Skip to main content
SpringerLink
Log in

In vitro formation of lacuna structure by human dermal fibroblasts co-cultured with porcine chondrocytes on a 3D biodegradable scaffold

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Dermal fibroblasts (DF) possess chondrogenic differentiation potential but whether DF, or a subpopulation of DF, can form a typical cartilage structure in culture is unknown. In this study, human DF were co-cultured with porcine articular chondrocytes on a biodegradable scaffold of polylactic acid/polyglycolic acid. Histological analysis demonstrated that some DFs can be induced to form cartilage lacuna structure showing the existence of a chondrogenic subpopulation of human DF. Moreover, the 3D-co-culture system can serve as an optimal model for directing stem cell differentiation in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bartsch G, Yoo JJ, De Coppi P, Siddiqui MM, Schuch G, Pohl HG, Fuhr J, Perin L, Soker S, Atala A (2005) Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors. Stem Cells Dev 14:337–348

    Article  PubMed  CAS  Google Scholar 

  • Chen FG, Zhang WJ, Bi D, Liu W, Wei X, Chen FF, Zhu L, Cui L, Cao Y (2007) Clonal analysis of nestin− vimentin+ multipotent fibroblasts isolated from human dermis. J Cell Sci, in press

  • DeLise AM, Stringa E, Woodward WA, Mello MA, Tuan RS (2000) Embryonic limb mesenchyme micromass culture as an in vitro model for chondrogenesis and cartilage maturation. Methods Mol Biol 137:359–375

    PubMed  CAS  Google Scholar 

  • French MM, Rose S, Canseco J, Athanasiou KA (2004) Chondrogenic differentiation of adult dermal fibroblasts. Ann Biomed Eng 32:50–56

    Article  PubMed  CAS  Google Scholar 

  • Harford JB (2002) Establishment of fibroblast cultures. In: Bonifacino JS et al (eds) Current protocols in cell biology. John Wiley & Sons, Inc, New York

    Google Scholar 

  • Heng BC, Cao T, Lee EH (2004) Directing stem cell differentiation into the chondrogenic lineage in vitro. Stem Cells 22:1152–1167

    Article  PubMed  Google Scholar 

  • Jones EA, English A, Henshaw K, Kinsey SE, Markham AF, Emery P, McGonagle D (2004) Enumeration and phenotypic characterization of synovial fluid multipotential mesenchymal progenitor cells in inflammatory and degenerative arthritis. Arthritis Rheum 50:817–827

    Article  PubMed  Google Scholar 

  • Jones EA, Kinsey SE, English A, Jones RA, Straszynski L, Meredith DM, Markham AF, Jack A, Emery P, McGonagle D (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum 46:3349–3360

    Article  PubMed  Google Scholar 

  • Kim BS, Yoo SP, Park HW (2004) Tissue engineering of cartilage with chondrocytes cultured in a chemically-defined, serum-free medium. Biotechnol Lett 26:709–712

    Article  PubMed  CAS  Google Scholar 

  • Mizuno S, Glowacki J (2005) Low oxygen tension enhances chondroinduction by demineralized bone matrix in human dermal fibroblasts in vitro. Cells Tissues Organs 180:151–158

    Article  PubMed  Google Scholar 

  • Nunes MC, Roy NS, Keyoung HM, Goodman RR, McKhann G III, Jiang L, Kang J, Nedergaard M, Goldman SA (2003) Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. Nat Med 9:439–447

    Article  PubMed  CAS  Google Scholar 

  • Toma JG, Akhavan M, Fernandes KJ, Barnabe-Heider F, Sadikot A, Kaplan DR, Miller FD (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784

    Article  PubMed  CAS  Google Scholar 

  • Tsonis PA, Goetinck PF (1990) Cell density dependent effect of a tumor promoter on proliferation and chondrogenesis of limb bud mesenchymal cells. Exp Cell Res 190:247–253

    Article  PubMed  CAS  Google Scholar 

  • Zhou G, Liu W, Cui L, Cao Y (2005) In vivo chondrogenesis of BMSCs at non-chondrogenesis site by co-transplantation of BMSCs and chondrocytes with pluronic as biomaterial. Key Eng Mater 288–289:3–6

    Article  Google Scholar 

  • Zhou G, Liu W, Cui L, Wang X, Liu T, Cao Y (2006) Repair of Porcine articular osteochondral defects in non-weightbearing areas with autologous bone marrow stromal cells. Tissue Eng 12:3209–3221

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Basic Research Program of China (2005CB522702), National Natural Science Foundation of China (30300353), and Hi-Tech Research and Development Program of China (2006AA02A126).

Author information

Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, 200011, Shanghai, China

    Xia Liu, Guangdong Zhou, Wei Liu, Wenjie Zhang, Lei Cui & Yilin Cao

Authors
  1. Xia Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guangdong Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wenjie Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lei Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yilin Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yilin Cao.

Additional information

Xia Liu and Guangdong Zhou contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, X., Zhou, G., Liu, W. et al. In vitro formation of lacuna structure by human dermal fibroblasts co-cultured with porcine chondrocytes on a 3D biodegradable scaffold. Biotechnol Lett 29, 1685–1690 (2007). https://doi.org/10.1007/s10529-007-9457-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-007-9457-8

Keywords

Search

Navigation