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Digital imaging information technology for biospeckle activity assessment relative to bacteria and parasites

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Abstract

This paper reports on the biospeckle processing of biological activity using a visualization scheme based upon the digital imaging information technology. Activity relative to bacterial growth in agar plates and to parasites affected by a drug is monitored via the speckle patterns generated by a coherent source incident on the microorganisms. We present experimental results to demonstrate the potential application of this methodology for following the activity in time. The digital imaging information technology is an alternative visualization enabling the study of speckle dynamics, which is correlated to the activity of bacteria and parasites. In this method, the changes in Red-Green-Blue (RGB) color component density are considered as markers of the growth of bacteria and parasites motility in presence of a drug. The RGB data was used to generate a two-dimensional surface plot allowing an analysis of color distribution on the speckle images. The proposed visualization is compared to the outcomes of the generalized differences and the temporal difference. A quantification of the activity is performed using a parameterization of the temporal difference method. The adopted digital image processing technique has been found suitable to monitor motility and morphological changes in the bacterial population over time and to detect and distinguish a short term drug action on parasites.

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Acknowledgements

The authors thank the Arteche brothers from CEADEN who collaborated in the automation of the setup presented. H. Cabrera gratefully acknowledges ICTP TRIL as well as the Associateship Programmes.

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Author notes

  1. Evelio E. Ramírez-Miquet

    Present address: Laboratoire Matériaux et Phénomènes Quantiques, Université Paris Diderot, CNRS UMR 7162, Sorbonne Paris-Cité, 10 rue Alice Domon et Léonie Duquet, Paris, 75013, France

Authors and Affiliations

  1. Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Calle 30 No. 502, Miramar C.P., 11300, La Habana, Cuba

    Evelio E. Ramírez-Miquet, Isabel Otero, Dania Rodríguez & Juan G. Darias

  2. Centro Multidisciplinario de Ciencias, Instituto Venezolano de Investigaciones Científicas, Mérida 5101, Venezuela and SPIE-ICTP Research in Optics Program Laboratory, International Centre for Theoretical Physics (ICTP), Strada Costiera 11, Trieste, 34151, Italy

    Humberto Cabrera

  3. Facultad de Farmacia y Bioanálisis, Universidad de los Andes, Mérida, 5101, Venezuela

    Hilda C. Grassi & Efrén de J. Andrades

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  1. Evelio E. Ramírez-Miquet
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  2. Humberto Cabrera
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  3. Hilda C. Grassi
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Correspondence to Evelio E. Ramírez-Miquet.

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Ramírez-Miquet, E.E., Cabrera, H., Grassi, H.C. et al. Digital imaging information technology for biospeckle activity assessment relative to bacteria and parasites. Lasers Med Sci 32, 1375–1386 (2017). https://doi.org/10.1007/s10103-017-2256-0

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  • DOI: https://doi.org/10.1007/s10103-017-2256-0

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