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A bimetallic (Co/2Fe) metal-organic framework with oxidase and peroxidase mimicking activity for colorimetric detection of hydrogen peroxide

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Abstract

A bimetallic metal-organic framework of the type MOF(Co/2Fe), synthesized by a hydrothermal method, is demonstrated to exhibit peroxidase-like activity and oxidase-like activity. The material catalyzes the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) to produce a blue product both in the absence and presence of H2O2. Its catalytic activity strongly depends on temperature and the solution pH value. With its dual enzyme mimetic activity, MOF(Co/2Fe) has the advantages of low cost, good stability, ease of preparation, and attractive Michaelis-Menten behavior. The mechanisms and kinetics of the pseudo-enzymatic activity were studied in some detail. A colorimetric method was developed for determination of H2O2 that is based on the peroxidase-like activity of MOF(Co/2Fe). Under the optimal conditions, the absorbance of oxidized TMB at 652 nm increases linearly in the 10 to 100 μM H2O2 concentration range, with a 5 μM limit of detection.

A bimetallic metal-organic framework of the type MOF(Co/2Fe) exhibits peroxidase-like activity and oxidase-like activity. The material catalyzes the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) to produce a blue product in the presence and absence of H2O2.

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Acknowledgements

The financial support from the National Natural Science Foundation of China (21365005), Guangxi Natural Science Foundation of China (2014GXNSFGA118002) and the BAGUI Scholar Program is gratefully acknowledged.

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Author notes

  1. Haiguan Yang and Ruitao Yang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, People’s Republic of China

    Haiguan Yang, Ruitao Yang, Peng Zhang, Yuemei Qin, Tao Chen & Fanggui Ye

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  1. Haiguan Yang
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  2. Ruitao Yang
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  3. Peng Zhang
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  4. Yuemei Qin
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  6. Fanggui Ye
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Correspondence to Fanggui Ye.

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Yang, H., Yang, R., Zhang, P. et al. A bimetallic (Co/2Fe) metal-organic framework with oxidase and peroxidase mimicking activity for colorimetric detection of hydrogen peroxide. Microchim Acta 184, 4629–4635 (2017). https://doi.org/10.1007/s00604-017-2509-4

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  • DOI: https://doi.org/10.1007/s00604-017-2509-4

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