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Electrospun PLLA nanofiber scaffolds for bladder smooth muscle reconstruction

  • Urology - Original Paper
  • Published:
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Abstract

Purpose

Urinary bladder may encounter several pathologic conditions that could lead to loss of its function. Tissue engineering using electrospun PLLA scaffolds is a promising approach to reconstructing or replacing the problematic bladder.

Methods

PLLA nanofibrous scaffolds were prepared utilizing single-nozzle electrospinning. The morphology and distribution of fiber diameters were investigated by scanning electron microscopy (SEM). Human bladder smooth muscle cells (hBSMCs) were isolated from biopsies and characterized by immunocytochemistry (ICC). Then, the cells were seeded on the PLLA nanofibers and Alamar Blue assay proved the biocompatibility of prepared scaffolds. Cell attachment on the nanofibers and also cell morphology over fibrous scaffolds were observed by SEM.

Results

The results indicated that electrospun PLLA scaffold provides proper conditions for hBSMCs to interact and attach efficiently to the fibers. Alamar Blue assay showed the compatibility of the obtained electrospun scaffolds with hBSMCs. Also, it was observed that the cells could achieve highly elongated morphology and their native aligned direction besides each other on the random electrospun scaffolds and in the absence of supporting aligned nanofibers.

Conclusion

Electrospun PLLA scaffold efficiently supports the hBSMCs growth and alignment and also has proper cell compatibility. This scaffold would be promising in urinary bladder tissue engineering.

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Acknowledgments

The authors would like to express their special gratitude to Dr. Shahram Gooran from TUMS Urology Center at Sina Hospital.

Funding

This research has been supported by Tehran University of Medical Sciences and Health Services (Grant No. 92-02-87-22110).

Author information

Authors and Affiliations

  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 1417755469, Tehran, Iran

    Mohammad Ali Derakhshan, Gholamreza Pourmand, Hossein Ghanbari & Reza Faridi-Majidi

  2. Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Gholamreza Pourmand

  3. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Rassoul Dinarvand

  4. Urology and Nephrology Research Center (UNRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Naji

  5. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Jafar Ai

Authors
  1. Mohammad Ali Derakhshan
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  2. Gholamreza Pourmand
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  3. Jafar Ai
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  4. Hossein Ghanbari
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  5. Rassoul Dinarvand
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  6. Mohammad Naji
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  7. Reza Faridi-Majidi
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Corresponding author

Correspondence to Reza Faridi-Majidi.

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The authors declare that they have no conflict of interest.

Ethical approval

The procedures involving human participants were performed in accordance with the ethical approval of Tehran University of Medical Sciences and with the 1964 Declaration of Helsinki and subsequent amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Derakhshan, M.A., Pourmand, G., Ai, J. et al. Electrospun PLLA nanofiber scaffolds for bladder smooth muscle reconstruction. Int Urol Nephrol 48, 1097–1104 (2016). https://doi.org/10.1007/s11255-016-1259-2

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  • DOI: https://doi.org/10.1007/s11255-016-1259-2

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