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Effects of alkylated-chitosan–DNA nanoparticles on the function of macrophages

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Abstract

Chitosan could form nanoparticles with DNA through electrostatic interaction, and hence protect the DNA from enzymatic degradation. Numerous studies have been working on modifying chitosan aiming at improving its transgenic efficacy. While the modification of chitosan with alkyl group has been shown to significantly improve the cell transfection efficiency, little is known about its impact on its biocompatibility. The current study was performed to investigate the impact of alkylated-chitosan/DNA nanoparticles on the function of the murine macrophage through observing its phagocytic activity and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-12 and TNF-α). Our results demonstrated that the alkylated-chitosan/DNA nanoparticles at the concentration of 20 μg/ml DNA content had no significant impact on the production of cytokines and phagocytic activity of the macrophages as compared with the unmodified chitosan/DNA nanoparticles and negative control even after 24 h co-incubation. It suggested that the modification of chitosan with alkyl group should not have negative impact on the function of the macrophages.

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Acknowledgements

This research was jointly supported by the Ministry of Science and Technology of China (Grant No: 2005DIB1J094, 2006CB933203) and the National Natural Science Foundation of China (Grant No: 90406024).

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Authors and Affiliations

  1. Tianjin Key Laboratory of Biomedical materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China

    L. X. Liu, C. N. Song, L. P. Song, H. L. Zhang, X. Dong & X. G. Leng

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  1. L. X. Liu
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  2. C. N. Song
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  3. L. P. Song
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  4. H. L. Zhang
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  6. X. G. Leng
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Correspondence to X. G. Leng.

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Liu, L.X., Song, C.N., Song, L.P. et al. Effects of alkylated-chitosan–DNA nanoparticles on the function of macrophages. J Mater Sci: Mater Med 20, 943–948 (2009). https://doi.org/10.1007/s10856-008-3621-0

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  • DOI: https://doi.org/10.1007/s10856-008-3621-0

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