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Amperometric nitrite sensor based on hemoglobin/colloidal gold nanoparticles immobilized on a glassy carbon electrode by a titania sol-gel film

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Abstract

A novel amperometric nitrite sensor was developed based on the immobilization of hemoglobin/colloidal gold nanoparticles on a glassy carbon electrode by a titania sol-gel film. The sensor shows a pair of well-defined and nearly reversible cyclic voltammogram peaks for Hb Fe(III)/Fe(II) with a formal potential (E°′) of −0.370 V, and the peak-to-peak separation at 100 mV s−1 was 66 mV vs. Ag/AgCl (3.0 M KCl) in a pH 6.9 phosphate buffer solution. The formal potential of the Hb Fe(III)/Fe(II) couple shifted linearly with pH with a slope of −50.0 mV/pH, indicating that electron transfer accompanies single-proton transportation. The sensor exhibited an excellent electrocatalytic response to the reduction of nitrite. The reduction overpotential was 0.45 V below that obtained at a colloidal gold nanoparticles/TiO2 sol-gel film-modified GCE. The linear range for nitrite determination for the sensor was 4.0×10−6 to 3.5×10−4 M, with a detection limit of 1.2×10−6 M. The stability, repeatability and selectivity of the sensor were also evaluated.

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Acknowledgements

This work was supported by the Major State Basic Research Development Program (G2000078102), Educational Ministry (20020183007), National Natural Science Foundation (20175007) of China, and Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University.

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Authors and Affiliations

  1. College of Chemistry, Jilin University, Changchun, 130023, People’s Republic of China

    Weiwei Yang, Yu Bai, Yancai Li & Changqing Sun

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  1. Weiwei Yang
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Correspondence to Changqing Sun.

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Yang, W., Bai, Y., Li, Y. et al. Amperometric nitrite sensor based on hemoglobin/colloidal gold nanoparticles immobilized on a glassy carbon electrode by a titania sol-gel film. Anal Bioanal Chem 382, 44–50 (2005). https://doi.org/10.1007/s00216-005-3160-1

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