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Biodegradation of organic compounds of molasses melanoidin (MM) from biomethanated distillery spent wash (BMDS) during the decolourisation by a potential bacterial consortium

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Abstract

Molasses melanoidin (MM) is a major pollutant in biomethanated distillery spent wash (BMDS) due to its recalcitrant properties. The 75% colour and 71% COD of MM (1,000 ppm) were reduced with developed bacterial consortium comprising Proteus mirabilis (IITRM5; FJ581028), Bacillus sp. (IITRM7; FJ581030), Raoultella planticola (IITRM15; GU329705) and Enterobacter sakazakii (IITRM16, FJ581031) in the ratio of 4:3:2:1 within 10 days at optimized nutrient. Bacterial consortium showed manganese peroxidase and laccase activity during MM decolourisation. The dominant growth of R. planticola and E. sakazakii was noted in consortium during MM decolourisation. The comparative GC–MS analysis of extracted compounds of control and degraded samples showed that most of the compounds present in control were completely utilized by bacterial consortium along with production of some metabolites. The developed bacterial consortium could be a tool for the decolourisation and degradation of melanoidin containing BMDS.

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Acknowledgments

The financial assistance from Council of Scientific Industrial Research, New Delhi to Ms. Sangeeta Yadav, SRF and project NWP–19 is duly acknowledged.

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  1. Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, 226025, Uttar Pradesh, India

    Sangeeta Yadav & Ram Chandra

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  1. Sangeeta Yadav
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  2. Ram Chandra
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Correspondence to Ram Chandra.

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Yadav, S., Chandra, R. Biodegradation of organic compounds of molasses melanoidin (MM) from biomethanated distillery spent wash (BMDS) during the decolourisation by a potential bacterial consortium. Biodegradation 23, 609–620 (2012). https://doi.org/10.1007/s10532-012-9537-x

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  • DOI: https://doi.org/10.1007/s10532-012-9537-x

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