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Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers

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Abstract

Up to 73% decrease in cost of media for plant regeneration and in vitro conservation was achieved in Curcuma longa cv Prathibha by using inexpensive carbon source and gelling agent. Laboratory reagent-grade sucrose was replaced by locally available commercial sugar (market sugar or sugar cubes) as carbon source and bacteriological grade agar by isabgol (also named isubgol) as gelling agent. No adverse effects on shoot regeneration and conservation on isabgol-gelled low-cost media were observed as compared to that on agar-gelled control medium (CM). Some 33–56% cultures of C. longa survived up to 12 mo. on isabgol-gelled medium in comparison to only 16% on CM. Genetic stability of 12-month-old in vitro-conserved plants was assessed using 25 random amplified polymorphic DNA (RAPD) primers; no significant variation was observed in RAPD profiles of mother plants and in vitro-conserved plantlets on CM and low-cost media.

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References

  • Ashmore, S.E. Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. Rome: International Plant Genetic Resources Institute; 1997:1–67.

    Google Scholar 

  • Babbar, S.B.; Jain, N. ‘Isubgol’ as an alternative gelling agent in plant tissue culture media. Plant Cell Rep. 17:318–322; 1998.

    Article  CAS  Google Scholar 

  • Balachandran, S.M.; Bhat, S.R.; Chandel, K.P.S. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8:521–524; 1990.

    Article  CAS  Google Scholar 

  • Bhattacharya, P.; Dey, S.; Bhattacharyya, B.C. Use of low-cost gelling agents and support matrices for industrial scale plant tissue culture. Plant Cell Tissue Organ Cult. 37:15–23; 1994.

    Article  Google Scholar 

  • Brunk, C.F.; Jones, K.C.; James, T.W. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem. 92:497–500; 1979.

    Article  PubMed  CAS  Google Scholar 

  • Cameron, S.I. Tissue culture gel firmness: measurement and effects on growth. In: Gupta, S.D.; Ibaraki, Y., eds. Plant tissue culture engineering. Netherlands: Springer; 2006:329–337.

    Chapter  Google Scholar 

  • Debergh, P.C. Effects of agar brand and concentration on the tissue culture medium. Physiol Plant. 59:270–276; 1983.

    Article  CAS  Google Scholar 

  • Eberhard, S.; Doubrava, N.; Marfà, V.; Mohnen, D.; Southwick, A.; Darvill, A.; Albersheim, P. Pectic cell wall fragments regulate tobacco thin-call-layer explant morphogenesis. Plant Cell 1:747–755; 1989.

    Article  PubMed  CAS  Google Scholar 

  • Fischer, M.H.; Yu, N.; Gary, G.R.; Ralph, J.; Anderson, L.; Marlett, J.A. The gel-forming polysaccharide of psyllium husk (Plantago ovata Forsk). Carbohydr Res. 339:2009–2017; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Ganapathi, T.R.; Mohan, J.S.S.; Suprasanna, P.; Bapat, V.A.; Rao, P.S. A low-cost strategy for in vitro propagation of banana. Curr Sci. 68:646–650; 1995.

    Google Scholar 

  • IAEA. Low cost options for tissue culture technology in developing countries. Vienna: International Atomic Energy Agency, IAEA-TECDOC-1384; 2004: 1–102.

    Google Scholar 

  • Jain, R.; Babbar, S.B. Guar gum and isubgol as cost-effective alternative gelling agents for in vitro multiplication of an orchid, Dendrobium chrysotoxum. Curr Sci. 88:292–295; 2005.

    Google Scholar 

  • Janardhan, K.V.; Murthy, A.S.P.; Giriraj, K.; Ksharaias, S.P. A rapid method for determination of osmotic potential of plant cell sap. Curr Sci. 44:390–391; 1975.

    Google Scholar 

  • Jarret, R.L.; Florkowski, W.J. In vitro active vs. field genebank maintenance of sweet potato germplasm: major costs and other considerations. HortScience 25:141–146; 1990.

    Google Scholar 

  • Kimball, S.L.; Beversdorf, W.D.; Bingham, E.T. Influence of osmotic potential on the growth and development of soybean tissue cultures. Crop Sci. 15:750–752; 1975.

    Article  CAS  Google Scholar 

  • Koo, B.; Pardey, P.; Wright, B. The price of conserving agricultural biodiversity. Nat Biotechnol. 21:126–128; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Letarte, J.; Simion, E.; Miner, M.; Kasha, K.J. Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep. 24:691–698; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Mandal, B.B.; Tyagi, R.K.; Pandey, R.; Sharma, N.; Agrawal, A. In vitro conservation of germplasm of agri-horticultural crops at NBPGR: an overview. In: Razdan, M.K.; Cocking E.C., eds. Conservation of plant genetic resources in vitro, vol. 2: application and limitations. USA: Science Publishers, Inc.; 2000:297–307.

    Google Scholar 

  • Martins, M.; Sarmento, D.; Oliveira, M.M. Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers. Plant Cell Rep. 23:492–496; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Owens, L.D.; Wozniak, C.A. Measurement and effects of gel matric potential and expressibility on production of morphogenic callus by cultured sugarbeet leaf discs. Plant Cell Tissue Organ Cult. 26:127–133; 1991.

    Article  Google Scholar 

  • Purseglove, J.W.; Brown, E.G.; Green, C.L.; Robbins, S.R. Spices, vol. 2. London and New York: Longman; 1981:440–813.

    Google Scholar 

  • Ravindran, P.N.; Nirmal Babu, K.; Peter, K.V.; Abraham, Z.; Tyagi, R.K. Spices. In: Dhillon, B.S.; Tyagi, R.K.; Saxena, S.; Randhawa, G.J., eds. Plant genetic resources: horticultural plants. New Delhi: Narosa Publishing House; 2005:190–227.

    Google Scholar 

  • Saghai-Maroof, M.A.; Soliman, K.M.; Jorgenson, R.A.; Allard, A.W. Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci U S A 81:8014–8018; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Salvi, N.D.; George, L.; Eapen, S. Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tissue Organ Cult. 66:113–119; 2001.

    Article  CAS  Google Scholar 

  • Tyagi, R.K.; Yusuf, A.; Dua, P.; Agrawal, A. In vitro plant regeneration and genotype conservation of eight wild species of Curcuma. Biol. Plant. 48:129–132; 2004.

    Article  CAS  Google Scholar 

  • WOI. Saccharum. In: The wealth of India: a dictionary of Indian raw materials & industrial products—raw materials, vol IX: Rh-So. New Delhi: Publications and Information Directorate, Council for Scientific and Industrial Research; 1981:96–165.

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Acknowledgments

The authors are grateful to the director, NBPGR, New Delhi, for providing the facilities. Special thanks are accorded to Dr. Ana M. Vieitez, the associate editor of this journal, whose critique has greatly helped in the improvement of the manuscript.

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Authors and Affiliations

  1. Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, 110012, India

    Rishi K. Tyagi, Anuradha Agrawal, C. Mahalakshmi, Zakir Hussain & Husnara Tyagi

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  1. Rishi K. Tyagi
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  2. Anuradha Agrawal
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  3. C. Mahalakshmi
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  4. Zakir Hussain
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  5. Husnara Tyagi
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Correspondence to Rishi K. Tyagi.

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Editor: A. Vieitez

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Tyagi, R.K., Agrawal, A., Mahalakshmi, C. et al. Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers. In Vitro Cell.Dev.Biol.-Plant 43, 51–58 (2007). https://doi.org/10.1007/s11627-006-9000-y

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  • DOI: https://doi.org/10.1007/s11627-006-9000-y

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