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The pattern of toxin genes and expression of diarrheal enterotoxins in Bacillus thuringiensis strains isolated from commercial bioinsecticides

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Abbreviations

Bt :

Bacillus thuringiensis

Bc :

Bacillus cereus

DSMZ:

deutsche sammlung von mikroorganismen und zellkulturen

EFSA:

European food safety authority

ELISA:

enzyme-linked immunosorbent assay

HBL:

haemolysin BL

NHE:

non-haemolytic enterotoxin

RPLA:

reverse passive latex agglutination

QPS:

qualified presumption of safety

References

  • Agata N., Ohta M., Mori M., Isobe M., 1995. A novel dodecadepsipeptide, cereulide, is an emetic toxin of Bacillus cereus. FEMS microbiology letters, 129(1), 17–20.

    PubMed  CAS  Google Scholar 

  • Ash C. and Collins M.D., 1992. Comparative analysis of 23S ribosomal RNA gene sequences of Bacillus anthracis and emetic Bacillus cereus determined by PCR-direct sequencing. FEMS microbiology letters, 94, 75–80.

    Article  CAS  Google Scholar 

  • Carlson C.R., Johansen T., Kolsto A.B., 1996. The chromosome map of Bacillus thuringiensis subsp. canadensis HD244 is highly similar to that of Bacillus cereus type strain ATCC 14579. FEMS microbiology letters, 141, 163–167.

    Article  PubMed  CAS  Google Scholar 

  • Decreto Legislativo n. 194, 1995, Attuazione della direttiva 91/414/CEE in materia di immissione in commercio di prodotti fitosanitari. Gazzetta Ufficiale n. 122 Supplemento Ordinario del 27/05/1995.

  • EFSA, 2007. Introduction of a Qualified Presumption of Safety (QPS) Approach for Assessment of Selected Microorganisms Referred to EFSA - Opinion of the Scientific Committee - (EFSA-Q-2005-293) - (Adopted on 19 November 2007). EFSA Journal, 587, 1–16.

    Google Scholar 

  • Ehling-Schulz M., Vukov N., Schulz A., Shaheen R., Andersson M., Märtlbauer E., Scherer S., 2005. Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus. Applied and Environmental Microbiology, 71(1), 105–713.

    Article  PubMed  CAS  Google Scholar 

  • Hansen B.M. and Hendriksen N.B., 2001. Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis. Applied and Environmental Microbiology, 67, 185–189.

    Article  PubMed  CAS  Google Scholar 

  • Kyei-Poku G., Gauthier D., Pang A., van Frankenhuyzen K., 2007. Detection of Bacillus cereus virulence factors in commercial products of Bacillus thuringiensis and expression of diarrheal enterotoxins in a target insect. Canadian Journal of Microbiology, 53(12) 1283–1290.

    Article  PubMed  CAS  Google Scholar 

  • Lund T., De Buyser M.L., Granum P.E., 2000. A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. Molecular Microbiology, 38(2), 254–261.

    Article  PubMed  CAS  Google Scholar 

  • Manzano M., Cocolin L., Cantoni C., Comi G., 1998. A rapid method for the identification and partial serotyping of Listeria monocytogenes in food by PCR and restriction enzyme analysis. International Journal of Food Microbiology, 42(3), 207–212.

    Article  PubMed  CAS  Google Scholar 

  • Murray P.R., Baron E.J., Pfaller M.A., Tenover F.C., Yolken R.H., 1999. Manual of clinical microbiology, 7th edition. ASM Press: Washington.

    Google Scholar 

  • Schnepf E., Crickmore N., Van R.J., Lereclus D., Baum J., Feitelson J., Zeigler D.R., Dean D.H., 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews, 62(3), 775–806.

    PubMed  CAS  Google Scholar 

  • Schoeni J.L. and Wong A.C.L., 2005. Bacillus cereus food poisoning and its toxins. Journal of Food Protection, 68, 636–648.

    PubMed  CAS  Google Scholar 

  • Schraft H. and Griffiths M.W., 1995. Specific oligonucleotide primers for detection of lecithinase-positive Bacillus spp. by PCR. Applied and Environmental Microbiology, 61(1), 98–102.

    PubMed  CAS  Google Scholar 

  • Seligy V.L., Beggs R.W., Rancourt J.M., Tayabali A.F., 1997. Quantitative bioreduction assays for calibrating spore content and viability of commercial Bacillus thuringiensis insecticides. Journal of Industrial Microbiology and Biotechnology, 18, 370–378.

    Article  CAS  Google Scholar 

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Acknowledgements

The research was carried out with the help of funds from the Fondazione Banco di Sardegna

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Correspondence to C. Scarano.

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Scarano, C., Virdis, S., Cossu, F. et al. The pattern of toxin genes and expression of diarrheal enterotoxins in Bacillus thuringiensis strains isolated from commercial bioinsecticides. Vet Res Commun 33 (Suppl 1), 257–260 (2009). https://doi.org/10.1007/s11259-009-9288-2

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