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Biodiversity and Biogeography of Fusarium Species from Northeastern North American Asparagus Fields Based on Microbiological and Molecular Approaches

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Abstract

Sixteen Fusarium species were recovered from 52 asparagus commercial fields, representing all major ecological (edaphic and climatic) area of asparagus production in the province of Québec, eastern Canada. This study extends our understanding of the geographic range of these species. It also provides climatological and edaphic properties linked to community changes and adaptations. Fusarium oxysporum and F. proliferatum were omnipresent and abundant in all five ecological area under study, whereas F. redolens was less frequently found. Species of Fusarium that produce carmine red pigmentation on potato dextrose agar, i.e., F. acuminatum, F. avenaceum, etc., were common at the northern limit of asparagus production. Abundance of red Fusarium species corresponded with a low isolation frequency of F. proliferatum. Nevertheless, F. proliferatum had a high recovery rate throughout Québec asparagus growing areas, under climatic conditions as cold as those of northern Europe where this species is uncommon in asparagus fields. In the light of these results, redefinition of the geographical distribution of F. proliferatum in asparagus fields is proposed. Intraspecific molecular differences in F. proliferatum and F. oxysporum were detected in the EF-1 alpha sequences and compared with well-characterized strains of North America.

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References

  1. GM Armstrong JK Armstrong (1966) ArticleTitleRelationships of Fusarium oxysporum formae speciales apii, aspargi, cassiae, melongenae and vasinectum race 3 as revealed by pathogenicity for common hosts Phytopathology 56 1256–1260

    Google Scholar 

  2. RP Baayen PHJF Boogert Particlevan den PJM Bonants JTK Poll WJ Blok C Waalwijk (2000) ArticleTitleFusarium redolens f. sp. asparagi, causal agent of asparagus root rot, crown rot and spear rot Eur J Plant Pathol 106 907–912 Occurrence Handle10.1023/A:1008766707266

    Article  Google Scholar 

  3. D Backhouse LW Burgess (2002) ArticleTitleClimatic analysis of the distribution of Fusarium graminearum, F. pseudograminearum and F. culmorum on cereals in Australia Australas Plant Pathol 3 321–327

    Google Scholar 

  4. D Backhouse LW Burgess (1995) ArticleTitleMycogeography of Fusarium: climatic analysis of the distribution within Australia of Fusarium species in section Gibbosum Mycol Res 99 1218–1224

    Google Scholar 

  5. GL Bateman G Murray (2001) ArticleTitleSeasonal variations in populations of Fusarium species in wheat-field soil Appl Soil Ecol 18 117–128 Occurrence Handle10.1016/S0929-1393(01)00158-5

    Article  Google Scholar 

  6. WJ Blok GJ Bollen (1997) ArticleTitleHost specificity and vegetative compatibility of Dutch isolates of Fusarium oxysporum f. sp. asparagi Can J Bot 75 383–393

    Google Scholar 

  7. WJ Blok GJ Bollen (1995) ArticleTitleFungi on the roots and stem bases of asparagus in the Netherlands: species and pathogenicity Eur J Plant Pathol 101 15–24 Occurrence Handle10.1007/BF01876090

    Article  Google Scholar 

  8. NC Brady RR Weil (2001) The Nature and Properties of Soils Prentice-Hall Upper Saddle River, NJ, USA

    Google Scholar 

  9. LW Burgess (1981) General ecology of Fusaria PE Nelson TA Toussoun RJ Cook (Eds) Fusarium, Diseases, Biology, and Taxonomy Pennsylvania State University Press University Park, PA 225–235

    Google Scholar 

  10. LW Burgess CM Liddell BA Summerell (1988) Laboratory Manual for Fusarium Research EditionNumber2 University of Sydney Sydney

    Google Scholar 

  11. LW Burgess BA Summerell (1992) ArticleTitleMycogeography of Fusarium: survey of Fusarium species in subtropical and semi-arid grassland soils from Queensland, Australia Mycol Res 98 780–784

    Google Scholar 

  12. RM Clear SK Patrick (1999) ArticleTitleFusarium head blight pathogens isolated from fusarium-damaged kernels of wheat in western Canada, 1993 to 1998 Can J Plant Pathol 22 51–60

    Google Scholar 

  13. SI Cohen FD Heald (1941) ArticleTitleA wilt and root rot of asparagus caused by Fusarium oxysporum (Schlecht) Plant Dis Rep 25 503–509 Occurrence Handle1:CAS:528:DyaH38XhvFeksw%3D%3D

    CAS  Google Scholar 

  14. KS Elias RW Schneider MM Lear (1991) ArticleTitleAnalysis of vegetative compatibility groups in nonpathogenic population of Fusarium oxysporum isolated from symptomless tomato roots Can J Bot 69 2089–2094

    Google Scholar 

  15. WH Elmer (2003) ArticleTitleLocal and systemic effects of NaCl on root composition, rhizobacteria, and Fusarium crown and root rot of asparagus Phytopathology 93 186–192

    Google Scholar 

  16. WH Elmer (2001) Fusarium diseases of asparagus BA Summerell JF Leslie D Backhouse WL Bryden LW Burgess (Eds) Fusarium: Paul E. Nelson Memorial Symposium APS Press St. Paul, MN 248–262

    Google Scholar 

  17. WH Elmer (2001) ArticleTitleSeeds as vehicles for pathogen importation Biol Invasions 3 263–271 Occurrence Handle10.1023/A:1015217308477

    Article  Google Scholar 

  18. WH Elmer (1995a) ArticleTitleA single mating population of Gibberella fujikuroi (Fusarium proliferatum) predominates in asparagus fields in Connecticut, Massachusetts, and Michigan Mycologia 87 68–71

    Google Scholar 

  19. WH Elmer (1995) ArticleTitleAssociation between Mn-reducing root bacteria and NaCl applications in suppression of Fusarium crown and root rot of asparagus Phytopathology 85 1461–1467

    Google Scholar 

  20. WH Elmer (1991) ArticleTitleVegetative compatibility groups of Fusarium proliferatum from asparagus and comparisons of virulence, growth rates, and asparagus residue colonization among groups Phytopathology 81 852–857

    Google Scholar 

  21. WH Elmer DA Johnson GI Mink (1996) ArticleTitleEpidemiology and management of diseases causal to asparagus decline Plant Dis 80 117–125

    Google Scholar 

  22. WH Elmer BA Summerell LW Burgess D Backhouse AA Abubacker (1997) ArticleTitleFusarium species associated with asparagus crowns and soil in Australia and New Zealand Australas Plant Pathol 26 255–261

    Google Scholar 

  23. WH Elmer CT Stephens (1989) ArticleTitleClassification of Fusarium oxysporum f. sp. asparagi into vegetatively compatible groups Phytopathology 79 88–93

    Google Scholar 

  24. WH Elmer CT Stephens (1989) ArticleTitleClassification of Fusarium oxysporum f. sp. asparagi into vegetatively compatible groups Phytopathology 79 88–93

    Google Scholar 

  25. D Fravel C Olivain C Alabouvette (2003) ArticleTitleFusarium oxysporum and its biocontrol New Phytol 157 493–502 Occurrence Handle10.1046/j.1469-8137.2003.00700.x

    Article  Google Scholar 

  26. W Gerlach H Nirenberg (1982) The Genus Fusarium—a Pictorial Atlas. Mittelungen aus der Biologishen Bundesansalt für Land-und Forstwirtschaft, Heft 209 Verlag Paul Parey Berlin

    Google Scholar 

  27. H Germain G Laflamme L Bernier B Boulet RC Hamelin (2002) ArticleTitleDNA polymorphism and molecular diagnosis in Inonotus spp Can J Plant Pathol 24 194–199 Occurrence Handle1:CAS:528:DC%2BD38Xlt1OktLs%3D

    CAS  Google Scholar 

  28. WL Gordon (1952) ArticleTitleThe occurrence of Fusarium species in Canada. II. Prevalence and taxonomy of Fusarium species in cereal seed Can J Bot 30 209–251

    Google Scholar 

  29. KM Graham (1955) ArticleTitleSeedling blight, a fusarial disease of asparagus Can J Bot 33 374–400

    Google Scholar 

  30. RG Grogan KA Kimble (1959) ArticleTitleThe association of Fusarium wilt and root rot with the asparagus decline and replant problem in California Phytopathology 49 122–125

    Google Scholar 

  31. AD Kent EW Triplett (2002) ArticleTitleMicrobial communities and their interactions in soil and rhizosphere ecosystems Annu Rev Microbiol 56 211–236 Occurrence Handle10.1146/annurev.micro.56.012302.161120 Occurrence Handle1:CAS:528:DC%2BD38Xos1Gis7s%3D Occurrence Handle12142496

    Article  CAS  PubMed  Google Scholar 

  32. JF Leslie CAS Pearson PE Nelson TA Tousson (1990) ArticleTitleFusarium spp. from corn, sorghum, and soybean fields in the central and eastern United States Phytopathology 80 343–350

    Google Scholar 

  33. J Louvet C Alabouvette F Rouxel (1981) Microbiological suppressiveness of some soils to Fusarium wilts PE Nelson TA Toussoun RJ Cook (Eds) Fusarium—Diseases, Biology and Taxonomy Pennsylvania State University Press University Park, PA 261–275

    Google Scholar 

  34. WFO Marasas LW Burgess RY Anelich SC Lamprecht DJ Schalkwyk Particlevan (1988) ArticleTitleSurvey of Fusarium species associated with plant debris in South African soils S Afr J Bot 54 63–71

    Google Scholar 

  35. DG Maynard YP Kalra (1993) Nitrate and exchangeable ammonium nitrogen MR Carter (Eds) Soil Sampling and Methods of Analysis Canadian Society of Soil Science, Lewis Publishers Boca Raton, FL 25–38

    Google Scholar 

  36. PE Nelson TA Toussoun WFO Marasas (1983) Fusarium Species: An Illustrated Manual for Identification Pennsylvania State University Press University Park, PA

    Google Scholar 

  37. K O'Donnell (2000) ArticleTitleMolecular phylogeny of the Nectria haematococca–Fusarium solani species complex Mycologia 92 919–938

    Google Scholar 

  38. K O'Donnell HC Kistler E Cigelnik R Ploetz (1998) ArticleTitleMultiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene geanologies Proc Natl Acad Sci 95 2044–2049 Occurrence Handle10.1073/pnas.95.5.2044 Occurrence Handle9482835

    Article  PubMed  Google Scholar 

  39. C Sadowski M Knaflewski (1990) ArticleTitleSusceptibility of selected asparagus cultivars to Fusarium spp. under field conditions Acta Hortic 27 343–352

    Google Scholar 

  40. AE Sangalang LW Burgess D Backhouse J Duff M Wurst (1995) ArticleTitleMycogeography of Fusarium species in soils from tropical, arid and mediterranean regions of Australia Mycol Res 99 523–528

    Google Scholar 

  41. S Sanogo XB Yang (2000) ArticleTitleRelation of sand content, pH, potassium and phosphorus nutrition to the development of sudden death syndrome in soybean Can J Plant Pathol 23 174–180

    Google Scholar 

  42. Saremi, H, Backhouse, D, Burgess, LW (1997) Mycogeographic survey of Fusarium species in southeastern New South Wales. Fifth European Fusarium Seminar. Szeged, Hungary, pp 611–612

  43. Saremi, H, Backhouse, D, Burgess, LW (1997) Soil environment effects on competition between Fusarium species. Fifth European Fusarium Seminar. Szeged, Hungary, pp 613–614

  44. W Schreuder SC Lamprecht WFO Marasas FJ Calitz (1995) ArticleTitlePathogenicity of three Fusarium species associated with asparagus decline in South Africa Plant Dis 79 177–181

    Google Scholar 

  45. T Sen Tran RR Simard (1993) Mehlich III-extractable elements MR Carter (Eds) Soil Sampling and Methods of Analysis Canadian Society of Soil Science, Lewis Publishers Boca Raton, FL 39–42

    Google Scholar 

  46. BH Sheldrick C Wang (1993) Particle size distribution MR Carter (Eds) Soil Sampling and Methods of Analysis Canadian Society of Soil Science, Lewis Publishers Boca Raton, USA 499–512

    Google Scholar 

  47. BA Summerell B Salleh JF Leslie (2003) ArticleTitleA utilitarian approach to Fusarium identification Plant Dis 87 117–128

    Google Scholar 

  48. DL Swofford (2000) PAUP (and Other Methods). Phylogenetic Analysis Using Parsimony Sinauer Associates Sunderland, MA

    Google Scholar 

  49. MA Tabatabai (1982) Soil enzymes AL Page (Eds) Methods of Soil Analysis American Society of Agronomy Madison, WI 903–947

    Google Scholar 

  50. JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 24 4876–4882

    Google Scholar 

  51. U Thrane (1989) Fusarium species and their specific profiles of secondary metabolites J Chelkowski (Eds) Fusarium: Mycotoxins, Taxonomy and Pathogenicity Elsevier Amsterdam 199–225

    Google Scholar 

  52. H Tissen JO Moir (1993) Total and organic carbon MR Carter (Eds) Soil Sampling and Methods of Analysis Canadian Society of Soil Science, Lewis Publishers Boca Raton, FL 187–200

    Google Scholar 

  53. JMM Bakel ParticleVan JJK Kertsen (1970) ArticleTitleFoot rot in asparagus caused by Fusarium oxysporum f. sp. asparagi Neth J Phytopathol 76 320–325

    Google Scholar 

  54. RP Voroney JP Winter RP Beyaert (1993) Soil microbial biomass C and N MR Carter (Eds) Soil Sampling and Methods of Analysis Canadian Society of Soil Science, Lewis Publishers Boca Raton, FL 277–287

    Google Scholar 

  55. V Vujanovic C Hamel S Jabaji-Hare M St-Arnaud (2002) ArticleTitleDevelopment of a selective myclobutanil agar (MBA) medium for the isolation of Fusarium species from asparagus fields Can J Microbiol 48 841–847 Occurrence Handle10.1139/w02-082 Occurrence Handle1:CAS:528:DC%2BD38XovVaqtrs%3D Occurrence Handle12455616

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This research was financially supported by a CORPAQ grant. The authors are thankful to Dr Wade Elmer, phytopathologist from the Connecticut Agricultural Experiment Station, New Haven, CT, USA, for the loan of US Fusarium specimens as well as for manuscript review and useful comments. We thank Dr. Robert Baayen, from the Plant Protection Service, Mycology Section, Wageningen, The Netherlands, and Dr Lily Tamburic-Ilincic, from the University of Guelph, Guelph, Ontario, Canada, for providing fungal strains. We also thank Jean-Guy Tessier, agronomist from the MAPAQ, for useful professional advice, and Houda Jallal, Université de Montréal, for technical assistance.

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  1. Vladimir Vujanovic

    Present address: Applied Microbiology and Food Science, College of Agriculture, University of Saskatchewan, Canada

Authors and Affiliations

  1. Institut de Recherche en Biologie Végétale, Université de Montréal & Jardin botanique de Montréal, Montreal, Canada

    Vladimir Vujanovic, Etienne Yergeau & Marc St-Arnaud

  2. Environmental Health/Water and Nutrients, Agriculture and Agri-Food Canada, Swift Current, Saskatchewan, Canada

    Chantal Hamel

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  1. Vladimir Vujanovic
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Correspondence to Vladimir Vujanovic or Marc St-Arnaud.

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Vujanovic, V., Hamel, C., Yergeau, E. et al. Biodiversity and Biogeography of Fusarium Species from Northeastern North American Asparagus Fields Based on Microbiological and Molecular Approaches. Microb Ecol 51, 242–255 (2006). https://doi.org/10.1007/s00248-005-0046-x

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