Abstract
Understanding the role of colloids in the environment needs a more complete characterization of their size and shape than the usually used hydrodynamic diameter. A novel methodology to determine three dimensional description of colloids has been successfully experimented in this work by coupling Flow-Field Flow Fractionation (FlFFF) to Multi Angle Laser Light Scattering (MALLS), and Transmission Electron Microscope (TEM). This methodology was evaluated for a soil colloidal sample. Results show that, the particles surface areas determined by FlFFF-MALLS-TEM, are 3–4 times greater than those calculated using the hydrodynamic diameter determined by FlFFF. Further, results indicated that, the surface area/volume values are 3–5 times greater than those calculated based on the hydrodynamic diameter. Therefore, a correction factor must be applied when the particle surface area is calculated from the hydrodynamic diameter determined by FlFFF.
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Baalousha, M., Kammer, F.V.D., Motelica-Heino, M. et al. 3D characterization of natural colloids by FlFFF-MALLS-TEM. Anal Bioanal Chem 383, 549–556 (2005). https://doi.org/10.1007/s00216-005-0006-9
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DOI: https://doi.org/10.1007/s00216-005-0006-9