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Cross-linking of horseradish peroxidase adsorbed on polycationic films: utilization for direct dye degradation

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Abstract

Horseradish peroxidase (HRP) was immobilized on the polyaniline (PANI) grafted polyacrylonitrile (PAN) films. The maximum HRP immobilization capacity of the PAN-g-PANI-3 film was 221 μg/cm2. The HRP-immobilized PAN-g-PANI-3 film retained 79 % of the activity of the same quantity free enzyme. The HRP-immobilized PAN-g-PANI-3 film was operated for the decolorization of two different benzidine-based dyes in the presence of hydrogen peroxide. The maximum decolorization grade was obtained at pH 6.0 for both dyes. The HRP-immobilized PAN-g-PANI-3 film was very effective for removal of Direct Blue-53 compared to Direct Black-38 from aqueous solutions. The immobilized HRP exhibited high resistance to proteolysis by trypsin compared to the free counterpart. Immobilized HRP preserved 83 % of its original activity even after 8 weeks of storage at 4 °C, while the free enzyme lost its initial activity after 3 weeks of storage period.

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Authors and Affiliations

  1. Biochemical Processing and Biomaterial Research Laboratory, Faculty of Sciences, Gazi University, Teknik Okullar, 06500, Ankara, Turkey

    Gulay Bayramoglu, Begum Altintas & M. Yakup Arica

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  1. Gulay Bayramoglu
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  2. Begum Altintas
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  3. M. Yakup Arica
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Correspondence to Gulay Bayramoglu.

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Bayramoglu, G., Altintas, B. & Yakup Arica, M. Cross-linking of horseradish peroxidase adsorbed on polycationic films: utilization for direct dye degradation. Bioprocess Biosyst Eng 35, 1355–1365 (2012). https://doi.org/10.1007/s00449-012-0724-2

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  • DOI: https://doi.org/10.1007/s00449-012-0724-2

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