Abstract
Poly(3,4-ethylenedioxythiophene) (PEDOT) was potentiostatically polymerized onto gold electrode with adenosine 5′-triphosphate (ATP), an important biomolecule, as the counterion. Essential parameters to affect the impedance of the resultant polymer were studied in detail. Results show that 1.0 V, 0.1 M and 2.2 mC is the optimal deposition potential, dopant concentration and quantity of the passing charges, respectively. Surface topography was studied by AFM and the relationship between impedance and topography was discussed. Spontaneous release of ATP from PEDOT matrix was examined using UV-visible spectroscopy and impedance variation of the polymer modified electrode was monitored with electrochemical impedance spectroscopy. The results indicate that ATP could be strongly bound with the polymer in the incubation medium and thus could realize its role as a cell binder in its implantable application. In vitro test further demonstrates that PC12 cell could adhere to and grow on the PEDOT/ATP modified electrode and cell attachment percentage was much higher than that of non-biomolecule doped PEDOT modified electrode. Moreover, stability of PEDOT/ATP in the biological reducing agent of glutathione (GSH) was much better than that of polypyrrole/ATP. Our work demonstrates that PEDOT/ATP modified electrode can be of a promising bioactive platform to be used for implantable neural recording devices due to its great stability and biocompatibility.
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References
Webb K, Budko E, Neuberger TJ, Chen S, Schachner M, Tresco PA (2001) Biomaterials 22:1017–1028
Xiao YH, Cui XY, Hancock JM, Bouguettaya M, Reynolds JR, Martin DC (2004) Sens Actuators B 99:437–443
Moxon KA, Leiser SC, Gerhardt GA, Barbe KA, Chapin JK (2004) IEEE Trans Biomed Eng 51:647–656
Hoogerwerf AC, Wise KD (1994) IEEE Trans Biomed Eng 41:1136–1146
Edell DJ, Toi VV, McNeil VM, Clark LD (1992) IEEE Trans Biomed Eng 39:635–643
Aylward WM, Pickup PG (2007) Electrochim Acta 52:6275–6281
Cui X, Li CM, Zang J, Zhou Q, Gan Y, Bao H, Guo J, Lee VS, Moochhala SM (2007) J Phys Chem C 111:2025–2031
Xiao YH, Che JF, Li CM, Sun CQ, Chua YT, Lee VS, Luong JHT (2007) J Biomed Mater Res 80A:925–931
Berdichevsky Y, Lo Y-H (2006) Adv Mater 18:122–125
Drillet J-F, Dittmeyer R, Juttner K (2007) J Appl Electrochem 37:1219–1226
Rumbau V, Pomposo JA, Eleta A, Rodriguez J, Grande H, Mecerreyes D, Ochoteco E (2007) Biomacromolecules 8:315–328
Xiao YH, Cui X, Martin DC (2004) J Electroanal Chem 573:43–48
Jang J, Chang M, Yoon H (2005) Adv Mater 17:1616–1620
Yamato H, Ohwa M, Wernet W (1995) J Electroanal Chem 397:163–170
Heywang G, Jonas F (1992) Adv Mater 4:116–118
Groenendaal LB, Jonas F, Freitag D, Pielartzik H, Reynolds JR (2000) Adv Mater 12:481–494
Xiao YH, Li CM, Yu SC, Zhou Q, Lee VS, Moochhala SM (2007) Talanta 72:532–538
Wallace GG, Kane-Maguire LAP (2002) Adv Mater 14:953–960
Richardson RT, Thompson B, Moulton S, Newbold C, Lum MG, Cameron A, Wallace GG, Kapsa R, Clark G, O’Leary S (2007) Biomaterials 28:513–523
Kamei J, Takahashi Y, Yoshikawa Y, Saitoh A (2005) Eur J Pharm 528:158–161
Siow NL, Xie HQ, Choi RCY, Tsima KWK (2005) Chem Biol Interact 157–158:423–426
Matsumoto N, Sorimachi M, Akaike N (2004) Brain Res 1009:234–237
Sakmeche N, Aeiyach S, Aaron J-J, Jouini M, Lacroix JC, Lacaze P-C (1999) Langmuir 15:2566–2572
Pernaut J-M, Reynolds JR (2000) J Phys Chem B 104:4080–4090
Li CM, Sun CQ, Song S, Choong VE, Maracas G, Zhang XJ (2005) Front Biosci 10:180–186
Li CM, Chen W, Yang X, Sun CQ, Gao C, Zheng ZX, Sawyer J (2005) Front Biosci 10:2518–2526
Chen W, Li CM, Chen P, Sun CQ (2006) Electrochim Acta 52:1082–1086
Skotheim TA, Reynolds J (1998) Handbook of conducting polymers, 2nd edn. Marcel Dekker, New York
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The work is financially supported by the Center for Advanced Bionanosystem, Nanyang Technological University.
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Xiao, Y.H., Li, C.M., Toh, ML. et al. Adenosine 5′-triphosphate incorporated poly(3,4-ethylenedioxythiophene) modified electrode: a bioactive platform with electroactivity, stability and biocompatibility. J Appl Electrochem 38, 1735–1741 (2008). https://doi.org/10.1007/s10800-008-9623-2
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DOI: https://doi.org/10.1007/s10800-008-9623-2