Skip to main content
SpringerLink
Log in

Glucoamylase production and nitrogen nutrition inAspergillus awamori

  • Session 3 Applied Biological Research II
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Studies related to glucoamylase (GA) production and nitrogen nutrition have been reported, and there has been a general agreement as to its importance, although no conclusions have emerged. The present investigation studies the physiological response of an industrial strain ofAspergillus awamori to variable nitrogen nutrition and C/N ratios on GA production. It relates to the use of amino and NH4+ nitrogen or a combination of both. Conditions have been identified that favor the enzyme production. The negative effect of NH4+ concentrations on products, such as antibiotics, pullulan, ligninases, and proteases, is reported in the literature. In some cases, nitrogen metabolite repression has been considered to explain the data obtained. This work discusses a possible role played by nitrogen metabolite repression in glucoamylase production. Batch experiments were carried out in an 8-L working volume fermenter.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Frost, G. M. and Moss, D. A. (1987), inBiotechnology, Rehm, H.-J. and Reed, G., eds., vol. 7a,Enzyme Technology, Kennedy, J. F., ed., VCH Publishers, Ltd., Cambridge, p. 129.

    Google Scholar 

  2. Fogarty, W. M. (1983), inMicrobial Enzymes and Biotechnology, Fogarty, W. M., ed., Applied Science Publishers Ltd., London, p. 22.

    Google Scholar 

  3. Galliard, T. (1987), inStarch: Properties and Potential, Galliard, T., ed., John Wiley & Sons, Chichester, p. 1.

    Google Scholar 

  4. Mercier, C. and Colonna, P. (1988), Starch and enzymes: innovations in the products processes and uses.VIII Symposium International de Biotechnologie-Abstract of communication, Paris, 17th to 22nd of July.

  5. Halpern, M. G. (1981),Industrial Enzymes for Microbial Sources, Noyes Data Corporation, Park Ridge, NJ, p. 234.

    Google Scholar 

  6. Alazard, D. and Raimbaut, M. (1981),Eur. J. Appl. Microbiol. Biotechnol. 12, 133.

    Article  Google Scholar 

  7. Andrzejczuk-Hybel, J., Smiley, K. L., and Kaczkoswki, J. (1985),Acta Aliment. Pol. 11(3), 333.

    CAS  Google Scholar 

  8. Attia, R. M. and Ali, S. A. (1977),Zentralbl. Bakteriol. Parasitenkd., Infektions Kr. Hyg., Abt 2 132(4), 322.

    CAS  Google Scholar 

  9. Feniksova, R. V. and Ryzhakova, V. G. (1968),Prikl. Biokhim. Mikrobiol. 4(2), 124.

    Google Scholar 

  10. Feniksova, R. V. and Ryzhakova, V. G. (1971),Chem. Abstracts 74, 86373m.

    Google Scholar 

  11. Hayashida, S. (1975),Agric. Biol. Chem. 39(11), 2093.

    CAS  Google Scholar 

  12. Hayashida, S. and Flor, P. Q. (1982),Kawachi. Agric. Biol. Chem. 46(6), 1639.

    CAS  Google Scholar 

  13. Hayashida, S., Nakahara, K., Kuroda, K., Kamachi, T., Ohta, K., Iwanaga, S., Miyata, T., and Sakaki, Y. (1988),Agric. Biol. Chem. 52(1), 273.

    CAS  Google Scholar 

  14. Hayashida, S. and Yoshino, E. (1978),Agric. Biol. Chem. 42(5), 927.

    CAS  Google Scholar 

  15. Lineback, D. R., Georgi, C. E., and Doty, R. L. (1966),J. Gen. Appl. Microbiol. 12(1), 27.

    Article  CAS  Google Scholar 

  16. Lineback, D. R. and Aira, L. A. (1972),Cereal Chemistry 49, 283.

    CAS  Google Scholar 

  17. Lineback, D. R., Russel, I. J., and Rasmussem, C. (1969),Arch. Biochem. Biophys. 143, 539.

    Article  Google Scholar 

  18. Pestana, F. and Castillo, F. J. (1985),MIRCEN J. 1, 225.

    Article  CAS  Google Scholar 

  19. Barton, L. L., Georgi, C. E., and Lineback, D. R. (1972),J. Bacteriol. 111(3), 771.

    CAS  Google Scholar 

  20. Barton, L. L., Lineback, D. R., Georgi, C. E. (1969),J. Gen. Appl. Microbiol. 15, 327.

    Article  CAS  Google Scholar 

  21. Bhella, R. S. and Altosaar, I. (1988),Curr. Genet. 14(3), 247.

    Article  CAS  Google Scholar 

  22. Feniksova, R. V. and Musaeva, T. I. (1968),Chem. Abstracts 68, 10557e.

    Google Scholar 

  23. Attia, R. M. and Ali, S. A. (1974),Agric. Res. Rev. 52(4), 131.

    CAS  Google Scholar 

  24. Qadeer, M. A. and Kausar, T. (1971),Pak. J. Sci. Ind. Res. 14(3), 247.

    CAS  Google Scholar 

  25. Sreekantiah, K. R., Jaleel, S. A., and Rao, T. N. R. (1973),Chem. Mikrobiol. Technol. Lebensm. 2(2), 42.

    CAS  Google Scholar 

  26. Cohen, B. L. (1972),J. Gen. Microbiol. 71, 293.

    CAS  Google Scholar 

  27. Cohen, B. L. and Drucker, H. (1977),Arch. Biochem. Biophys. 182, 601.

    Article  CAS  Google Scholar 

  28. Drucker, H. (1972),J. Bacteriol. 110(3), 1041.

    CAS  Google Scholar 

  29. Drucker, H. (1973),J. Bacteriol. 116(2), 593.

    CAS  Google Scholar 

  30. Flores, E. M. and Sanchez, S. (1985),FEMS Microbiol. Lett. 26, 191.

    Article  CAS  Google Scholar 

  31. Gräfe, U., Bocker, H., Reinhardt, G., Tkocz, H., and Thrum, H. (1979),L. Allg. Mikrobiol. 14, 181.

    Article  Google Scholar 

  32. Inoue, S., Nishizawa, Y., and Nagai, S. (1983),J. Ferment. Technol. 6(1), 7.

    Google Scholar 

  33. Karatsu, Y., Sakurai, M., Inuzuka, K., and Suzuki, T. (1982),J. Ferment. Technol. 60(6), 603.

    Google Scholar 

  34. Karatsu, Y., Sakurai, M., Inuzuka, K., and Suzuki, T. (1983),J. Ferment. Technol. 61(4), 365.

    Google Scholar 

  35. Karatsu, Y., Arai, Y., Inuzuka, K., and Suzuki, T. (1983),Agric. Biol. Chem. 47(11), 2607.

    Google Scholar 

  36. Karatsu, Y. and Inuzuka, K. (1984),Agric. Biol. Chem. 48(9), 2389.

    Google Scholar 

  37. Martin, J. F. and Demain, A. L. (1980),Microbiol. Rev. 44(2), 230.

    CAS  Google Scholar 

  38. Masuma, R., Tanaka, Y., and Ömura, S. (1983),J. Ferment. Technol. 61(6), 607.

    CAS  Google Scholar 

  39. Shapiro, S. and Vining, L. C. (1983),Can. J. Microbiol. 29, 1706.

    CAS  Google Scholar 

  40. Shapiro, S. and Vining, L. C. (1984),Can. J. Microbiol. 30, 798.

    Article  CAS  Google Scholar 

  41. Suzuki, T., Yamane, T., and Shimizu, S. (1988),Appl. Microbiol. Biotechnol. 28, 188.

    Article  CAS  Google Scholar 

  42. Shapiro, S. and Vining, L. C. (1985),Can. J. Microbiol. 31, 119.

    CAS  Google Scholar 

  43. Vu-Trong, K. and Gray, P. P. (1987),Enzyme Microb. Technol. 9, 590.

    Article  CAS  Google Scholar 

  44. Fenn, P., Choi, S., and Kirk, T. K. (1981),Arch. Microbiol. 130, 66.

    Article  CAS  Google Scholar 

  45. Keyser, P., Kirk, T. K., and Zeikus, J. G. (1978),J. Bacteriol. 135(4), 790.

    CAS  Google Scholar 

  46. Kirk, T. K. and Fenn, P. (1981),Arch. Microbiol. 130, 59.

    Article  Google Scholar 

  47. Kirk, T. K., Schultz, E., Connors, W. J., Lorenz, L. F., and Zeikus, J. G. (1978),Arch. Microbiol. 117, 277.

    Article  CAS  Google Scholar 

  48. Reid, I. D. (1979),Can. J. Bot. 57, 2050.

    Article  CAS  Google Scholar 

  49. Reid, I. D. (1983),Appl. Environ. Microbiol. 45, 830.

    CAS  Google Scholar 

  50. Reid, I. D. (1983),Appl. Environ. Microbiol. 45, 838.

    CAS  Google Scholar 

  51. Bulmer, M. A., Catley, B. J., and Kelly, P. J. (1987),Appl. Microbiol. Biotechnol. 25, 362.

    Article  CAS  Google Scholar 

  52. Seviour, R. J. and Kristiansen, B. (1983),Eur. J. Appl. Microbiol. Biotechnol 17, 178.

    Article  CAS  Google Scholar 

  53. Arst, H. N., Jr. and Cove, D. J. (1973),Molec. Gen. Genet. 126, 111.

    Article  CAS  Google Scholar 

  54. Cook, R. J. and Anthony, C. (1978),J. Gen. Microbiol. 109, 265.

    CAS  Google Scholar 

  55. Cook, R. J. and Anthony, C. (1978),J. Gen. Microbiol. 109, 275.

    CAS  Google Scholar 

  56. Dunn-Coleman, N. S. and Garrett, R. H. (1980),Molec. Gen. Genet. 179, 25.

    Article  CAS  Google Scholar 

  57. Hackette, S. L., Skye, G. E., Burton, C., and Segel, I. H. (1970),The J. Biological Chem. 247(17), 4241.

    Google Scholar 

  58. Hunter, D. R. and Segel, I. H. (1971),Arch. Biochem. Biophys. 144, 168.

    Article  CAS  Google Scholar 

  59. Hynes, M. J. (1970),J. Bacteriol. 103, 482.

    CAS  Google Scholar 

  60. Hynes, M. J. (1974),J. Bacteriol. 120(3), 1116.

    CAS  Google Scholar 

  61. Marzluf, G. A. (1981),Microbiol. Rev. 45(3), 437.

    CAS  Google Scholar 

  62. Morton, A. G. and Macmillan, A. (1953),J. Experi. Bot. 5(14), 232.

    Google Scholar 

  63. Pateman, J. A., Kinghorn, J. R., Dunn, E., and Forbes, E. (1973),J. Bacteriol. 114(3), 943.

    CAS  Google Scholar 

  64. Robinson, J. H., Anthony, C., and Drabble, W. T. (1973),J. Gen. Microbiol. 79, 53.

    CAS  Google Scholar 

  65. Arst, H. N., Jr. (1981), inGenetics as a Tool in Microbiology, Glover, S. W. and Hopwood, D. A., eds., Cambridge University Press, Cambridge, p. 131.

    Google Scholar 

  66. Arst, H. N., Jr. and Scazzocchio, C. (1985), inGene Manipulations in Fungi, Bennet, J. K. and Lasure, L. L., eds., Academic, New York, p. 309.

    Google Scholar 

  67. Priest, F. G. (1983), inMicrobial Enzymes and Biotechnology, Fogarty, W. M., ed., Applied Science Publishers Ltd., London, p. 319.

    Google Scholar 

  68. Wiame, J.-M., Grenson, M., and Arst, H. N., Jr. (1985), inAdvances in Microbial Physiology, vol. 26, Rose, A. H. and Tempest, D. W., eds., Academic, London, p. 2.

    Google Scholar 

  69. Darnel, J., Lodish, H., and Baltimore, D. (1990), inMolecular Cell Biology, Scientific American Books, W. H. Freeman and Company, New York, p. 229.

    Google Scholar 

  70. Tyler, B. (1978),Ann. Rev. Biochem. 47, 1127.

    Article  CAS  Google Scholar 

  71. Magasanik, B., Brival, M. J., Brenchliy, J. E., Tyler, B. M., De Leo, A. B., Streicher, S. L., Bender, R. A., and Paris, C. G. (1974), inCurrent Topics in Cellular Regulation, vol. 8, Horecker, B. L. and Stadtman, E. R., eds., Academic, New York, p. 119.

    Google Scholar 

  72. Onion, A. H. S. (1971), inMethods in Microbiology, vol. 4, Booth, C., ed., Academic, London, p. 113.

    Google Scholar 

  73. Perkins, D. D. (1962),Can. J. Microbiol. 8, 591.

    Article  Google Scholar 

  74. Trollope, D. R. (1975),J. Appl. Bacteriol. 38, 115.

    CAS  Google Scholar 

  75. Bon, E. P. S. (1991), Glycoamylase production and nitrogen metabolism inAspergillus awamori, PhD. thesis, Department of Chem. Engineering, UMIST, Manchester, UK.

    Google Scholar 

  76. Korshunov, V. V. and Loginova, L. G. (1988),Prikl. Biokhim. Mikrobiol. 24, 67.

    Google Scholar 

  77. Bon, E. and Webb, C. (1989),Enzyme Microb. Technol. 11, 495.

    Article  CAS  Google Scholar 

  78. Ainsworth, G. C. and Sussman, A. S. eds. (1965),The Fungi, Vol. 1: The Fungal Cell, Academic, London.

    Google Scholar 

  79. Michelena, V. V. and Castillo, F. J. (1984),J. Appl. Bacteriol. 56, 395.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CT-Bl.A, Inst. de Química-UFRJ, Ilha do Fundão, CEP 21945, Rio de Janeiro, RJ, Brazil

    E. Bon

  2. Department of Chem. Engineering, UMIST, PO Box 88, M60 1QD, Manchester, UK

    C. Webb

Authors
  1. E. Bon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Webb
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bon, E., Webb, C. Glucoamylase production and nitrogen nutrition inAspergillus awamori . Appl Biochem Biotechnol 39, 349–369 (1993). https://doi.org/10.1007/BF02919002

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02919002

Index Entries

Search

Navigation