Skip to main content
SpringerLink
Log in

Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being adsorbed at 40°C but only 10% of initial hIgG at 25°C. At pH 5 the maximum adsorbed amount (20 mg hIgG/g support) was similar for both temperatures. The adsorption of hIgG on to charged poly(methyl methacrylate)/poly(N-isopropylacrylamide) core-shell latexes was negligible (5–10 mg hIgG/g support) at the same temperature and pH conditions. The lower adsorption of hIgG onto the core-shell particles is explained by steric interactions due to the small size of the shell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Baptista RP, Santos AM, Fedorov A, Martinho JM, Pichot C, Elaissari A, Cabral JM, Taipa MA (2003) Activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles. J Biotechnol 102(3):241–249

    Article  PubMed  CAS  Google Scholar 

  • Duracher D, Sauzedde F, Elaissari A, Perrin A, Pichot C (1998a) Cationic amino-containing N-isopropylacrylamide-styrene copolymer latex particles: 1–Particle size and morphology vs. polymerization process. Colloid Polym Sci 276(3):219–231

    Article  CAS  Google Scholar 

  • Fong RB, Ding Z, Hoffman AS, Stayton PS (2002) Affinity separation using an Fv antibody fragment-“smart” polymer conjugate. Biotechnol Bioeng 79(3):271–276

    Article  PubMed  CAS  Google Scholar 

  • Galaev IY, Mattiasson B (1999) ‘Smart’ polymers and what they could do in biotechnology and medicine. Trends Biotechnol 17(8):335–340

    Article  PubMed  CAS  Google Scholar 

  • Gil ES, Hudson SA (2004) Stimuli-reponsive polymers and their bioconjugates. Prog Polym Sci 29(12):1173–1222

    Article  CAS  Google Scholar 

  • Hilbrig F, Freitag R (2003) Protein purification by affinity precipitation. J Chromatogr B Analyt Technol Biomed Life Sci 790(1–2):79–90

    PubMed  CAS  Google Scholar 

  • Hoffman AS (2000) Bioconjugates of intelligent polymers and recognition proteins for use in diagnostics and affinity separations. Clin Chem 46(9):1478–1486

    PubMed  CAS  Google Scholar 

  • Hoffman AS (2002) Hydrogels for biomedical applications. Adv Drug Deliv Rev 54(1):3–12

    Article  PubMed  CAS  Google Scholar 

  • Kawaguchi H, Fujimoto K, Mizuhara Y (1992) Hydrogel microspheres.3. temperature-dependent adsorption of proteins on poly-N-isopropylacrylamide hydrogel microspheres. Colloid Polym Sci 270(1):53–57

    Article  CAS  Google Scholar 

  • Kim JY, Mulchandani A, Chen W (2005) Temperature-triggered purification of antibodies. Biotechnol Bioeng 90(3):373–379

    Article  PubMed  CAS  Google Scholar 

  • Kumar A, Wahlund PO, Kepka C, Galaev IY, Mattiasson B (2003) Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers. Biotechnol Bioeng 84(4):494–503

    Article  PubMed  CAS  Google Scholar 

  • Pelton R (2000) Temperature-sensitive aqueous microgels. Adv Colloid Interface Sci 85(1):1–33

    Article  PubMed  CAS  Google Scholar 

  • Roque AC, Lowe CR, Taipa MA (2004) Antibodies and genetically engineered related molecules: production and purification. Biotechnol Prog 20(3):639–654

    Article  PubMed  CAS  Google Scholar 

  • Roque AC, Taipa MA, Lowe CR (2005a) An artificial protein L for the purification of immunoglobulins and fab fragments by affinity chromatography. J Chromatogr A 1064(2):157–167

    Article  CAS  Google Scholar 

  • Roque AC, Taipa MA, Lowe CR (2005b) Synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein L from Peptostreptococcus magnus. J Mol Recognit 18(3):213–224

    Article  CAS  Google Scholar 

  • Santos AM, Elaissari A, Martinho JMG, Pichot C (2005) Synthesis of cationic poly(methyl methacrylate)-poly(N-isopropyl acrylamide) core-shell latexes via two-stage emulsion copolymerization. Polymer 46(4):1181–1188

    Article  CAS  Google Scholar 

  • Taipa MA, Kaul RH, Mattiasson B, Cabral JM (2000) Recovery of a monoclonal antibody from hybridoma culture supernatant by affinity precipitation with Eudragit S-100. Bioseparation 9(5):291–298

    Article  PubMed  CAS  Google Scholar 

  • Taniguchi T, Duracher D, Delair T, Elaissari A, Pichot C (2003) Adsorption/desorption behavior and covalent grafting of an antibody onto cationic amino-functionalized poly(styrene-N-isopropylacrylamide) core-shell latex particles. Colloids Surf B 29(1):53–65

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financed by the Fundação para a Ciência e a Tecnologia (FCT), Portugal, under the projects POCTI/2000/QUI/33866 and POCTI/BIO/45296/2002. A research fellowship to Claudia S.O. Silva is also acknowledged.

Author information

Authors and Affiliations

  1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1049-001, Lisbon, Portugal

    Claudia S. O. Silva, Ricardo P. Baptista, Joaquim M. S. Cabral & M. Ângela Taipa

  2. Centro de Química-Física Molecular, Complexo I, Instituto Superior Técnico, Av Rovisco Pais, 1049-001, Lisbon, Portugal

    Andrea M. Santos & José M. G. Martinho

Authors
  1. Claudia S. O. Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ricardo P. Baptista
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrea M. Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. José M. G. Martinho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Joaquim M. S. Cabral
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Ângela Taipa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Ângela Taipa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Silva, C.S.O., Baptista, R.P., Santos, A.M. et al. Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles. Biotechnol Lett 28, 2019–2025 (2006). https://doi.org/10.1007/s10529-006-9188-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-006-9188-2

Keywords

Search

Navigation