Abstract
Key message
Short-term heat stress during male meiosis causes defects in crossover formation, meiotic progression and cell wall formation in the monocot barley, ultimately leading to pollen abortion.
Abstract
High temperature conditions cause a reduction of fertility due to alterations in meiotic processes and gametogenesis. The male gametophyte development has been shown to be particularly sensitive to heat stress, and even short-term and modest temperature shifts cause alterations in crossover formation. In line with previous reports, we observed that male meiosis in the monocot barley exposed for 24–45 h to heat stress (32–42 °C) partially or completely eliminates obligate crossover formation and causes unbalanced chromosome segregation and meiotic abortion. Depending on the severity of heat stress, the structure and organization of the chromosomes were altered. In addition to alterations in chromosome structure and dynamics, heat treatment abolished or reduced the formation of a callose wall surrounding the meiocytes and interrupted the cell cycle progression leading to cytokinesis defects and microspore cell death.
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All datasets and materials used in the current study are available from the corresponding author upon request.
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Acknowledgements
We would like to thank Jochen Kumlehn (IPK) for the gift of the barley seeds.
Funding
This work was supported by the Research Foundation—Flanders (FWO), through a Ph.D. fellowship to Cédric Schindfessel (Grant No. 11G7421N).
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Schindfessel, C., Drozdowska, Z., De Mooij, L. et al. Loss of obligate crossovers, defective cytokinesis and male sterility in barley caused by short-term heat stress. Plant Reprod 34, 243–253 (2021). https://doi.org/10.1007/s00497-021-00415-2
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DOI: https://doi.org/10.1007/s00497-021-00415-2