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Non-enzymatic sensing of glucose using a glassy carbon electrode modified with gold nanoparticles coated with polyethyleneimine and 3-aminophenylboronic acid

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Abstract

A non-enzymatic electrochemical method was developed for sensing glucose by using a glassy carbon electrode modified with 3-aminophenylboronic acid (APBA) immobilized on polyethyleneimine (PEI)-coated gold nanoparticles. The modified electrode was characterized by TEM, zeta potential measurements and UV-Vis spectroscopy. Its analytical performance was evaluated in pH 9 solution by potentiometry. The respective calibration plot, established at open circuit potential (vs. Ag/AgCl) covers the 0.5–50 mM glucose concentration range, which makes it suitable for blood glucose assays. The detection limit is 0.025 mM, and no interference is caused by ascorbic acid, dopamine, and uric acid. Effects of other carbohydrates such as fructose, galactose and saccharose were also investigated. The electrode was used to determine glucose in human serum samples and the results agreed well with those obtained with commercial amperometric enzymatic sensors.

Schematic of the preparation of polyethyleneimine (PEI) coated gold nanoparticles modified with 3-aminophenylboronic acid for use in non-enzymatic potentiometric determinaton of glucose based on molecular recognition

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Acknowledgments

The authors gratefully acknowledge the financial support from Kırıkkale University Research Fund through Grant no.: 2015/48.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Processing Technologies, Kırıkkale University, Kırıkkale Vocational High School, 71450, Kırıkkale, Turkey

    Hakan Çiftçi & Filiz Çelik

  2. Department of Chemical Engineering, Faculty of Engineering, Hitit University, 19030, Çorum, Turkey

    Erol Alver

  3. Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, 71450, Kırıkkale, Turkey

    Ayşegül Ü. Metin

  4. Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey

    Uğur Tamer

Authors
  1. Hakan Çiftçi
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  2. Erol Alver
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  4. Ayşegül Ü. Metin
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  5. Uğur Tamer
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Correspondence to Hakan Çiftçi.

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Çiftçi, H., Alver, E., Çelik, F. et al. Non-enzymatic sensing of glucose using a glassy carbon electrode modified with gold nanoparticles coated with polyethyleneimine and 3-aminophenylboronic acid. Microchim Acta 183, 1479–1486 (2016). https://doi.org/10.1007/s00604-016-1782-y

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  • DOI: https://doi.org/10.1007/s00604-016-1782-y

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