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Kinetic model-based feed-forward controlled fed-batch fermentation of Lactobacillus rhamnosus for the production of lactic acid from Arabic date juice

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Abstract

Arabic date is overproduced in Arabic countries such as Saudi Arabia and Iraq and is mostly composed of sugars (70–80 wt%). Here we developed a fed-batch fermentation process by using a kinetic model for the efficient production of lactic acid to a high concentration from Arabic date juice. First, a kinetic model of Lactobacillus rhamnosus grown on date juice in batch fermentation was constructed in EXCEL so that the estimation of parameters and simulation of the model can be easily performed. Then, several fed-batch fermentations were conducted by employing different feeding strategies including pulsed feeding, exponential feeding, and modified exponential feeding. Based on the results of fed-batch fermentations, the kinetic model for fed-batch fermentation was also developed. This new model was used to perform feed-forward controlled fed-batch fermentation, which resulted in the production of 171.79 g l−1 of lactic acid with the productivity and yield of 1.58 and 0.87 g l−1 h−1, respectively.

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Abbreviations

A :

Feeding rate of the ammonia solution (l h−1)

a :

Growth associated lactic acid production factor

b :

Non-growth associated lactic acid production factor (h−1)

\(C_{\text{A}}\) :

Concentration of ammonia in the ammonia solution (g l−1)

c :

Toxic-power constant of the specific cell growth rate

d :

Toxic-power constant of the non-growth associated lactic acid production factor

F :

Feeding rate of the feeding solution (l h−1)

\(K_{\text{s}}\) :

Saturation constant in Monod equation (g l−1)

\( {\text{MW}}_{\text{A}} \) :

Molecular weight of ammonia (g mol−1)

\({\text{MW}}_{\text{LA}}\) :

Molecular weight of lactic acid (g mol−1)

P :

Concentration of lactic acid in a bioreactor (g l−1)

\(P_{\text{m}}\) :

Maximum lactic acid concentration for the cell growth (g l−1)

\(P'_{\text{m}}\) :

Maximum lactic acid concentration for lactic acid production (g l−1)

S :

Concentration of substrate in a bioreactor (g l−1)

S i :

Concentration of substrate in the feeding solution (g l−1)

t :

Culture time (h)

V :

Volume of a bioreactor (l)

V 0 :

Initial volume (l)

X :

Concentration of cell in a bioreactor (g l−1)

X 0 :

Initial concentration of cell (g l−1)

Y X/S :

Theoretical cell yield (g g−1)

Y P/S :

Theoretical lactic acid yield (g g−1)

\(\mu\) :

Specific growth rate (h−1)

\(\mu_{ \hbox{max} }\) :

Maximum specific growth rate (h−1)

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Acknowledgments

This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea (NRF-2012-C1AAA001-2012M1A2A2026557). Further support by King Saud University is appreciated.

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Authors and Affiliations

  1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, 305-701, Republic of Korea

    Minsung Choi & Sang Yup Lee

  2. Chemical Engineering Department, King Saud University, PO Box 800, Riyadh, 11421, Saudi Arabia

    Saeed M. Al-Zahrani

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  1. Minsung Choi
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  2. Saeed M. Al-Zahrani
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  3. Sang Yup Lee
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Correspondence to Sang Yup Lee.

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Choi, M., Al-Zahrani, S.M. & Lee, S.Y. Kinetic model-based feed-forward controlled fed-batch fermentation of Lactobacillus rhamnosus for the production of lactic acid from Arabic date juice. Bioprocess Biosyst Eng 37, 1007–1015 (2014). https://doi.org/10.1007/s00449-013-1071-7

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