Abstract
Oligo-carrageenans (OCs) obtained from pure carrageenans extracted from marine red algae stimulate growth by enhancing photosynthesis and basal metabolism in tobacco plants and Eucalyptus trees. In addition, OCs stimulate secondary metabolism, increasing the level of metabolites involved in defense against pathogens. In this work, we analyzed the effect of OC kappa on the increase in height, in activities of basal metabolism enzymes involved in carbon, nitrogen and sulphur assimilation, ribulose 1,5 biphosphate carboxylase/oxygenase (rubisco), glutamate dehydrogenase (GDH) and O-acetylserine thiol-lyase (OASTL), and in the level of growth-promoting hormones, the auxin indole acetic acid (IAA) and the gibberellin GA3, in pine (Pinus radiata) trees treated with OC kappa at concentrations of 1 and 5 mg mL−1 and cultivated for 9 months without additional treatment. Pines treated with OC kappa at 1 mg mL−1 showed a similar increase in height but displayed a higher increased in total chlorophyll, activities of rubisco, GDH and OASTL and level of IAA and GA3 than those treated with OC kappa at 5 mg mL−1. Thus, OC kappa stimulates growth and basal metabolism and increases the level of growth-promoting hormones in pine trees, mainly at 1 mg mL−1.
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Albersheim P, Darvill A, Augur C, Cheong JJ, Eberherd S, Hahn MG, Marfá V, Mohnen D, O’Neill MA, Spiro MD, York WS (1992) Oligosaccharins-oligosaccharide regulatory molecules. Acc Chem Res 25:7783
Björklund S, Antti H, Uddestrand I, Moritz T, Sundberg B (2007) Cross talk between gibberellin and auxin in development of Populus wood: gibberellins stimulate polar auxin transport and has a common transcriptome with auxin. Plant J 52:499511
Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248254
Castro J, Vera J, González A, Moenne A (2012) Oligo-carrageenans stimulate growth by enhancing photosynthesis, basal metabolism, and cell cycle in tobacco plants (var. Burley). J Plant Growth Regul 31:173185
Clarke JT, Warnock RC, Donoghe PCJ (2011) Establishing a time-scale for plant evolution. New Phytol 192:266301
Falkenberg B, Witt I, Zanór MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R (2008) Transcription factors relevent to auxin signaling coordinate broad-spectrum metabolic shifts including sulphur metabolism. J Exp Bot 59:28312846
Frigeiro L, Alabadi D, Pérez-Gómez J, Gracia-Cacel L, Phillips AL, Hedden P, Blazquez MA (2006) Transcriptional regulation of gibberellin metabolism genes by auxin signaling in Arabidopsis. Plant Physiol 142:553563
González A, Castro J, Vera J, Moenne A (2013a) Seaweed oligosaccharides stimulate plant growth by enhancing carbon and nitrogen assimilation, basal metabolism and cell division. J Plant Growth Regul 32:443448
González A, Contreras RA, Moenne A (2013b) Oligo-carrageenans enhance growth and contents of cellulose, essential oils and polyphenolic compounds in Eucalyptus globulus trees. Molecules 18:87408751
González A, Contreras RA, Zuñiga G, Moenne A (2014a) Oligo-carrageenan kappa-induced reducing redox status and activation of TRR/TRX system increase the level of indole-3-acetic acid, gibberellin A3 and trans-zeatin in Eucalyptus globulus trees. Molecules 19:1269012698
González A, Gutierrez-Cutiño M, Moenne A (2014b) Oligo-carrageenan kappa-induced reducing redox status and increase in TRR/TRX activities promote activation and reprogramming of terpenoid metabolism in Eucalyptus trees. Molecules 19:7356–7367
González A, Moenne F, Gómez M, Sáez CA, Contreras RA, Moenne A (2014c) Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees. Front Plant Sci 5:512
Kopriva S, Suter M, Von Ballmoos O, Hesse H, Krähenbühl U, Rennenberg H, Brunold C (2002) Interaction of sulfate metabolism with carbon/nitrogen metabolism in Lemna minor. Plant Physiol 130:14061413
Kraiser T, De Gras AG, Gutiérrez AG, González B, Gutiérrez RA (2011) Holistic view of nitrogen acquisition in plants. J Exp Bot 62:14551466
Li ZY, Xu ZS, Chen Y, He GY, Yang GY, Chen M, Li LC, Ma YZ (2013) A novel role for Arabidopsis CBL1 in affecting plant response to glucose and gibberellins during germination and seedling development. PLoS One 8:e56412
Lichtenthaler H, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b in leaf extracts in different solvents. Biochem Soc Trans 11:591–592 (Your reference is not cited in the paper.)
Lilley RM, Walker DA (1974) An improved spectrophotometric assay for ribulosebiphosphate carboxylase. Biochim Biophys Acta 358:226229
Lunn J, Droux M, Martin J, Douce R (1990) Localization of ATP sulfurylase and O-acetylserine (thiol) lyase in spinach leaves. Plant Physiol 94:13451352
O’Neill DP, Ross JJ (2002) Auxin regulation of the gibberellin pathway in Arabidopsis. Plant Physiol 130:9741978
Pan X, Welti R, Wang X (2010) Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry. Nat Protoc 5:986992
Turano FJ, Dashner R, Upadhyaya A, Caldwell CR (1996) Purification of mitochondrial glutamate dehydrogenase from dark-grown soybean seedlings. Plant Physiol 112:13571364
Vera J, Castro J, González A, Moenne A (2011) Seaweed polysaccharides and derived oligosaccharides stimulate defense responses and protection against pathogens in plants. Mar Drugs 9:25142525
Vera J, Castro J, Contreras RA, González A, Moenne A (2012) Oligo-carrageenans induced a long-term and broad-range protection against pathogens and the reversion of infections in tobacco plants (var. Xanthi). Physiol Mol Plant Pathol 79:3139
Weiss D, Ori N (2007) Mechanisms of cross talk between gibberellins and other hormones. Plant Physiol 144:12401246
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This work was financed by Sirius Natura S.A. and VRIDEI-USACH. Silvia Saucedo was financed by SENESCYT-Ecuador, Convocatoria 2011.
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Saucedo, S., Contreras, R.A. & Moenne, A. Oligo-carrageenan kappa increases C, N and S assimilation, auxin and gibberellin contents, and growth in Pinus radiata trees. J. For. Res. 26, 635–640 (2015). https://doi.org/10.1007/s11676-015-0061-9
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DOI: https://doi.org/10.1007/s11676-015-0061-9