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Endophytic Alcaligenes Isolated from Horticultural and Medicinal Crops Promotes Growth in Okra (Abelmoschus esculentus)

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Abstract

The potential of endophytic bacteria to act as biofertilizers and bioprotectants has been demonstrated, and considerable progress has been made in explaining their role in plant protection. In the present study, three endophytic bacterial strains (BHU 12, BHU 16 isolated from the leaves of Abelmoschus esculentus, and BHU M7 isolated from the leaves of Andrographis paniculata) were used which displayed high sequence similarity to Alcaligenes faecalis. The biofilm formation ability of these endophytic strains in the presence of okra root exudates confirms their chemotactic ability, an initial step for successful endophytic colonization. Further, reinoculation of spontaneous rifampicin-tagged mutants into okra seedlings revealed a CFU count above 105 cells g−1 of all three endophytic strains in root samples during the first 15 days of plant growth. The CFU count increased up to 1013 by 30 days of plant growth, followed by a gradual decline to approximately 1010 cells g−1 at 45 days of plant growth. Systemic endophytic colonization was further supported by 2, 3, 5-triphenyl tetrazolium chloride staining and fluorescence imaging of ds-RED expressing conjugants of the endophytic strains. The strains were further assessed for their plausible in vivo and in vitro plant growth-promoting and antagonistic abilities. Our results demonstrated that the endophytic strains BHU 12, BHU 16, and BHU M7 augmented plant biomass by greater than 40 %. Root and shoot lengths of okra plants when primed by BHU 12, BHU 16, and BHU M7 increased up to 34 and 14.5 %, respectively. The endophytic isolates also exhibited significant in vitro antagonistic potential against the collar rot pathogen Sclerotium rolfsii. In summary, our results demonstrate excellent potential of the three endophytic bacterial strains as biofertilizers and biocontrol agents, indicating the possibility for use in sustainable agriculture.

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Acknowledgments

Shatrupa Ray is thankful to Banaras Hindu University, Varanasi, for award of RET-UGC fellowship. HBS and BKS are grateful to Indian Council of Agricultural Research for providing financial assistance under ICAR-Seed Project scheme.

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Authors and Affiliations

  1. Department of Botany, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India

    Shatrupa Ray & Surendra Singh

  2. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India

    B. K. Sarma & H. B. Singh

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  1. Shatrupa Ray
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  2. Surendra Singh
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  3. B. K. Sarma
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  4. H. B. Singh
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Correspondence to H. B. Singh.

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Ray, S., Singh, S., Sarma, B.K. et al. Endophytic Alcaligenes Isolated from Horticultural and Medicinal Crops Promotes Growth in Okra (Abelmoschus esculentus). J Plant Growth Regul 35, 401–412 (2016). https://doi.org/10.1007/s00344-015-9548-z

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