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Surface-enhanced vibrational spectroscopy of B vitamins: what is the effect of SERS-active metals used?

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Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy and surface-enhanced infrared absorption (SEIRA) spectroscopy are analytical tools suitable for the detection of small amounts of various analytes adsorbed on metal surfaces. During recent years, these two spectroscopic methods have become increasingly important in the investigation of adsorption of biomolecules and pharmaceuticals on nanostructured metal surfaces. In this work, the adsorption of B-group vitamins pyridoxine, nicotinic acid, folic acid and riboflavin at electrochemically prepared gold and silver substrates was investigated using Fourier transform SERS spectroscopy at an excitation wavelength of 1,064 nm. Gold and silver substrates were prepared by cathodic reduction on massive platinum targets. In the case of gold substrates, oxidation–reduction cycles were applied to increase the enhancement factor of the gold surface. The SERS spectra of riboflavin, nicotinic acid, folic acid and pyridoxine adsorbed on silver substrates differ significantly from SERS spectra of these B-group vitamins adsorbed on gold substrates. The analysis of near-infrared-excited SERS spectra reveals that each of B-group vitamin investigated interacts with the gold surface via a different mechanism of adsorption to that with the silver surface. In the case of riboflavin adsorbed on silver substrate, the interpretation of surface-enhanced infrared absorption (SEIRA) spectra was also helpful in investigation of the adsorption mechanism.

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References

  1. Fleishmann M, Hendra PJ, McQuillan AJ (1974) Chem Phys Lett 26:163–166

    Article  Google Scholar 

  2. Moskovits M (2005) J Raman Spectrosc 36:485–496

    Article  CAS  Google Scholar 

  3. Campion A, Kambhampati P (2005) Chem Soc Rev 27:241–250

    Article  Google Scholar 

  4. Osawa M, Ataka K-I, Yoshii K, Nishikawa Y (1993) Appl Spectrosc 47:1497–1502

    Article  CAS  Google Scholar 

  5. Delgado JM, Orts JM, Pérez JM, Rodes A (2008) J Electroanal Chem 617: 130 140

    Google Scholar 

  6. Sutherland WS, Laserna JJ, Angebranndt MJ, Winefordner JD (1990) Anal Chem 62:689–693

    Article  CAS  Google Scholar 

  7. Torres EL, Winefordner JD (1987) Anal Chem 59:1626–1632

    Article  CAS  Google Scholar 

  8. Rupérez A, Montes R, Laserna JJ (1991) Vib Spectrosc 2:145–154

    Article  Google Scholar 

  9. Calvo N, Montes R, Laserna JJ (1993) Anal Chim Acta 280:263–268

    Article  CAS  Google Scholar 

  10. Fabriciova G, Sanchez-Cortes S, Garcia-Ramos JV, Miskovsky P (2004) J Raman Spectrosc 35:384–389

    Article  CAS  Google Scholar 

  11. Fabriciova G, García-Ramos JV, Miskovsky P, Sanchez-Cortes S (2004) Vib Spectrosc 34:273–281

    Article  CAS  Google Scholar 

  12. Farquharson S, Shende C, Inscore FE, Maksymiuk P, Gift A (2005) J Raman Spectrosc 36:208–212

    Article  CAS  Google Scholar 

  13. Farquharson S, Gift AD, Shende C, Maksymiuk P, Inscore FE, Murran J (2005) Vib Spectrosc 38:79–84

    Article  CAS  Google Scholar 

  14. Smith M, Stambaugh K, Simth L, Son H-J, Gardner A, Cordova S, Posey K, Perry D, Biris AS (2009) Vib Spectrosc 49:288–297

    Article  CAS  Google Scholar 

  15. Moskovits M (1982) J Chem Phys 77:4408–4416

    Article  CAS  Google Scholar 

  16. Moskovits M, Suh JS (1984) J Phys Chem 88:5526–5530

    Article  CAS  Google Scholar 

  17. Hallmark VM, Campion A (1986) J Chem Phys 84:2933–2941

    Article  CAS  Google Scholar 

  18. Lucock M (2000) Mol Genet Metab 71:121

    Article  CAS  Google Scholar 

  19. Zubay GL, Parson WW, Vance DE (1995) In: Principles of Biochemistry (ed) Vitamins and coenzymes. Wm. C. Brown Comunications, Dubuque

  20. Wen R, Fang Y (2005) Vib Spectrosc 39:106–113

    Article  CAS  Google Scholar 

  21. Chattopadhyay S, Brahma SK (1993) Spectrochim Acta 49A:589–597

    CAS  Google Scholar 

  22. Dressler DH, Mastay Y, Rosenbluh M, Fleger Y (2009) J Mol Struct 935:92–96

    Article  CAS  Google Scholar 

  23. Wang Li-Ran, Fang Y (2006) Spectrochim Acta 63A:614–618

    CAS  Google Scholar 

  24. Soo Min Park, Kwan Kim, Myung Soo Kim (1995) J Mol Struct 344:195–203

    Article  Google Scholar 

  25. Imai Y, Kurokawa Y, Hara M, Fukushima M (1997) Spectrochim Acta 53A:1697–1700

    CAS  Google Scholar 

  26. Yoo H-S, Lee N-S, Hanazaki I (1992) J Raman Spectrosc 23:239–241

    Article  CAS  Google Scholar 

  27. Liang EJ, Gottges D, Kiefer W (1994) Appl Spectrosc 48:1088–1094

    Article  CAS  Google Scholar 

  28. Cinta S, Morari C, Vogel E, Maniu D, Aluas M, Iliescu T, Cozar O, Kiefer W (1999) Vib Spectrosc 19:329–334

    Article  CAS  Google Scholar 

  29. Chu Y, Che S, Zheng J, Li Z (2009) J Raman Spectrosc 40:229–233

    Article  CAS  Google Scholar 

  30. De Jesús MA, Giesfeldt KS, Sepaniak MJ (2003) Appl Spectrosc 57:428–438

    Article  Google Scholar 

  31. Stokes RJ, McBride E, Wilson CG, Girkin JM, Smith WE, Graham D (2008) Appl Spectrosc 62:371–376

    Article  CAS  Google Scholar 

  32. Prokopec V, Čejková J, Matějka P, Hasal P (2008) Surf Intefarce Anal 40:601–607

    Article  CAS  Google Scholar 

  33. Zaruba K, Matejka P, Volf R, Volka K, Kral V, Sessler JL (2002) Langmuir 18:6896–6906

    Article  CAS  Google Scholar 

  34. Bowman WD, Spiro TG (1981) Biochemistry 20:3313–3318

    Article  CAS  Google Scholar 

  35. Abe M, Kyogoku Y, Kitagawa T, Kawano K, Ohishi N, Takai-Suzuki A, Yagi K (1986) Spectrochim Acta 42A:1059–1068

    CAS  Google Scholar 

Download references

Acknowledgements

Financial support of the Czech Science Foundation, project No. P206/11/0951 and specific university research (MSMT No. 21/2011-A2_FCHI_2011_010) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Institute of Chemical Technology Prague, Technicka 5, 166 28, Prague 6, Czech Republic

    A. Kokaislová & P. Matějka

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  1. A. Kokaislová
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  2. P. Matějka
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Correspondence to A. Kokaislová.

Additional information

Published in the special issue Euroanalysis XVI (The European Conference on Analytical Chemistry) with Guest Editor Slavica Ražić.

Awarded an ABC Poster Prize on the occasion of the Euroanalysis XVI Conference in Belgrade, Serbia.

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Kokaislová, A., Matějka, P. Surface-enhanced vibrational spectroscopy of B vitamins: what is the effect of SERS-active metals used?. Anal Bioanal Chem 403, 985–993 (2012). https://doi.org/10.1007/s00216-011-5704-x

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  • DOI: https://doi.org/10.1007/s00216-011-5704-x

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