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Single Step Fabrication of Chitosan Nanocrystals Using Penaeus semisulcatus: Potential as New Insecticides, Antimicrobials and Plant Growth Promoters

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Abstract

In this study, chitosan nanoparticles (CH-NPs) were synthesized using Penaeus semisulcatus shrimp shells and characterized using UV–Vis and FT-IR spectroscopy, as well as XRD and HR-TEM analyses. CH-NPs were investigated for growth inhibition properties against selected species of bacterial and fungal pathogens, showing performances higher or comparable over positive controls, respectively. Furthermore, CH-NPs were tested on three important mosquito vectors, achieving LC50 from 12.27 to 14.62 µg/ml. In addition, CH-NPs were evaluated using in vitro plant tissue culture by rooting gel method, to enhance the vegetative growth of the medicinal plant species Sphaeranthus indicus. With the simple technique presented here, large-scale industrial production of CH-NPs is possible. They can be used to develop pesticides highly effective against mosquito vectors of high medical and veterinary importance, as well as for plant tissue culture and food packaging applications.

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Acknowledgements

The authors extend their sincere appreciations to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. (RGP-073). This research was supported by Alagappa University Research Fund (AURF), Karaikudi, for Minor Research Project (MRP-2017). One of the authors (M.B.) is highly grateful to the School of Physics, and Department of Botany, Alagappa University, for extending XRD and greenhouse facility. We express our gratitude to G. Rajkumar (Department of Zoology, Bharathiar University, Coimbatore) for providing the P. semisulcatus shells. We are indebted to KRIND Institute of Research and Development, Trichy, for their assistance in analysis of antibacterial and antifungal activities.

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Authors and Affiliations

  1. Department of Industrial Chemistry, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Vijayan Thamilarasan

  2. College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314 001, China

    Vijayan Thamilarasan

  3. Department of Chemistry, Vel Tech Multi Tech, Avadi, Chennai, Tamil Nadu, 600 062, India

    Venkatesan Sethuraman

  4. Department of Packaging, Yonsei University, Yonseidae-gil, Wonju, Gangwondo, 220 710, Republic of Korea

    Kasi Gopinath

  5. Department of Nanoscience and Technology, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Chinnasamy Balalakshmi

  6. Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

    Marimuthu Govindarajan

  7. Government College for Women, Kumbakonam, Tamil Nadu, 602 001, India

    Marimuthu Govindarajan

  8. Department of Pharmacognosy, College of Pharmacy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Ramzi A. Mothana & Nasir A. Siddiqui

  9. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Jamal M. Khaled

  10. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

  11. The BioRobotics Institute, Sant’Anna School of Advanced Studies, Viale Rinaldo Piaggio 34, Pontedera, 56025, Pisa, Italy

    Giovanni Benelli

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  1. Vijayan Thamilarasan
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  2. Venkatesan Sethuraman
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  3. Kasi Gopinath
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Correspondence to Kasi Gopinath or Chinnasamy Balalakshmi.

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Thamilarasan, V., Sethuraman, V., Gopinath, K. et al. Single Step Fabrication of Chitosan Nanocrystals Using Penaeus semisulcatus: Potential as New Insecticides, Antimicrobials and Plant Growth Promoters. J Clust Sci 29, 375–384 (2018). https://doi.org/10.1007/s10876-018-1342-1

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