The reported Tri-Stepped Rectangular Monopole Antenna (TSRMA), for RF harvesting, can be further improvised in terms of performance. This can be achieved by optimizing the design parameters. In the current study, we report the design parameters which will help to create a better RF harvesting module. In the present manuscript, we show the effect of slot created on TSRMA in terms of gain. The TSRMA with and without slot are compared to show the differences. The effect of different types of rectifiers and the number of stages for harvester is also shown. It is found that Slotted TSRMA is better in performance than TSRMA. More than 50% improvement in the harvested energy is observed. Choice of rectifier and stages therein also found to be influencing the performance. Dickson rectifier has slight improvement (~ 8%) in the harvested energy over Villard rectifier. There is no significant improvement in 4–stage rectification over 2–stage rectification; we recommend 2–stage rectification in RF energy harvesters. This work focuses on the design and fabrication of an RF energy harvesting system which consists of an antenna having wide bandwidth (LTE850 (band 5), GSM900, GSM1800, 3G, 4G, and ISM 2.4 GHz) and high gain. The work also focuses on the comparative study of different rectifiers connected to a designed antenna to maximize the output DC energy. The harvested energy can be used for the charging of low-power devices. The work presented in the current manuscript can be directly applied to WSN sensor nodes used with smart watches for heart rate and blood pressure monitoring.