This paper presents a micro-biosensor based on Electrical Bio-Impedance Spectroscopy (EBIS), applied to blood cells diagnosis. Its hybrid conception uses the EBIS system coupled to a microfluidic device for cell by cell dynamic measurements. The microdevice is composed of 20x20
m² section micro channel, with platinum microelectrodes tested for different geometries. A Surface Acoustic Wave (SAW) device allows an accurate control of the fluidic displacement inside the channel. Simulation using athree dimensional Finite Elements Method (3D-FEM) have permitted to determinate the sensitivity of electrodes and to optimize their design, It also demonstrates the ability of our sensor to perform single cell measurements up to several hundred cells per second. First measurements confirms the simulation predictions and proved the ability to measure small impedance variations, less than 0.2%, during passage of particles.