Abstract
Urease was entrap-immobilized on composite gel fibre of cellulose acetate and TiO2. The immobilization can be easily performed under mild conditions. The gel fibre is stable in high ionic solutions and in phosphate solutions with a range of pH 6–8.
The pH and thermal stabilities of immobilized urease were higher than those of the native one.
Similar content being viewed by others
References
I. Chibata (Ed.), Immobilized Bio-Catalysis (Kodansya Scientific, 1991), (in Japanese).
P.Gemeiner, V.Stefaca, and V.Bales, Enzyme Microb. Technol. 15, 551 (1993).
C.A.White and J.F.Kennedy, Enzyme Microb. Technol. 2, 82 (1980). H. Horizu, Jyokyo (j. Brewing Soc. Jpn.) 83, 524 (1988). (in Japanese).
Y.Kurokawa, T.Sano, H.Ohta and Y.Nakagawa, Biotech. & Bioeng. 42, 394 (1993). Y. Ohmori and Y. Kurokawa, J. Biotech. 33, 205 (1994).
M.Iida, Eiseikensa (Hygienic inspection) 38, 989 (1989).
H.J.Moynihan, C.K.Lee, W.Clark, and N.H.L.Wang, Biotech. & Bioeng. 34 951 (1989).
N.Kamath and S.F.D'Souza, Enzyme Microb. Technol. 13, 935 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hatayama, H., Swabe, T. & Kurokawa, Y. Immobilization of urease on composite fibre by using a gel formation of cellulose acetate and titanium iso-propoxide. J Sol-Gel Sci Technol 7, 13–17 (1996). https://doi.org/10.1007/BF00401878
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00401878