Abstract
Secretory proteins encoded by genes expressed in the pharyngeal gland cells of plant-parasitic nematodes have key roles in nematode parasitism of plants. A new β-1,4-endoglucanase gene (designated Ha-eng-1a) was isolated from the cereal cyst nematode Heterodera avenae. The cDNA of Ha-eng-1a encoded a deduced 463-amino acid sequence containing a catalytic domain and a cellulose binding module separated by a linker. The genomic DNA of Ha-eng-1a is 2,129-bp long, containing eight introns ranging from 56 bp to157 bp and nine exons ranging from 70 bp to 299 bp. Southern blot analysis revealed that the Ha-eng-1a gene has two copies. In situ hybridization showed that the Ha-eng-1a transcriptsspecifically accumulated in the two subventral gland cells of the second-stage juveniles. There was evidence for cellulase activity of the recombinant protein Ha-eng-1a in vitro. The results indicated that this β-1,4-endoglucanase gene may play a crucial role in plant cell wall-degradation during penetration and migration of nematodes in the host roots.
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Acknowledgements
This research was supported by Nature Sciences Foundation of China (30921140411) and the China Ministry of Science and Technology (2009DFB30230). Profs Zhou Guanghe and Tang Wenhua,Cynthia Eden (M.A), University of Guelph are thanked for their scientific advices and assistances.
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Long, H., Peng, H., Huang, W. et al. Identification and molecular characterization of a new β-1,4-endoglucanase gene (Ha-eng-1a) in the cereal cyst nematode Heterodera avenae . Eur J Plant Pathol 134, 391–400 (2012). https://doi.org/10.1007/s10658-012-9997-1
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DOI: https://doi.org/10.1007/s10658-012-9997-1