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Erschienen in:
2011 | OriginalPaper | Buchkapitel
Identification of Wastewater Leaching into the Wells by HPLC-SEC Using UV and Fluorescence Detection
verfasst von : Hilda Marta Szabo, Tuula Tuhkanen
Erschienen in: Survival and Sustainability
Verlag: Springer Berlin Heidelberg
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Abstract
In this study, 36 small-scale wastewater purification systems effluents and 36 wells from sparsely populated and agricultural areas located in Pirkkanmma, Western Finland were analyzed during summer and fall 2006 by HPLC-SEC- (High Performance Liquid Size Exclusion Chromatography) coupled with on-line UV and fluorescence detector. In addition DOC (Dissolved Organic Carbon) of wastewater samples and wells nitrate concentration of the wells were determined. Two UV detection wavelengths, 224 and 254 nm respectively two excitation/emission wavelengths: 270 nm/355 nm (tryptophan-like fluorescence) and 270 nm/310 nm (tyrosine-like fluorescence) were used for detection. Wastewater leaching into the wells was not identified by tyrosine- and tryptophan-like fluorescence detection in whole-water samples. HPLC-SEC-UV at 224 and 254 nm was useful in indicating increased humic content and increased NO3, which could be caused by wastewater leaching.