic substrates

verfasst von: Jiří Nermuť, Miroslav Holley, Vladimír Půža

Erschienen in: BioControl | Ausgabe 5/2022

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

In the present study, we studied ecology of three species of mollusc-parasitic nematodes: Phasmarhabditis bohemica, P. bonaquaense and P. apuliae. We demonstrate that these facultative parasitic nematodes can grow in polyxenic and monoxenic combinations with bacteria Acinetobacter sp., Flavobacterium sp. and Pseudomonas sp. but some combinations do not support nematode reproduction. Bacteria Pseudomonas sp. did not support the growth of P. bonaquaense while P. apuliae grows only in combination with Flavobacterium sp. Composition of the culturing media apparently influenced the development time. Animal tissue-based substrates enabled slightly faster development than others. Similarly, also monoxenization of nematodes shortens the time to emergence of new dauer juveniles. Yields of dauer juveniles were usually higher in animal tissues than in other substrates. Generally, the most productive species was P. bohemica and the highest quality of dauer juveniles, expressed by their body size and lipid content, was reached in association with Flavobacterium sp. Our results provide the basis for further studies of monoxenic large scale production in liquid media and potentially development of these nematodes as promising mollusc biocontrol agents.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Literatur
Zurück zum Zitat Abu Hatab M, Gaugler R, Ehlers R-U (1998) Influence of culture method on Steinernema glaseri lipids. J Parasitol 84:215–221CrossRef Abu Hatab M, Gaugler R, Ehlers R-U (1998) Influence of culture method on Steinernema glaseri lipids. J Parasitol 84:215–221CrossRef
Zurück zum Zitat Abu Hatab M, Gaugler R (1999) Lipids of in vivo and in vitro cultured Heterorhabditis bacteriophora. Biol Control 15:133–188CrossRef Abu Hatab M, Gaugler R (1999) Lipids of in vivo and in vitro cultured Heterorhabditis bacteriophora. Biol Control 15:133–188CrossRef
Zurück zum Zitat Ehlers R-U, Shapiro Ilan DI (2005) Mass production. In: Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) Nematodes as biocontrol agents. CABI Publishing, Wallingford, pp 65–78CrossRef Ehlers R-U, Shapiro Ilan DI (2005) Mass production. In: Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) Nematodes as biocontrol agents. CABI Publishing, Wallingford, pp 65–78CrossRef
Zurück zum Zitat Friedman MJ (1990) Commercial production and development. In: Gaugler K, Kaya HK (eds) Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, pp 153–172 Friedman MJ (1990) Commercial production and development. In: Gaugler K, Kaya HK (eds) Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, pp 153–172
Zurück zum Zitat Hooper DJ, Wilson MJ, Rowe JA, Glen DM (1999) Some observations on the morphology and protein profiles of the slug-parasitic nematodes Phasmarhabditis hermaphrodita and P. neopapillosa (Nematoda: Rhabditidae). Nematology 1:173–182CrossRef Hooper DJ, Wilson MJ, Rowe JA, Glen DM (1999) Some observations on the morphology and protein profiles of the slug-parasitic nematodes Phasmarhabditis hermaphrodita and P. neopapillosa (Nematoda: Rhabditidae). Nematology 1:173–182CrossRef
Zurück zum Zitat Lunau S, Stoessel S, Schmidtpeisker AJ, Ehlers R-U (1993) Establishment of monoxenic inocula for scaling-up in vitro cultures of the entomopathogenic nematodes Steinernema spp. and Heterorhabditis spp. Nematologica 39:385–399CrossRef Lunau S, Stoessel S, Schmidtpeisker AJ, Ehlers R-U (1993) Establishment of monoxenic inocula for scaling-up in vitro cultures of the entomopathogenic nematodes Steinernema spp. and Heterorhabditis spp. Nematologica 39:385–399CrossRef
Zurück zum Zitat MacMillan K, Haukeland S, Rae R, Young I, Crawford J, Hapca S, Wilson MJ (2009) Dispersal patterns and behaviour of the nematode Phasmarhabditis hermaphrodita in mineral soils and organic media. Soil Biol Biochem 41:1483–1490CrossRef MacMillan K, Haukeland S, Rae R, Young I, Crawford J, Hapca S, Wilson MJ (2009) Dispersal patterns and behaviour of the nematode Phasmarhabditis hermaphrodita in mineral soils and organic media. Soil Biol Biochem 41:1483–1490CrossRef
Zurück zum Zitat Mengert H (1953) Nematoden und schnecken. Zeitschrif Für Morphologie Ökologie Tiere 48:311–349CrossRef Mengert H (1953) Nematoden und schnecken. Zeitschrif Für Morphologie Ökologie Tiere 48:311–349CrossRef
Zurück zum Zitat Nermuť J, Půža V, Mráček Z (2014) The effect of different growing substrates on the development and quality of Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae). Biocontrol Sci Technol 24:1026–1038CrossRef Nermuť J, Půža V, Mráček Z (2014) The effect of different growing substrates on the development and quality of Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae). Biocontrol Sci Technol 24:1026–1038CrossRef
Zurück zum Zitat Nermuť J, Půža V, Mekete T, Mráček Z (2016a) Phasmarhabditis bonaquaense n. sp (Nematoda: Rhabditidae), a new slug-parasitic nematode from the Czech Republic. Zootaxa 4179:530–546CrossRef Nermuť J, Půža V, Mekete T, Mráček Z (2016a) Phasmarhabditis bonaquaense n. sp (Nematoda: Rhabditidae), a new slug-parasitic nematode from the Czech Republic. Zootaxa 4179:530–546CrossRef
Zurück zum Zitat Nermuť J, Půža V, Mráček Z (2016b) Phasmarhabditis apuliae n. sp (Nematoda: Rhabditidae), a new rhabditid nematode from milacid slugs. Nematology 18:1095–1112CrossRef Nermuť J, Půža V, Mráček Z (2016b) Phasmarhabditis apuliae n. sp (Nematoda: Rhabditidae), a new rhabditid nematode from milacid slugs. Nematology 18:1095–1112CrossRef
Zurück zum Zitat Nermuť J, Půža V, Mekete T, Mráček Z (2017) Phasmarhabditis bohemica n. sp (Nematoda: Rhabditidae), a slug-parasitic nematode from the Czech Republic. Nematology 19:93–107CrossRef Nermuť J, Půža V, Mekete T, Mráček Z (2017) Phasmarhabditis bohemica n. sp (Nematoda: Rhabditidae), a slug-parasitic nematode from the Czech Republic. Nematology 19:93–107CrossRef
Zurück zum Zitat Nguyen KB, Smart GC (1995) Morphometrics of infective juveniles of Steinernema sp. and Heterorhabditis bacteriophora (Nematoda: Rhabditida). J Nematol 27:206–212PubMedPubMedCentral Nguyen KB, Smart GC (1995) Morphometrics of infective juveniles of Steinernema sp. and Heterorhabditis bacteriophora (Nematoda: Rhabditida). J Nematol 27:206–212PubMedPubMedCentral
Zurück zum Zitat Patel MN, Stolinski M, Wright DJ (1997) Neutral lipids and the assessment of infectivity in entomopathogenic nematodes: observations on four Steinernema species. Parasitology 114:489–496CrossRef Patel MN, Stolinski M, Wright DJ (1997) Neutral lipids and the assessment of infectivity in entomopathogenic nematodes: observations on four Steinernema species. Parasitology 114:489–496CrossRef
Zurück zum Zitat Pieterse, A. (2020) In vitro production and biocontrol potential of nematodes associated with molluscs. Doctoral thesis, Faculty of agriscience, University of Stellenbosch, Stellenbosch Pieterse, A. (2020) In vitro production and biocontrol potential of nematodes associated with molluscs. Doctoral thesis, Faculty of agriscience, University of Stellenbosch, Stellenbosch
Zurück zum Zitat Rae RG, Robertson JF, Wilson MJ (2006) The chemotactic response of Phasmarhabditis hermaphrodita (Nematoda: Rhabditida) to cues of Deroceras reticulatum (Mollusca: Gastropoda). Nematology 8:197–200CrossRef Rae RG, Robertson JF, Wilson MJ (2006) The chemotactic response of Phasmarhabditis hermaphrodita (Nematoda: Rhabditida) to cues of Deroceras reticulatum (Mollusca: Gastropoda). Nematology 8:197–200CrossRef
Zurück zum Zitat Rae R, Verdun C, Grewal P, Robertson JF, Wilson MJ (2007) Biological control of terrestrial molluscs using Phasmarhabditis hermaphrodita - progress and prospects. Pest Manag Sci 63:1153–1164CrossRef Rae R, Verdun C, Grewal P, Robertson JF, Wilson MJ (2007) Biological control of terrestrial molluscs using Phasmarhabditis hermaphrodita - progress and prospects. Pest Manag Sci 63:1153–1164CrossRef
Zurück zum Zitat Rae RG, Robertson JF, Wilson MJ (2009) Chemoattraction and host preference of the gastropod parasitic nematode Phasmarhabditis hermaphrodita. J Parasitol 95:517–526CrossRef Rae RG, Robertson JF, Wilson MJ (2009) Chemoattraction and host preference of the gastropod parasitic nematode Phasmarhabditis hermaphrodita. J Parasitol 95:517–526CrossRef
Zurück zum Zitat Rae RG, Tourna M, Wilson MJ (2010) The slug parasitic nematode Phasmarhabditis hermaphrodita associates with complex and variable bacterial assemblages that do not affect its virulence. J Invertebr Pathol 104:222–226CrossRef Rae RG, Tourna M, Wilson MJ (2010) The slug parasitic nematode Phasmarhabditis hermaphrodita associates with complex and variable bacterial assemblages that do not affect its virulence. J Invertebr Pathol 104:222–226CrossRef
Zurück zum Zitat Tan L, Grewal PS (2001) Infection behavior of the rhabditid nematode Phasmarhabditis hermaphrodita to the grey garden slug Deroceras reticulatum. J Parasitol 87:1349–1354CrossRef Tan L, Grewal PS (2001) Infection behavior of the rhabditid nematode Phasmarhabditis hermaphrodita to the grey garden slug Deroceras reticulatum. J Parasitol 87:1349–1354CrossRef
Zurück zum Zitat Wilson MJ, Glen DM, George SK, Pearce JD (1995a) Selection of a bacterium for the mass production of Phasmarhabditis hermaphrodita (Nematoda, Rhabditidae) as a biocontrol agent for slugs. Fundam Appl Nematol 18:419–425 Wilson MJ, Glen DM, George SK, Pearce JD (1995a) Selection of a bacterium for the mass production of Phasmarhabditis hermaphrodita (Nematoda, Rhabditidae) as a biocontrol agent for slugs. Fundam Appl Nematol 18:419–425
Zurück zum Zitat Wilson MJ, Glen DM, Pearce JD, Rodgers PB (1995b) Monoxenic culture of the slug parasite Phasmarhabditis hermaphrodita (Nematoda, Rhabditidae) with different bacteria in liquid and solid phase. Fundam Appl Nematol 18:159–166 Wilson MJ, Glen DM, Pearce JD, Rodgers PB (1995b) Monoxenic culture of the slug parasite Phasmarhabditis hermaphrodita (Nematoda, Rhabditidae) with different bacteria in liquid and solid phase. Fundam Appl Nematol 18:159–166
Zurück zum Zitat Wilson MJ (2012) Pathogens and parasites of terrestrial molluscs. In: Lacey LA (ed) Manual of techniques in invertebrate pathology. Academic Press, London, pp 427–439CrossRef Wilson MJ (2012) Pathogens and parasites of terrestrial molluscs. In: Lacey LA (ed) Manual of techniques in invertebrate pathology. Academic Press, London, pp 427–439CrossRef
Zurück zum Zitat Yang H, Jian H, Zhang S, Zhang G (1997) Quality of the entomopathogenic nematode Steinernema carpocapsae produced on different media. Biol Control 10:193–198CrossRef Yang H, Jian H, Zhang S, Zhang G (1997) Quality of the entomopathogenic nematode Steinernema carpocapsae produced on different media. Biol Control 10:193–198CrossRef
Metadaten
Titel
Growth and reproduction of monoxenic and polyxenic cultures of the slug-parasitic nematodes Phasmarhabditis spp. in naturally occurring organic substrates
verfasst von
Jiří Nermuť
Miroslav Holley
Vladimír Půža
Publikationsdatum
19.07.2022
Verlag
Springer Netherlands
Erschienen in
BioControl / Ausgabe 5/2022
Print ISSN: 1386-6141
Elektronische ISSN: 1573-8248
DOI
https://doi.org/10.1007/s10526-022-10152-8

Weitere Artikel der Ausgabe 5/2022

BioControl 5/2022 Zur Ausgabe