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Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation

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Abstract

This study investigated the effects of transformation conditions such as initial pH, the initial concentration of glucose and yeast extract in the medium, and the separate addition of ferulic acid and vanillic acid, on the production of vanillin through an analysis of competing by-product formation by Amycolatopsis sp. ATCC 39116. The extent and nature of by-product formation and vanillin yield were affected by initial pH and different initial concentrations of glucose and yeast extract in the medium, with a high yield of vanillin and high cell density obtained at pH 8.0, 10 g/l glucose, and 8 g/l yeast extract. High concentrations of ferulic acid were found to negatively affect cell density. Additional supplementation of 100 mg/l vanillic acid, a metabolically linked by-product, was found to result in a high concentration of vanillin and guaiacol, an intermediate of vanillin. Via an analysis of the effect of these transformation conditions on competing by-product formation, high concentrations of ferulic acid were transformed with a molar yield to vanillin of 96.1 and 95.2 %, by Amycolatopsis sp. ATCC 39116 and Streptomyces V1, respectively, together with a minor accumulation of by-products. These are among the highest performance values reported in the literature to date for Streptomyces in batch cultures.

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Acknowledgments

The Project was supported by Social Development and Scientific and Technological Plan in Shaanxi Province of China (Program No. 693102), Science and technology projects in Xi’an of China (No.694180) and the Fundamental Research Funds for the Central Universities of China (No.GK201003002). Many thanks to Julian Dafoe and Eric Peterson for their support during this study.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Shaanxi Normal University, 199 Chang’an South Road, Xi’an, 710055, Shaanxi, People’s Republic of China

    Xiao-kui Ma

  2. Department of Chemical Engineering, Queen’s University, Kingston, ON, K7L 3N6, Canada

    Andrew J. Daugulis

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  1. Xiao-kui Ma
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  2. Andrew J. Daugulis
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Correspondence to Andrew J. Daugulis.

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Ma, Xk., Daugulis, A.J. Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation. Bioprocess Biosyst Eng 37, 891–899 (2014). https://doi.org/10.1007/s00449-013-1060-x

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  • DOI: https://doi.org/10.1007/s00449-013-1060-x

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