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Erschienen in: Colloid and Polymer Science 7/2018

25.05.2018 | Original Contribution

Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC

verfasst von: Sergey K. Filippov, Aristeidis Papagiannopoulos, Anna Riabtseva, Stergios Pispas

Erschienen in: Colloid and Polymer Science | Ausgabe 7/2018

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Abstract

The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA block copolymer had a spherical shape and core-shell structure, which was confirmed by cryo-transmission electron microscopy (cryo-TEM) and SAXS. ITC revealed that lysozyme binds to the nanoparticle surface and that the strength of the binding depends on the ionic strength of the solution and the composition of the PnBA-b-PAA copolymers. Thermodynamic results unambiguously indicated that the interaction of lysozyme with the nanoparticle surface is a two-step process and entropy driven. Stopped-flow experiments proved that lysozyme penetration into the PAA corona occurs on a time scale of hundreds of milliseconds. Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.

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Metadaten
Titel
Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC
verfasst von
Sergey K. Filippov
Aristeidis Papagiannopoulos
Anna Riabtseva
Stergios Pispas
Publikationsdatum
25.05.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
Colloid and Polymer Science / Ausgabe 7/2018
Print ISSN: 0303-402X
Elektronische ISSN: 1435-1536
DOI
https://doi.org/10.1007/s00396-018-4329-4

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