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Overexpression of wheat α-mannosidase gene TaMP impairs salt tolerance in transgenic Brachypodium distachyon

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Abstract

Key message

The TaMP gene from wheat encodes an α-mannosidase induced by salt stress that functions as negative regulator of salt tolerance in plants.

Abstract

Salt stress significantly affects growth and yield of crop plants. The α-mannosidases function in protein folding, trafficking, and endoplasmic reticulum-associated degradation in eukaryotic cells, and they are involved in abiotic stress tolerance in plants. Previously, we identified the α-mannosidase gene TaMP in wheat (Triticum aestivum). In this study, we investigated the function of TaMP in salt stress tolerance. TaMP expression was induced in wheat leaves by salt, drought, abscisic acid, and H2O2 treatments. Overexpressing TaMP in Brachypodium distachyon was associated with a salt-sensitive phenotype. Under salt stress, the overexpressing plants had reduced height, delayed growth status, low photosynthetic rate, decreased survival rate, and diminished yield. Moreover, the overexpression of TaMP aggravated the tendency for ions to become toxic under salt stress by significantly affecting the Na+ and K+ contents in cells. In addition, TaMP could negatively regulate salt tolerance by affecting the antioxidant enzyme system capacity and increasing the reactive oxygen species accumulation. Our study was helpful to understand the underlying physiological and molecular mechanisms of salt stress tolerance in plants.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (no. 31370304) and by Funds of Shandong “Double Tops” program.

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  1. Wenlong Wang and Yunzhen Wu have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Wenlong Wang, Yunzhen Wu, Ruirui Shi, Mengwei Sun, Qinxue Li, Guangqiang Zhang, Jiajie Wu, Yong Wang & Wei Wang

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  1. Wenlong Wang
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The work presented here was carried out in collaboration among all authors. WLW conceived and designed the study. YZW, RRS, MWS, QXL, GQZ, JJW, and YW performed experiments and analyzed data. WLW wrote the manuscript. WW provided vital advice on the article. All authors approved the manuscript.

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Correspondence to Wei Wang.

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299_2020_2522_MOESM2_ESM.tif

Fig. S1 The expression level of TaMP and the α-mannosidases activity in the transgenic lines. (a) The expression level of TaMP in transgenic lines. (b) The activity of in transgenic Brachypodium distachyon and WT plants. The data were presented as the mean ±SD of three independent experiments (n=3). Asterisks above each column indicate statistical differences to the WT plants (*P<0.05; **P<0.01) (TIF 8156 kb)

299_2020_2522_MOESM3_ESM.tif

Fig. S2 The expression profile of TaMP and the phenotypes of transgenic lines under under ER stress. (a-b) The profile of TaMP with or without 10 mM DTT treatment. Ten-day-old wheat seedlings were treated with the respective stress-inducing agents for different time points, after which the leaves were collected and used for TaMP expression analysis. (c) The phenotypes of transgenic and WT lines without or with 10 mM DTT. The data were presented as the mean ±SD of three independent experiments (TIF 7473 kb)

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Wang, W., Wu, Y., Shi, R. et al. Overexpression of wheat α-mannosidase gene TaMP impairs salt tolerance in transgenic Brachypodium distachyon. Plant Cell Rep 39, 653–667 (2020). https://doi.org/10.1007/s00299-020-02522-2

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