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A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment

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Abstract

Purpose

Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging.

Procedures

We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models.

Results

The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment.

Conclusions

This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.

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Acknowledgements

We thank Dr. Mark Gaon for help developing and testing the gated anesthesia instrument. Some of this work was done in partial fulfillment of Ph.D. thesis research requirements by Dr. J.Y. Hwang, at the University of Southern California. Work at the California Institute of Technology was supported by the Arnold and Mabel Beckman Foundation. Z.G. thanks Johnson & Johnson for research support. We are grateful for the following federal support of our research: NIH (5R01CA123495-03 and 1U01CA151815-0) to JYL; NIH (1R01 CA140995 and 1R01 CA129822) and DOD W81XWH-06-1-0549 to LKMK; and US Navy Bureau of Medicine and Surgery (1435-04-04-GT-41387 and -43096), NIH (N01-CO-07119), and NSF (BESOO 79483) to DLF.

Conflict of Interest. The authors declare they have no conflicts of interest.

Author information

Author notes

  1. Daniel L. Farkas

    Present address: Spectral Molecular Imaging, Inc, Beverly Hills, CA, 90211, USA

Authors and Affiliations

  1. Minimally Invasive Surgical Technologies Institute and Department of Surgery, Cedars-Sinai Medical Center, 8700 Beverly Blvd. D6061, Los Angeles, CA, 90048, USA

    Jae Youn Hwang, V. Krishnan Ramanujan & Daniel L. Farkas

  2. Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, 90089, USA

    Jae Youn Hwang & Daniel L. Farkas

  3. Department of Biomedical Sciences, Cedars-Sinai Medical Center, 8700 Beverly Blvd. D6061, Los Angeles, CA, 90048, USA

    Jae Youn Hwang, V. Krishnan Ramanujan, Lali K. Medina-Kauwe & Daniel L. Farkas

  4. NSF Center for Biophotonics, Science and Technology and Department of Pathology and Laboratory Medicine, University of California Davis, 2700 Stockton Blvd., Suite 1400, Sacramento, CA, 95817, USA

    Sebastian Wachsmann-Hogiu

  5. Department of Neurosugery, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Davis 2096, Los Angeles, CA, 90048, USA

    Julia Ljubimova

  6. Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa, 32000, Israel

    Zeev Gross

  7. Department of Chemistry and the Beckman Institute, California Institute of Technology, Pasadena, CA, 91125, USA

    Harry B. Gray & Daniel L. Farkas

  8. David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Lali K. Medina-Kauwe

Authors
  1. Jae Youn Hwang
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  8. Daniel L. Farkas
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Corresponding author

Correspondence to Daniel L. Farkas.

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Hwang, J.Y., Wachsmann-Hogiu, S., Ramanujan, V.K. et al. A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment. Mol Imaging Biol 14, 431–442 (2012). https://doi.org/10.1007/s11307-011-0517-z

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  • DOI: https://doi.org/10.1007/s11307-011-0517-z

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