Abstract
Microencapsulation of bixin using high-amylose corn starch was carried out by the acidification method. Bixin powders were characterized by differential scanning calorimetry (DSC), X-ray diffractometry (XRD), FT-IR spectrometry, color parameters, encapsulation efficiency, bixin release profile. In addition, the effect of whey protein (WP) on the microencapsulation process was investigated. The results obtained from DSC, X-ray diffraction and FT-IR spectrometry indicated that only in the samples prepared at 90 °C (B0WP90°C, B10WP90°C, and B20WP90°C) there was formation of crystalline structures, with melting temperatures at 117.2°, 105° and 104 °C, respectively. The possible interactions between bixin, WP and amylose starch are also discussed.
Similar content being viewed by others
References
R.E. Wrolstad, C.A. Culver, Alternatives to those Artificial FD&C Food Colorants. Annu. Rev. Food Sci. Technol. 3, 59–77 (2012)
P. Giridhar, A. Venugopalan, R.A. Parimalan, Review on annatto dye extraction , analysis and processing – A food technology perspective. JSRR. 3, 327–348 (2014)
C.R. Cardarelli, Benassi M de T, Mercadante AZ. Characterization of different annatto extracts based on antioxidant and colour properties. LWT Food Sci. Technol. 41, 1689–1693 (2008)
M. Abayomi, A.S. Adebayo, D. Bennett, R. Porter, J. Shelly-Campbell, In vitro antioxidant activity of Bixa orellana (annatto) seed extract. JAPS. 4, 101–106 (2014)
A.D.O. Rios, L.M.G. Antunes, L.P. Bianchi M de, Proteção de carotenóides contra radicais livres gerados no tratamento de câncer com cisplatina. Alim. Nutr. 20, 343–350 (2009)
T. Taham, F.A. Cabral, M.A.S. Barrozo, Extraction of bixin from annatto seeds using combined technologies. J. Supercrit. Fluids 100, 175–183 (2015)
K. Balaswamy, P.G. Prabhakara Rao, A. Satyanarayana, D.G. Rao, Stability of bixin in annatto oleoresin and dye powder during storage. LWT Food Sci. Technol. 39, 952–956 (2006)
A.L. Parize, T. Cristina, S.R. De, Maria I, Brighente C, Fávere VT De, et al. microencapsulation of the natural urucum pigment with chitosan by spray drying in different solvents. Afr. J. Biotechnol. 7, 3107–3114 (2008)
M.I.M. Barbosa, C. Borsarelli, A. Mercadante, Light stability of spray-dried bixin encapsulated with different edible polysaccharide preparations. Food Res. Int. 38, 989–994 (2005)
K.B. De Sousa Lobato, K. Paese, J. Casanova, S. Stanisçuaski, A. Jablonski, A. De Oliveira, Characterisation and stability evaluation of bixin nanocapsules. Food Chem. 141, 3906–3912 (2013)
S.M. Lyng, M. Passos, J.D. Fontana, Bixin and α-cyclodextrin inclusion complex and stability tests. Process Biochem. 40, 865–872 (2005)
V.A. Marcolino, G.M. Zanin, L.R. Durrant, M.D.T. Benassi, G. Matioli, Interaction of curcumin and bixin with β-cyclodextrin: Complexation methods, stability, and applications in food. J. Agric. Food Chem. 59, 3348–3357 (2011)
T. Itthisoponkul, J.R. Mitchell, A.J. Taylor, Farhat I a. Inclusion complexes of tapioca starch with flavour compounds. Carbohydr. Polym. 69, 106–115 (2007)
I. Lalush, H. Bar, I. Zakaria, S. Eichler, E. Shimoni, Utilization of amylose-lipid complexes as molecular nanocapsules for conjugated linoleic acid. Biomacromolecules 6, 121–130 (2005)
J.-L. Putaux, M.B. Cardoso, D. Dupeyre, M. Morin, A. Nulac, Hu Y. Single crystals of V-amylose inclusion complexes. Macromol. Symp. 273, 1–8 (2008)
G. Wulff, G. Avgenaki, M.S.P. Guzmann, Molecular encapsulation of flavours as helical inclusion complexes of amylose. J. Cereal Sci. 41, 239–249 (2005)
S. Zabar, U. Lesmes, I. Katz, E. Shimoni, H. Bianco-Peled, Structural characterization of amylose-long chain fatty acid complexes produced via the acidification method. Food Hydrocoll. 24, 347–357 (2010)
C.G. Biliaderis, G. Galloway, Crystallization behavior of amylose-v complexes: Structure-property relationships. Carbohydr. Res. 189, 31–48 (1989)
W.C. Obiro, S. Sinha Ray, M.N. Emmambux, V-amylose structural characteristics, methods of preparation, significance, and potential applications. Food Rev. Intl. 28, 412–438 (2012)
G. Zhang, M.D. Maladen, B.R. Hamaker, Detection of a novel three component complex consisting of starch, protein, and free fatty acids. J. Agric. Food Chem. 51, 2801–2805 (2003)
P. Guerrero, J.P. Kerry, De La Caba K. FTIR characterization of protein-polysaccharide interactions in extruded blends. Carbohydr. Polym. 111, 598–605 (2014)
Y. Zhang, Q. Zhong, Effects of thermal denaturation on binding between Bixin and whey protein. J. Agric. Food Chem. 60, 7526–7531 (2012)
S.C. Sutter, P. Buera, B.E. Elizalde, Beta-carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion. Int. J. Pharm. 332, 45–54 (2007)
D.B. Rodriguez-Amaya, A Guide to carotenoide analysis in foods (ILSI PRESS, Washington, D.C, 2001)
W. Rahmalia, J.-F. Fabre, T. Usman, Z. Mouloungui, Aprotic solvents effect on the UV-visible absortion spectra of bixin. Spectrochim. Acta A Mol. Biomol. Spectrosc. 131, 455–460 (2014)
D. Tupuna, K. Paese, S. Guterres, A. Jablonski, S. Flôres, A. Rios, Encapsulation e fficiency and thermal stability of norbixin microencapsulated by spray-drying using different combinations of wall materials. Ind. Crop Prod. 111, 846–855 (2018)
U. Lesmes, J. Barchechath, E. Shimoni, Continuous dual feed homogenization for the production of starch inclusion complexes for controlled release of nutrients. Innovative Food Sci. Emerg. Technol. 9, 507–515 (2008)
J. Kim, T. Seo, S. Lim, Preparation of aqueous dispersion of b-carotene nano-composites through complex formation with starch dextrin. Food Hydrocoll. 33, 256–263 (2013)
J. Karkalas, S. Ma, W.R. Morrison, Pethrick R a. Some factors determining the thermal properties of amylose inclusion complexes with fatty acids. Carbohydr. Res. 268, 233–247 (1995)
Y. Zhang, Q. Zhong, Binding between bixin and whey protein at pH 7.4 studied by spectroscopy and isothermal titration calorimetry. J. Agric. Food Chem. 60, 1880–1886 (2012)
F.J. Monahan, J.B. German, J.E. Kinsellat, Effect of pH and temperature on protein unfolding and thiol/ disulfide interchange reactions during heat-induced gelation of whey proteins. J. Agric. Food Chem. 43, 46–52 (1995)
F.C. Felker, J.A. Kenar, G.F. Fanta, A. Biswas, Comparison of microwave processing and excess steam jet cooking for spherulite production from amylose-fatty acid inclusion complexes. Starch-Starke 65, 864–874 (2013)
Z. Czuchajowska, D. Sievert, Y. Pomeranz, Enzyme-resistant starch . IV . Effects of Complexing Lipids. Cereal Chem. 68, 537–542 (1991)
B.R. Singh, in Infrared Analysis of Peptides and Proteins: Principles and Aplication, ed. by B. R. Sing. Basic aspects of the technique and applications of infrared spectroscopy of peptides and proteins (ACS symposium serie, Washington, D.C, 2000), p. 190
Y. Zhang, Q. Zhong, Encapsulation of bixin in sodium caseinate to deliver the colorant in transparent dispersions. Food Hydrocoll. 33, 1–9 (2013)
G.A. Rocha, C.S. Fávaro-Trindade, C.R.F. Grosso, Microencapsulation of lycopene by spray drying: Characterization, stability and application of microcapsules. Food Bioprod. Process. 90, 37–42 (2012)
B. Shu, W. Yu, Y. Zhao, X. Liu, Study on microencapsulation of lycopene by spray-drying. J. Food Eng. 76, 664–669 (2006)
A. Madene, M. Jacquot, Review flavour encapsulation and controlled release – A review. Int. J. Food Sci. Technol. 41, 1–21 (2006)
R. De Marco, A.M.S. Vieira, M.C.A. Ugri, A.R.G. Monteiro, R.D.C. Bergamasco, Microencapsulation of annatto seed extract: Stability and application. Chem. Eng. Trans. 32, 1777–1782 (2013)
D.L. Boschetto, R.A. Loss, G.N. Pereira, G.S.P. Aguiar, J.R. Machado, L.M.P.C. Chaves, et al., Encapsulation of eugenyl acetate in PHBV using SEDS technique and in vitro release evaluation. J. Food Sci. Technol. 53, 3859–3864 (2014)
M.A. Montenegro, A.D.O. Rios, A.Z. Mercadante, M.A. Nazareno, C.D. Borsarelli, Model studies on the photosensitized isomerization of Bixin. J. Agric. Food Chem. 52, 367–373 (2004)
O. Rios A de, C. Borsarelli, M. Adriana, Thermal degradation kinetics of Bixin in an aqueous model system. J. Agric. Food Chem. 53, 2307–2311 (2005)
J.a. Putseys, L. Lamberts, J.a. Delcour, Amylose-inclusion complexes: Formation, identity and physico-chemical properties. J. Cereal Sci. 51, 238–247 (2010)
Acknowledgements
The authors thank the Conselho Nacional para o Desenvolvimento Científico e Tecnológico (CNPq, Grant 476927/2012-9) for their financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pérez-Monterroza, E.J., Chaux-Gutiérrez, A.M., Franco, C.M.L. et al. Encapsulation of Bixin with High Amylose Starch as Affected by Temperature and Whey Protein. Food Biophysics 13, 343–352 (2018). https://doi.org/10.1007/s11483-018-9540-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11483-018-9540-9