biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:423-429, 2010 | DOI: 10.1007/s10535-010-0077-0

Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants

A. Das1, V. Kesari1, L. Rangan1,*
1 Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam, India

An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 μM benzyladenine and 2.68 μM α-naphthalene acetic acid (NAA) in C. longa or 2.46 μM indolebutyric acid in C. amada. Effect of sugars and agar at different concentrations were also studied and 2 % maltose and 0.7 % agar were found optimum for shoot multiplication and regeneration. Most plantlets developed roots simultaneously but others formed roots when subcultured in 1/2 MS medium supplemented with 2.68 μM NAA. Plants were successfully hardened in greenhouse with 80 % survival. The genetic purity of micropropagated plantlets was analyzed using RAPD and protein profiles.

Keywords: axillary bud; micropropagation; RAPD; SDS-PAGE; Zingiberaceae
Subjects: auxins; Curcuma amada; Curcuma longa; cytokinins; genetic stability; in vitro culture, direct organogenesis; in vitro culture, rooting; random amplified polymorphic DNA (RAPD)

Received: December 16, 2008; Accepted: June 11, 2009; Published: September 1, 2010  Show citation

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Das, A., Kesari, V., & Rangan, L. (2010). Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants. Biologia plantarum, 54(3), 423-429. doi: 10.1007/s10535-010-0077-0
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