Summary
Four different polymeric resins were tested as adsorbents in extractive bioconversion applied to the fermentative production of acetone and butanol by Clostridium acetobutylicum. The polymers were tested for their ability to adsorb butanol from pure solutions, and fermentation broths. Furthermore, the effect on the fermentability of the media was tested. The pH was increased to prevent adsorption of intermediates such as acetic and butyric acids. Bonopore, the polymer giving the best adsorption pattern with no undesirable effects, was tested in repeated batch cultures with C. acetobutylicum.
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References
Andrews GF, Elkcechen S (1984) Solid adsorbents in batch fermentations. Chem Eng. Commun 29:139–151
Ennis BM, Gutierrez NA, Maddox IS (1986) The acetone-butanol-ethanol fermentation: a current assessment. Process biochem 21:131–146
Groot WJ, Luyben KCAM (1986) In situ product recovery by adsorption in the butanol/isopropanol batch fermentation. Appl Microbiol Biotechnol 25:29–31
Häggström L (1985) Acetone-Butanol fermentations and its variants. Biotech Advs 3:13–28
Jones DT, Woods DR (1986) Acetone-Butanol fermentation revisited. Microbiol Rev 50:484–524
Larsson M, Holst O, Mattiasson B (1984) Butanol fermentation using a selective adsorbent for product recovery. In: Third European Congress on Biotechnology, vol 2, München, FRG, Sept 10–14, 1984, pp 313–316
Lee SS, Wang HY (1982) Repeated fed-batch rapid fermentation using yeast cells and activated carbon extraction system. Biotechnol Bioeng Symp 12:221–231
Lencki RW, Robinson CW, Moo-Young M (1983) On-line extraction of ethanol from fermentation broths using hydrophobic adsorbents. Biotechnol Bioeng Symp 13:617–628
Maddox IS (1982a) Production of ethanol and n-butanol from hexose/pentose mixtures using consecutive fermentations with Saccharomyces cerevisiae and Clostridium acetobutylicum. Biotechnol Lett 4:23–28
Maddox IS (1982b) Use of silicalite for the adsorption of n-butanol from fermentation liquors. Biotechnol Lett 4:759–760
Mattiasson B, Larsson M (1985) Extractive bioconversions with emphasis on solvent production. Biotechnol Genetic Eng Rev 3:137–174
Milestone NB, Bibby DM (1981) Concentration of alcohols by adsorption on silicalite. J Chem Tech Biotechnol 31:732–736
Moreira AR, Ulmer DC, Linden JC (1981) Butanol toxicity in the butylic fermentation. Biotech Bioeng Symp 11:567–579
Roffler SR, Blanch HW, Wilke CR (1984) In situ recovery of fermentation products. Trends Biotechnol 2:129–136
Roffler SR, Blanch HW, Wilke CR (1987) In-situ recovery of butanol during fermentations. Part 1: Batch extractive fermentations. Bioprocess Engineering 2:1–12
Wang HY (1983) Integrating biochemical separation and purification steps in fermentation processes. Ann NY Acad Sciences 413:313–321
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Nielsen, L., Larsson, M., Holst, O. et al. Adsorbents for extractive bioconversion applied to the acetone-butanol fermentation. Appl Microbiol Biotechnol 28, 335–339 (1988). https://doi.org/10.1007/BF00268191
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DOI: https://doi.org/10.1007/BF00268191