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Spectrofluorimetric Determination of Second Critical Micellar Concentration of SDS and SDS/Brij 30 Systems

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Abstract

Potentially useful stead-state fluorimetric technique was used to determine the critical micellar concentrations (CMC1 and CMC2) for two micellar media, one formed by SDS and the other by SDS/Brij 30. A comparative study based on conductimetric and surfacial tension measurements suggests that the CMC1 estimated by the fluorimetric method is lower than the value estimated by these other techniques. Equivalent values were observed for SDS micelles without Brij 30 neutral co-surfactant. The use of acridine orange as fluorescent probe permitted to determine both CMC1 and CMC2. Based on it an explanation on aspects of micelle formation mechanism is presented, particularly based on a spherical and a rod like structures.

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Acknowledgments

Financial support from FAPESP (06/56701-3; 02/00272-6) and CNPq are gratefully acknowledged. H.P.M.O. thanks to Vinicius C. C. Vieira for the help, discussion and suggestions in this work.

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Authors and Affiliations

  1. Departamento de Química–FFCLRP, Universidade de São Paulo, Ribeirão Preto, Brazil

    Ana Paula Romani

  2. LAFOT–Instituto de Química, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil

    Antonio Eduardo da Hora Machado

  3. Departamento de Química, Univeridade Estadual de Maringá, Maringá, Paraná, Brazil

    Noboru Hioka

  4. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil

    Divinomar Severino & Mauricio S. Baptista

  5. Instituto de Pesquisa e Desenvolvimento—IP&D, Universidade do Vale do Paraíba—UNIVAP, Av. Shishima Hifumi 2911, Urbanova, CEP: 12244-000, São José dos Campos, SP, Brazil

    Lúcia Codognoto, Maira R. Rodrigues & Hueder Paulo Moisés de Oliveira

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  1. Ana Paula Romani
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  2. Antonio Eduardo da Hora Machado
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Correspondence to Hueder Paulo Moisés de Oliveira.

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Romani, A.P., da Hora Machado, A.E., Hioka, N. et al. Spectrofluorimetric Determination of Second Critical Micellar Concentration of SDS and SDS/Brij 30 Systems. J Fluoresc 19, 327–332 (2009). https://doi.org/10.1007/s10895-008-0420-4

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  • DOI: https://doi.org/10.1007/s10895-008-0420-4

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