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The plasma membrane H+-ATPase is related to the development of salicylic acid-induced thermotolerance in pea leaves

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Abstract

The plasma membrane H+-ATPase (PM H+-ATPase, EC.3.6.1.35) plays a key role in the plant response to environmental stress. In this study, a possible mechanistic link between the PM H+-ATPase and salicylic acid (SA)-induced thermotolerance was investigated in pea (Pisum sativum L. cv. NingXia) leaves. The burst of free SA in response to heat acclimation (38 ± 0.5°C) was observed, and peaks appeared subsequently both in activity and amount of PM H+-ATPase in pea leaves during heat acclimation. Similarly, exogenous SA also triggered the two peaks in the room temperature (25 ± 0.5°C). Paclobutrazol (PAC) was employed to infiltrate onto pea leaves prior to heat acclimation treatment. The results showed that the peaks of both free SA and activity of PM H+-ATPase still occurred after the PAC pretreatment. In acquired thermotolerance assessment (malondialdehyde content and degree of wilting), spraying SA and fusicoccin (FC, the activator of PM H+-ATPase) separately could protect pea leaves from heat injury. Results from RT-PCR and western blotting analysis indicated that the increase in activity of the PM H+-ATPase was due to its transcriptional and translational regulation. The subcellular localizations of PM H+-ATPase after the FC or SA pretreatment also showed that the PM H+-ATPase is important to maintain the integrity of plasma membrane against the heat stress. Taken together, these results suggest PM H+-ATPase is related to the development of SA-induced thermotolerance in pea leaves.

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Abbreviations

DTT:

Dithiothreitol

FC:

Fusicoccin

MDA:

Malondialdehyde

NBT/BCIP:

Nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate

PAC:

Paclobutrazol

PIP2-PLC:

Phosphatidylinositol-4, 5-bisphosphate-specific phospholipase C

PM:

Plasma membrane

PM H+-ATPase:

Plasma membrane H+-ATPase

PMSF:

Phenylmethylsulfonyl

PVPP:

Polyvinylpyrrolidone

RT-PCR:

Reverse transcription-polymerase chain reactions

SA:

Salicylic acid

SAG:

Salicylic acid 2-O-β-D-glucose

SAR:

Systemic acquired resistance

TCA:

Trichloroacetic acid

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Acknowledgments

We are grateful to Dr. R. Serrano and Dr. Jennifer Corrigan for their kindly providing the antibody against PM H+-ATPase and Hsps. This research was supported by the National Natural Science Foundation of China (Grant Numbers 30471192 and 30671468), Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20050019015) and The Significant Program of Beijing Municipal Science and Technology Commission (No. D07060500160701).

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  1. College of Food Science and Nutritional Engineering, China Agricultural University, No.17, Qinghua Dong Lu, Haidian District, 100083, Beijing, China

    Yanyan Liu, Qiuhong Pan, Haoru Yang, Jicheng Zhan & Weidong Huang

  2. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, 100101, Beijing, China

    Hongtao Liu

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  1. Yanyan Liu
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Liu, Y., Liu, H., Pan, Q. et al. The plasma membrane H+-ATPase is related to the development of salicylic acid-induced thermotolerance in pea leaves. Planta 229, 1087–1098 (2009). https://doi.org/10.1007/s00425-009-0897-3

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