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Aminated dendritic surfaces characterization: a rapid and versatile colorimetric assay for estimating the amine density and coating stability

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Abstract

The functionalization of surfaces with amino groups is used in many application areas such as in industrial biocatalytic processes for the development of medical biomaterials and in the environment for removing pollutants from water. Amino group density and grafting stability are often related to functionalized material performances; thus, their characterizations are of prime importance. The determination of amino density and grafting stability on polymeric material (e.g. polypropylene, polystyrene and cylco olefin copolymer) is often time consuming and sometimes presents technical constraints, more particularly with non-flat materials. In this paper, we report a novel colorimetric assay using the Coomassie Brilliant Blue dye for both amino density determination and grafting stability measurement. The assay named ADECA for “Amino Density Estimation by Colorimetric Assay” is sensitive, rapid, robust and versatile. We demonstrate that ADECA makes the evaluation of aminated materials performances possible for numerous material compositions, formats and chemistries used for grafting. Our study focuses on dendrigraft of poly-l-lysine and poly(amidoamine) dendrimers dendritic materials.

Animated surfaces characterization

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Acknowledgments

We thank the French National Space Agency (CNES) for financial grant (convention number 05/2182/00-DCT094).

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Authors and Affiliations

  1. Institut des Biomolécules Max Mousseron, CNRS, Université Montpellier I and II, UMR 5247, CC 1706, Place Bataillon, 34095, Montpellier Cedex, France

    G. Coussot, C. Faye, A. Ibrahim & O. Vandenabeele-Trambouze

  2. Laboratoire de Microscopie en Champ Proche (LMCP), Centre de Technologie de Montpellier, Université Montpellier II, CC 082, Place Bataillon, 34095, Montpellier Cedex, France

    M. Ramonda

  3. Laboratoire d’Astrophysique de Bordeaux (LAB), Université de Bordeaux, 2, rue de l’Observatoire, BP 89, 33271, Floirac Cedex, France

    M. Dobrijevic & A. Le Postollec

  4. CNRS/INSU, UMR 5804, 33271, Floirac, France

    M. Dobrijevic & A. Le Postollec

  5. COLCOM, Cap-Alpha, Av. de l’Europe, Clapiers, 34940, Montpellier Cedex 9, France

    F. Granier

Authors
  1. G. Coussot
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  2. C. Faye
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  3. A. Ibrahim
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  4. M. Ramonda
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  5. M. Dobrijevic
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  6. A. Le Postollec
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  7. F. Granier
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  8. O. Vandenabeele-Trambouze
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Corresponding author

Correspondence to O. Vandenabeele-Trambouze.

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Coussot, G., Faye, C., Ibrahim, A. et al. Aminated dendritic surfaces characterization: a rapid and versatile colorimetric assay for estimating the amine density and coating stability. Anal Bioanal Chem 399, 2295–2302 (2011). https://doi.org/10.1007/s00216-010-4612-9

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  • DOI: https://doi.org/10.1007/s00216-010-4612-9

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