Skip to main content
Log in

Encapsulation of Bixin with High Amylose Starch as Affected by Temperature and Whey Protein

  • ORIGINAL ARTICLE
  • Published:
Food Biophysics Aims and scope Submit manuscript

Abstract

Microencapsulation of bixin using high-amylose corn starch was carried out by the acidification method. Bixin powders were characterized by differential scanning calorimetry (DSC), X-ray diffractometry (XRD), FT-IR spectrometry, color parameters, encapsulation efficiency, bixin release profile. In addition, the effect of whey protein (WP) on the microencapsulation process was investigated. The results obtained from DSC, X-ray diffraction and FT-IR spectrometry indicated that only in the samples prepared at 90 °C (B0WP90°C, B10WP90°C, and B20WP90°C) there was formation of crystalline structures, with melting temperatures at 117.2°, 105° and 104 °C, respectively. The possible interactions between bixin, WP and amylose starch are also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. R.E. Wrolstad, C.A. Culver, Alternatives to those Artificial FD&C Food Colorants. Annu. Rev. Food Sci. Technol. 3, 59–77 (2012)

    Article  CAS  Google Scholar 

  2. P. Giridhar, A. Venugopalan, R.A. Parimalan, Review on annatto dye extraction , analysis and processing – A food technology perspective. JSRR. 3, 327–348 (2014)

    Article  Google Scholar 

  3. C.R. Cardarelli, Benassi M de T, Mercadante AZ. Characterization of different annatto extracts based on antioxidant and colour properties. LWT Food Sci. Technol. 41, 1689–1693 (2008)

    Article  CAS  Google Scholar 

  4. M. Abayomi, A.S. Adebayo, D. Bennett, R. Porter, J. Shelly-Campbell, In vitro antioxidant activity of Bixa orellana (annatto) seed extract. JAPS. 4, 101–106 (2014)

    Google Scholar 

  5. A.D.O. Rios, L.M.G. Antunes, L.P. Bianchi M de, Proteção de carotenóides contra radicais livres gerados no tratamento de câncer com cisplatina. Alim. Nutr. 20, 343–350 (2009)

    CAS  Google Scholar 

  6. T. Taham, F.A. Cabral, M.A.S. Barrozo, Extraction of bixin from annatto seeds using combined technologies. J. Supercrit. Fluids 100, 175–183 (2015)

    Article  CAS  Google Scholar 

  7. K. Balaswamy, P.G. Prabhakara Rao, A. Satyanarayana, D.G. Rao, Stability of bixin in annatto oleoresin and dye powder during storage. LWT Food Sci. Technol. 39, 952–956 (2006)

    Article  CAS  Google Scholar 

  8. A.L. Parize, T. Cristina, S.R. De, Maria I, Brighente C, Fávere VT De, et al. microencapsulation of the natural urucum pigment with chitosan by spray drying in different solvents. Afr. J. Biotechnol. 7, 3107–3114 (2008)

    CAS  Google Scholar 

  9. M.I.M. Barbosa, C. Borsarelli, A. Mercadante, Light stability of spray-dried bixin encapsulated with different edible polysaccharide preparations. Food Res. Int. 38, 989–994 (2005)

    Article  CAS  Google Scholar 

  10. K.B. De Sousa Lobato, K. Paese, J. Casanova, S. Stanisçuaski, A. Jablonski, A. De Oliveira, Characterisation and stability evaluation of bixin nanocapsules. Food Chem. 141, 3906–3912 (2013)

    Article  Google Scholar 

  11. S.M. Lyng, M. Passos, J.D. Fontana, Bixin and α-cyclodextrin inclusion complex and stability tests. Process Biochem. 40, 865–872 (2005)

    Article  CAS  Google Scholar 

  12. V.A. Marcolino, G.M. Zanin, L.R. Durrant, M.D.T. Benassi, G. Matioli, Interaction of curcumin and bixin with β-cyclodextrin: Complexation methods, stability, and applications in food. J. Agric. Food Chem. 59, 3348–3357 (2011)

    Article  CAS  Google Scholar 

  13. T. Itthisoponkul, J.R. Mitchell, A.J. Taylor, Farhat I a. Inclusion complexes of tapioca starch with flavour compounds. Carbohydr. Polym. 69, 106–115 (2007)

    Article  CAS  Google Scholar 

  14. I. Lalush, H. Bar, I. Zakaria, S. Eichler, E. Shimoni, Utilization of amylose-lipid complexes as molecular nanocapsules for conjugated linoleic acid. Biomacromolecules 6, 121–130 (2005)

    Article  CAS  Google Scholar 

  15. J.-L. Putaux, M.B. Cardoso, D. Dupeyre, M. Morin, A. Nulac, Hu Y. Single crystals of V-amylose inclusion complexes. Macromol. Symp. 273, 1–8 (2008)

    Article  CAS  Google Scholar 

  16. G. Wulff, G. Avgenaki, M.S.P. Guzmann, Molecular encapsulation of flavours as helical inclusion complexes of amylose. J. Cereal Sci. 41, 239–249 (2005)

    Article  CAS  Google Scholar 

  17. S. Zabar, U. Lesmes, I. Katz, E. Shimoni, H. Bianco-Peled, Structural characterization of amylose-long chain fatty acid complexes produced via the acidification method. Food Hydrocoll. 24, 347–357 (2010)

    Article  CAS  Google Scholar 

  18. C.G. Biliaderis, G. Galloway, Crystallization behavior of amylose-v complexes: Structure-property relationships. Carbohydr. Res. 189, 31–48 (1989)

    Article  CAS  Google Scholar 

  19. W.C. Obiro, S. Sinha Ray, M.N. Emmambux, V-amylose structural characteristics, methods of preparation, significance, and potential applications. Food Rev. Intl. 28, 412–438 (2012)

    Article  CAS  Google Scholar 

  20. G. Zhang, M.D. Maladen, B.R. Hamaker, Detection of a novel three component complex consisting of starch, protein, and free fatty acids. J. Agric. Food Chem. 51, 2801–2805 (2003)

    Article  CAS  Google Scholar 

  21. P. Guerrero, J.P. Kerry, De La Caba K. FTIR characterization of protein-polysaccharide interactions in extruded blends. Carbohydr. Polym. 111, 598–605 (2014)

    Article  CAS  Google Scholar 

  22. Y. Zhang, Q. Zhong, Effects of thermal denaturation on binding between Bixin and whey protein. J. Agric. Food Chem. 60, 7526–7531 (2012)

    Article  CAS  Google Scholar 

  23. S.C. Sutter, P. Buera, B.E. Elizalde, Beta-carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion. Int. J. Pharm. 332, 45–54 (2007)

    Article  CAS  Google Scholar 

  24. D.B. Rodriguez-Amaya, A Guide to carotenoide analysis in foods (ILSI PRESS, Washington, D.C, 2001)

    Google Scholar 

  25. W. Rahmalia, J.-F. Fabre, T. Usman, Z. Mouloungui, Aprotic solvents effect on the UV-visible absortion spectra of bixin. Spectrochim. Acta A Mol. Biomol. Spectrosc. 131, 455–460 (2014)

    Article  CAS  Google Scholar 

  26. D. Tupuna, K. Paese, S. Guterres, A. Jablonski, S. Flôres, A. Rios, Encapsulation e fficiency and thermal stability of norbixin microencapsulated by spray-drying using different combinations of wall materials. Ind. Crop Prod. 111, 846–855 (2018)

    Article  CAS  Google Scholar 

  27. U. Lesmes, J. Barchechath, E. Shimoni, Continuous dual feed homogenization for the production of starch inclusion complexes for controlled release of nutrients. Innovative Food Sci. Emerg. Technol. 9, 507–515 (2008)

    Article  CAS  Google Scholar 

  28. J. Kim, T. Seo, S. Lim, Preparation of aqueous dispersion of b-carotene nano-composites through complex formation with starch dextrin. Food Hydrocoll. 33, 256–263 (2013)

    Article  CAS  Google Scholar 

  29. J. Karkalas, S. Ma, W.R. Morrison, Pethrick R a. Some factors determining the thermal properties of amylose inclusion complexes with fatty acids. Carbohydr. Res. 268, 233–247 (1995)

    Article  CAS  Google Scholar 

  30. Y. Zhang, Q. Zhong, Binding between bixin and whey protein at pH 7.4 studied by spectroscopy and isothermal titration calorimetry. J. Agric. Food Chem. 60, 1880–1886 (2012)

    Article  CAS  Google Scholar 

  31. F.J. Monahan, J.B. German, J.E. Kinsellat, Effect of pH and temperature on protein unfolding and thiol/ disulfide interchange reactions during heat-induced gelation of whey proteins. J. Agric. Food Chem. 43, 46–52 (1995)

    Article  CAS  Google Scholar 

  32. F.C. Felker, J.A. Kenar, G.F. Fanta, A. Biswas, Comparison of microwave processing and excess steam jet cooking for spherulite production from amylose-fatty acid inclusion complexes. Starch-Starke 65, 864–874 (2013)

    Article  CAS  Google Scholar 

  33. Z. Czuchajowska, D. Sievert, Y. Pomeranz, Enzyme-resistant starch . IV . Effects of Complexing Lipids. Cereal Chem. 68, 537–542 (1991)

    CAS  Google Scholar 

  34. B.R. Singh, in Infrared Analysis of Peptides and Proteins: Principles and Aplication, ed. by B. R. Sing. Basic aspects of the technique and applications of infrared spectroscopy of peptides and proteins (ACS symposium serie, Washington, D.C, 2000), p. 190

    Google Scholar 

  35. Y. Zhang, Q. Zhong, Encapsulation of bixin in sodium caseinate to deliver the colorant in transparent dispersions. Food Hydrocoll. 33, 1–9 (2013)

    Article  Google Scholar 

  36. G.A. Rocha, C.S. Fávaro-Trindade, C.R.F. Grosso, Microencapsulation of lycopene by spray drying: Characterization, stability and application of microcapsules. Food Bioprod. Process. 90, 37–42 (2012)

    Article  Google Scholar 

  37. B. Shu, W. Yu, Y. Zhao, X. Liu, Study on microencapsulation of lycopene by spray-drying. J. Food Eng. 76, 664–669 (2006)

    Article  CAS  Google Scholar 

  38. A. Madene, M. Jacquot, Review flavour encapsulation and controlled release – A review. Int. J. Food Sci. Technol. 41, 1–21 (2006)

    Article  CAS  Google Scholar 

  39. R. De Marco, A.M.S. Vieira, M.C.A. Ugri, A.R.G. Monteiro, R.D.C. Bergamasco, Microencapsulation of annatto seed extract: Stability and application. Chem. Eng. Trans. 32, 1777–1782 (2013)

    Google Scholar 

  40. D.L. Boschetto, R.A. Loss, G.N. Pereira, G.S.P. Aguiar, J.R. Machado, L.M.P.C. Chaves, et al., Encapsulation of eugenyl acetate in PHBV using SEDS technique and in vitro release evaluation. J. Food Sci. Technol. 53, 3859–3864 (2014)

    Google Scholar 

  41. M.A. Montenegro, A.D.O. Rios, A.Z. Mercadante, M.A. Nazareno, C.D. Borsarelli, Model studies on the photosensitized isomerization of Bixin. J. Agric. Food Chem. 52, 367–373 (2004)

    Article  CAS  Google Scholar 

  42. O. Rios A de, C. Borsarelli, M. Adriana, Thermal degradation kinetics of Bixin in an aqueous model system. J. Agric. Food Chem. 53, 2307–2311 (2005)

    Article  Google Scholar 

  43. J.a. Putseys, L. Lamberts, J.a. Delcour, Amylose-inclusion complexes: Formation, identity and physico-chemical properties. J. Cereal Sci. 51, 238–247 (2010)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank the Conselho Nacional para o Desenvolvimento Científico e Tecnológico (CNPq, Grant 476927/2012-9) for their financial support.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ezequiel José Pérez-Monterroza.

Electronic supplementary material

Fig. 6

(Supplementary Figure). UV-vis absorbance of bixin and samples at 60 °C (a), 70 °C (b) and 90 °C (DOCX 192 kb)

ESM 1

(DOCX 14 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pérez-Monterroza, E.J., Chaux-Gutiérrez, A.M., Franco, C.M.L. et al. Encapsulation of Bixin with High Amylose Starch as Affected by Temperature and Whey Protein. Food Biophysics 13, 343–352 (2018). https://doi.org/10.1007/s11483-018-9540-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11483-018-9540-9

Keywords

Navigation