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Evidences of biological control capacities of Streptomyces spp. against Sclerotium rolfsii responsible for damping-off disease in sugar beet (Beta vulgaris L.)

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Abstract

Ten antibiotic-producing Streptomyces spp. isolated from Moroccan soils were evaluated for their ability to inhibit in vitro Sclerotium rolfsii development. Four isolates having the greatest pathogen inhibitory capabilities were subsequently tested for their ability to inhibit sclerotial germination in sterile soil. This test was carried out by using biomass inoculum, culture filtrate, and spore suspension of the isolates as treatment. Treatment with biomass inoculum and culture filtrate gave the highest inhibition of sclerotia. Biological control tests against Sclerotium rolfsii damping-off of sugar beet seeds showed that the selected Streptomyces isolates reduced significantly the disease severity, the J-2 isolate being the more potent. In addition, treatment with the isolate J-2 resulted in a significant increase (P ≤ 0.05) in seedling development compared to the control. All antagonistic Streptomyces selected here were able to grow in the rhizosphere soil from infected sugar beet culture.

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Acknowledgements

We are grateful to Pr. T. Kawano for critical reading of our paper. This work was supported by funds from the AI MA/04/110F, from PRAD 04/02 and AUF 63-01PS615.

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Authors and Affiliations

  1. Département de Biologie, Faculté des Sciences-Semlalia, Laboratoire de Biologie et Biotechnologie des Microorganismes (LBBM), Equipe d’Ecologie et Biotechnologie Microbienne, B.P. 2390, Marrakesh, 40000, Morocco

    Rafik Errakhi & Mustapha Barakate

  2. Laboratoire d’Electrophysiologie des Membranes, EA3514, Université Paris Diderot, 2 place Jussieu, Paris cedex 05, 750251, France

    Rafik Errakhi & Francois Bouteau

  3. Laboratoire de Génie Chimique, Institut National Polytechnique de Toulouse ENSAT, 1 Avenue de l’Agrobiopole, Auzeville-Tolosane, 31326, France

    Rafik Errakhi & Ahmed Lebrihi

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  1. Rafik Errakhi
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  2. Francois Bouteau
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  3. Ahmed Lebrihi
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  4. Mustapha Barakate
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Correspondence to Mustapha Barakate.

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Errakhi, R., Bouteau, F., Lebrihi, A. et al. Evidences of biological control capacities of Streptomyces spp. against Sclerotium rolfsii responsible for damping-off disease in sugar beet (Beta vulgaris L.). World J Microbiol Biotechnol 23, 1503–1509 (2007). https://doi.org/10.1007/s11274-007-9394-7

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  • DOI: https://doi.org/10.1007/s11274-007-9394-7

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