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Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules

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Abstract

Carrier induced granular particles comprising Enterobacter cloacae and Citrobacter freundii were used to generate H2 from sucrose in an anaerobic fluidized bed bioreactor. At a hydraulic retention time of 4.5 h, 95.8% of the sucrose was consumed and the rate of H2 production reached 180 mmol H2 l h−1. Biogas composition for H2 and CO2 was 42 and 55%, respectively.

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References

  • Angenent LT, Karim K, Al-Dahhan MH, Wrenn BA, Domíguez-Espinosa R (2004) Production of bioenergy and biochemical’s from industrial and agricultural wastewater. Trends Biotechnol 22:477–485

    Article  PubMed  CAS  Google Scholar 

  • Benemann J (1996) Hydrogen Biotechnology: Progress and prospects. Nat Biotechnol 14:1101–1103

    Article  PubMed  CAS  Google Scholar 

  • Chang F-Y, Lin C-Y (2004) Biohydrogen production using an upflow anaerobic sludge blanket reactor. Int J Hydrogen Energy 29:33–39

    Article  CAS  Google Scholar 

  • Endo G, Noike T, Matsumoto J (1982) Characteristics of cellulose and glucose decomposition in acidogenic phase of anaerobic digestion (in Japanese). Proc Soc Civ Eng 325:61–68

    Google Scholar 

  • Kerr PS, Huber SC, Israel DW (1984) Effect of N-source on soybean leaf sucrose phosphate synthase, starch formation, and whole plant growth. Plant Physiol 75:483–488

    Article  PubMed  CAS  Google Scholar 

  • Lee K-S, Lin P-J, Chang J-S (2005) Temperature effects on biohydrogen production in a granular sludge bed induced by activated carbon carrier. Int J Hydrogen Energy 27:1167–1174

    Google Scholar 

  • Levin DB, Pitt L, Love M (2004) Biohydrogen production: prospects and limitations to practical application. Int J Hydrogen Energy 29:173–185

    Article  CAS  Google Scholar 

  • Logan BE, Oh S-E, Kim IS, Van Ginkel S (2002) Biological hydrogen production measured in batch anaerobic respirometers. Environ Sci Technol 36:2530–2535

    Article  PubMed  CAS  Google Scholar 

  • Nandi R, Sengupta S (1998) Microbial production of hydrogen: an overview. Crit Rev Microbiol 24:61–84

    Article  PubMed  CAS  Google Scholar 

  • Schink B (1997) Energetics of syntrophic cooperation in methanogenic degradation. Microbiol Mol Biol Rev 61:262–280

    PubMed  CAS  Google Scholar 

  • Schnürer A, Zellner G, Svensson BH (1999) Mesophilic syntrophic acetate oxidation during methane formation in biogas reactors. FEMS Microbiol Ecol 29:249–261

    Google Scholar 

  • Thompson LJ, Gray V, Lindsay D, von Holy A (2006) Carbon: nitrogen: phosphorus ratios influence biofilm formation by Enterobacter cloacae and Citrobacter freundii. J Appl Microbiol 101:1105–1113

    Article  PubMed  CAS  Google Scholar 

  • Wu W-M, Jani MK, Conway de Macario E, Thiele JH, Zeikus JG (1992) Microbial composition and characterisation of prevalent methanogens and acetogens isolated from syntrophic methanogenic granules. Appl Microbiol Biotechnol 38:282–296

    Article  CAS  Google Scholar 

  • Wu S-Y, Lin C-N, Chang J-S (2003) Hydrogen production with immobilized sewage sludge in three-phase fluidized-bed bioreactors. Biotechnol Prog 19:828–832

    Article  CAS  Google Scholar 

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Acknowledgements

The bioreactor was built by Dave Amm. Basil Chassoulas provide invaluable assistance the electronics. Eskom TSI and the Council for Scientific and Industrial Research funded this project.

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Authors and Affiliations

  1. School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Wits, 2050, South Africa

    Liam Jed Thompson, Vincent Myles Gray, Bukasa Kalala, Denise Lindsay, Kelley Reynolds & Alex von Holy

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  1. Liam Jed Thompson
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  2. Vincent Myles Gray
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  3. Bukasa Kalala
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  4. Denise Lindsay
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  5. Kelley Reynolds
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  6. Alex von Holy
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Correspondence to Vincent Myles Gray.

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Alex von Holy—Deceased

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Thompson, L.J., Gray, V.M., Kalala, B. et al. Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules. Biotechnol Lett 30, 271–274 (2008). https://doi.org/10.1007/s10529-007-9527-y

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  • DOI: https://doi.org/10.1007/s10529-007-9527-y

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