Abstract
High corrosion rate and accumulation of hydrogen gas upon degradation impede magnesium alloys’ clinical application as implants. In this work, micro-arc oxidation (MAO) was used to fabricate a porous coating on magnesium alloys as an intermediate layer to enhance the bonding strength of propolis layer. Then the composite coatings were fabricated using sol–gel method by dipping sample into the solution containing propolis and polylactic acid at 40°C. The corrosion resistance of the samples was determined based on potentiodynamic polarization experiments and immersion tests. Biocompatibility was designed by observing the attachment and growth of wharton’s jelly-derived mesenchymal stem cells (WJCs) on substrates with MAO coating and substrates with composite coatings. The results showed that, compared with that of Mg–Zn–Ca alloy, the corrosion current density of the samples with composite coatings decreased from 5.37 × 10−5 to 1.10 × 10−6 A/cm2 and the corrosion potential increased by 240 mV. Composite coatings exhibit homogeneous corrosion behavior and can promote WJCs cell adhesion and proliferation. In the meantime, pH value was relatively stable during the immersion tests, which may be significant for cellular survival. In conclusion, our results indicate that composite coatings on Mg–Zn–Ca alloy fabricated by MAO/sol–gel method provide a new type bioactive material.
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References
Witte F, Fischer J, Nellesen J, Vogt C, Vogt J, Donath T, Beckmann F. In vivo corrosion and corrosion protection of magnesium alloy LAE442. Acta Biomater. 2010;6:1792–9.
Staiger MP, Pietak AM, Huadmai J, Dias G. Magnesium and its alloys as orthopedic biomaterials: a review. Biomaterials. 2006;27:1728–34.
Zheng YF, Gu XN, Xi YL, Chai DL. In vitro biocorrosion and cytotoxicity of Mg/Ca composite. Acta Biomater. 2010;6:1783–91.
Wang J, Wang LG, Gua SK, Zhu SJ, Ren CX, Hou SS. Microstructure and corrosion properties of as sub-rapid solidification Mg–Zn–Y–Nd alloy in dynamic simulated body fluid for vascular stent application. J Mater Sci Mater Med. 2010;21:2001–8.
Li JN, Song Y, Zhang SX, Zhao CZ, Zhang F, Zhang XN, Cao L, Fa QM, Tang TT. In vitro response of human bone marrow stromal cells to a fluoridated hydroxyapatite coated biodegradable Mg–Zn alloy. Biomaterials. 2010;31:5782–8.
Xu LP, Pan F, Yu GN, Yang L, Zhang EL, Yang K. In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy. Biomaterials. 2009;30:1512–23.
Gao JH, Guan SK, Chen J, Wang LG, Zhu SJ, Hu JH, Ren ZW. Fabrication and characterization of rod-like nano-hydroxyapatite on MAO coating supported on Mg–Zn–Ca alloy. Appl Surf Sci. 2011;257:2231–7.
Wen CL, Guan SK, Peng L, Ren CX, Wang X, Hu ZH. Characterization and degradation behavior of AZ31 alloy surface modified by bone-like hydroxyapatite for implant applications. Appl Surf Sci. 2009;255:6433–8.
Vohra S, Hennessy K, Sawyer A, Zhuo Y, Bellis S. Comparison of mesenchymal stem cell and osteosarcoma cell adhesion to hydroxyapatite. J Mater Sci Mater Med. 2008;19:3567–74.
NNIS System. NNIS System report,data summary from january 1992 through June 2004. Am J Infect Control. 2004;32:470.
Silici S, Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol. 2005;99:69–73.
Can A, Karahuseyinoglu S. Human umbilical cord stroma with regard to the source of fetus-derived stem cells. Stem Cells. 2007;25:2886–95.
Troyer DL, Weiss ML. Wharton’s jelly-derived cells are a primitive stromal cell population. Stem Cells. 2008;26:591–9.
Gao JH, Guan SK, Ren ZW, Sun YF, Zhu SJ, Wang B. Homogeneous corrosion of high pressure torsion treated Mg–Zn–Ca alloy in simulated body fluid. Mate Lett. 2011;65:691–3.
Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials. 2006;27:2907–15.
Zhao LC, Cui CX, Wang QZ, Bu SJ. Growth characteristics and corrosion resistance of micro-arc oxidation coating on pure magnesium for biomedical applications. Corros Sci. 2010;52:2228–34.
Liang J, Hu LT, Hao JC. Improvement of corrosion properties of micro arc oxidation coating on magnesium alloy by optimizing current density parameters. Appl Surf Sci. 2007;253:6939–45.
Stern M, Geary AL. Electrochemical polarization, No.1: theoretical analysis of the shape of polarization curves. J Electrochem Soc. 1957;104:56–63.
Su P, Wu XH, Guo Y, Jiang ZH. Effects of cathode current density on structure and corrosion resistance of plasma electrolytic oxidation coatings formed on ZK60 Mg alloy. J Alloy Comp. 2009;475:773–7.
Tan ALK, Soutar AM, Annergren IF, Liu YN. Multilayer sol–gel coatings for corrosion protection of magnesium. Surf Coat Technol. 2005;198:478–82.
Meng EC, Guan SK, Wang HX, Wang LG, Zhu SJ, Hu JH, Ren CX, Gao JH, Feng YS. Appl Surf Sci. doi: 10.1016/j.apsusc.2010.12.073.
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The authors are grateful for the financial support of the National Natural Science Foundation of China (No. 30870634) and the National Basic Research Program of China (Grant No. 2008CB617509).
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J.H. Gao and X.Y. Shi made equal contributions to this work.
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Gao, J.H., Shi, X.Y., Yang, B. et al. Fabrication and characterization of bioactive composite coatings on Mg–Zn–Ca alloy by MAO/sol–gel. J Mater Sci: Mater Med 22, 1681–1687 (2011). https://doi.org/10.1007/s10856-011-4349-9
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DOI: https://doi.org/10.1007/s10856-011-4349-9