Abstract
A low frequency androgenesis was inducedin vitro in all experimental variants ofCapsicum anthers cultured in standard and minimalized media. The embryogenic frequency was stimulated by the presence of activated charcoal which also prolonged the vitality of the culture. The percentage of embryoids growing to complete plants increased, if the embryoids were removed from the anthers and cultured separately. The morphogenic initiation of globular embryoids was conditioned by the presence of FeEDTA in the culture medium. The stimulation of androgenesis initiation can be explained by the same change in the decisive factor caused by various effects. In long-term embryoid cultures, which stagnated in growing and seemingly necrotized, a second somatic embryogenesis occurred. About 10% of obtained androgenic plants were fertile and even gave progenies. The process can be recommended for breeding.
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Vagera, J., Havránek, P. In vitro induction of androgenesis inCapsicum annuum L. and its genetic aspects. Biol Plant 27, 10–21 (1985). https://doi.org/10.1007/BF02894626
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DOI: https://doi.org/10.1007/BF02894626