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Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis

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Abstract

Advances in plant tissue culture methods with regard to lighting requirements are currently focused on the improved features of light-emitting diodes (LEDs). Over the years, the steady development of LED technology, with the emergence of new types of semi-conductor materials, has made it possible to apply it in an increasing number of new areas. As an alternative to conventional lighting systems, LED has been demonstrated to be an artificial flexible lighting source for plant tissue culture. Numerous studies have been conducted in order to investigate the effects of LED on plants, which have led to many satisfactory results. Various morphological, anatomical, and physiological attributes, such as shoot elongation, axillary shoot formation, somatic embryo induction, rhizogenesis, leaf anatomy, and photosynthetic abilities of plants grown in vitro have found to be regulated by spectral properties of LEDs. The present review gives an overview of the fundamentals of LEDs and describes their effects on in vitro plant growth and morphogenesis and their future potentials.

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References

  • Bourget MC (2008) An introduction to light emitting diodes. HortScience 43:1944–1946

    Google Scholar 

  • Briggs WR, Olney MA (2001) Photoreceptors in plant photomorphogenesis to date, five photochromes, two cryptochrome, one phototropin and one superchrome. Plant Physiol 125:85–88

    Article  PubMed  CAS  Google Scholar 

  • Brown CS, Schuerger AC, Sager JC (1995) Growth and photomorphogenesis of pepper under red light-emitting diodes with supplemental blue or far-red lighting. J Am Soc Hortic Sci 120:808–813

    PubMed  CAS  Google Scholar 

  • Budiarto K (2010) Spectral quality affects morphogenesis on Anthurium plantlet during in vitro culture. Agrivita 32:234–240

    Google Scholar 

  • Bula RJ, Morrow RC, Tibbitts TW, Barta DJ, Ingnatius RW, Martin TS (1991) Light-emitting diodes as a radiation source for plants. HortScience 26:203–205

    PubMed  CAS  Google Scholar 

  • Chen J, Henny RJ, McConnell DB (2002) Development of new foliage plant cultivars. Florida Agric Exp Sta J R-08541:117

    Google Scholar 

  • Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2006) Flowering of Euphorbia millii plantlets in vitro as affected by paclobutrazol, light emitting diodes (LEDs). Acta Hortic 764:169–173

    Google Scholar 

  • Duong TN, Hong LTA, Watanabe H, Goi M, Tanaka M (2003) Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hortic 616:121–127

    Google Scholar 

  • Economou AS, Read PE (1987) Light treatments to improve efficiency of in vitro propagation systems. Hortic Sci 22:751–754

    Google Scholar 

  • Folta KM, Koss LL, McMorrow R, Kim HH, Kenitz JD, Wheeler R, Sager JC (2005) Design and fabrication of adjustable red-green-blue LED light arrays for plant research. BMC Plant Biol 23:5–17

    Google Scholar 

  • Godo T, Fujiwara K, Guan K, Miyoshi K (2011) Effect of wavelength of LED-light on in vitro asymbiotic germination and seedling growth of Bletilla ochracea schltr (Orchidaceae). Plant Biotechnol 28:397–400

    Article  Google Scholar 

  • Goins GD, Yorio NC, Sanwo MM, Brown CS (1997) Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LED) with and without supplemental blue lighting. J Exp Bot 48:1407–1413

    Article  PubMed  CAS  Google Scholar 

  • Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets. J Plant Biol 43:247–250

    Article  Google Scholar 

  • Heo JW, Lee CW, Chakrabarty D, Paek KY (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light emitting diode (LED). J Plant Growth Regul 38:225–230

    Article  CAS  Google Scholar 

  • Heo JW, Shin KS, Kim SK, Paek KY (2006) Light quality affects in vitro growth of grape ‘Teleki 5BB7’. J Plant Biol 49:276–280

    Article  Google Scholar 

  • Hoenecke ME, Bula RJ, Tibbitts TW (1992) Importance of ‘blue’ photon levels for lettuce seedlings grown under red-light-emitting diodes. HortScience 27:427–430

    PubMed  CAS  Google Scholar 

  • Huan LVT, Tanaka M (2004) Effects of red and blue light-emitting diodes on callus induction, callus proliferation and protocorm like body formation from callus in Cymbidium orchid. Environ Contr Biol 42(1):57–64

    Article  Google Scholar 

  • Jao RC, Fang W (2004a) Effects of frequency and duty ratio on the growth of potato plantlets in vitro using light emitting diode. HortScience 39:375–379

    Google Scholar 

  • Jao RC, Fang W (2004b) Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods. HortScience 39:380–382

    Google Scholar 

  • Jao RC, Lai CC, Fang W, Chang SF (2005) Effect of red light on the growth of Zantedeschia plantlets in vitro and tuber formation using light emitting diodes. HortScience 40(2):436–438

    Google Scholar 

  • Kim HH, Goins GD, Wheeler RM, Sager JC (2004a) Green light supplementation for enhanced lettuce growth under red and blue light emitting diodes. HortScience 39:1617–1622

    PubMed  Google Scholar 

  • Kim HH, Goins GD, Wheeler RM, Sager JC (2004b) Stomatal conductance of lettuce grown under or exposed to different light qualities. Ann Bot 94:691–697

    Article  PubMed  Google Scholar 

  • Kim SJ, Hahn EJ, Heo JW, PaeK KY (2004c) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of Chrysanthemum plantlets in vitro. Sci Hortic 101:143–151

    Article  Google Scholar 

  • Kowallik W (1982) Blue light effects on respiration. Plant Physiol 33:51–72

    Article  CAS  Google Scholar 

  • Krapiel Y, Mipiniac E (1997) Photomorphogenesis and phytohormones. Plant Cell Environ 20:807–812

    Article  Google Scholar 

  • Kurilcik A, Canova MR, Dapkuniene S, Zilinskaite S, Kurilcik G, Tamulaitis G, Duchovskis P, Zukauskas A (2008) In vitro culture of Chrysanthemum plantlets using light emitting diodes. Cent Eur J Biol 3:161–167

    Article  Google Scholar 

  • Li H, Xu Z, Tang C (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell Tissue Organ Cult 103:155–163

    Article  Google Scholar 

  • Lian ML, Murthy HN, Paek KY (2002) Effects of light emitting diodes (LED) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Sci Hortic 94:365–370

    Article  Google Scholar 

  • Lin Y, Li J, Li B, He T, Chun Z (2010) Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell Tissue Organ Cult 105:329–335

    Article  Google Scholar 

  • Massa GD, Kim HH, Wheeler RM, Mitchell CA (2008) Plant productivity in response to LED lighting. HortScience 43:1951–1956

    Google Scholar 

  • Mengxi L, Zhigang X, Yang Y, Yijie F (2010) Effects of different spectral lights on Oncidium PLBs induction, proliferation and plant regeneration. Plant Cell Tissue Organ Cult 106(1):1–10

    Article  Google Scholar 

  • Merkle SA, Montello PM, Xia X, Upchurch BL, Smith DR (2005) Light quality treatments enhance somatic seedling production in three southern pine species. Tree Physiol 26:187–194

    Article  Google Scholar 

  • Moon HK, Park SK, Kim YW, Kim CS (2006) Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. J Plant Biol 49:174–179

    Article  Google Scholar 

  • Morrow RC (2008) LED lighting in horticulture. HortScience 43:1947–1950

    Google Scholar 

  • Nakamura S, Fasol G, Pearton SJ (2000) The blue laser diode: the complete story. Springer, Dordrecht

    Book  Google Scholar 

  • Nhut DT, Nam NB (2010) Light emitting diodes (LEDs): an artificial lighting source for biological studies. In: Proceedings of the 3rd international conference of the development of BME in Vietnam, 11–14 January 2010

  • Nhut DT, Hong LTA, Watanabe H, Goi M, Tanaka M (2000) Growth of banana plantlets cultured in vitro under red and blue light-emitting diode (LED) irradiation source. Acta Hortic 575:7–23

    Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Murakami A, Murakami K, Tanaka M (2002) Sugar-free micropropagation of Eucalyptus citriodora using light-emitting diode (LEDs) and film-rockwool culture system. Environ Control Biol 40:147–155

    Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under super-bright red and blue light-emitting diodes (LED). Plant Cell Tissue Organ Cult 73:43–52

    Article  CAS  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2005) Artificial light source using light-emitting diodes (LEDs) in the efficient micropropagation of Spathiphyllum plantlets. Acta Hortic 692:137–142

    Google Scholar 

  • Nhut TD, Loan PTL, Thao PTL, Suong TTN, Lien HV (2006) Effects of some artificial lighting sources on the photomorphogenesis of callus induced embryo and the plantlet regeneration of Phalaenopsis amabilis and its applications in micropropagation. In: Proceedings of International Workshop on Agricultural Biotechnology, pp 20–21

  • Ogren E (2006) Connecting LEDs: serial vs. parallel. http://www.analogictech.com/uploads/technicalarticles/TA-101:1-3. Accessed 12 May 2012

  • Park SY, Edward YC, Paek KY (2010) Endoreduplication in Phalaenopsis is affected by light quality from light-emitting diodes during somatic embryogenesis. Plant Biotechnol Rep 4:303–309

    Article  CAS  Google Scholar 

  • Poudel RP, Kataoka I, Mochioka R (2008) Effect of red-and blue-lightemitting diodes on growth and morphogenesis of grapes. Plant Cell Tissue Organ Cult 92:147–153

    Article  Google Scholar 

  • Puspa RP, Kataoka I, Mochioka R (2008) Effect of red-and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Organ Cult 92:147–153

    Google Scholar 

  • Richardson C (2008) Driving high-power LEDs in series-parallel arrays. National Semiconductor, November 27, pp 45–49. http://www.globaltechled.com/Resources/LED%20driving%20series-parallel. Accessed 25 May 2012

  • Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsium annuum L.) grown under red light emitting diodes supplemented with blue or far-red light. Ann Bot 79:273–282

    Article  PubMed  CAS  Google Scholar 

  • Seabrook JEA (2005) Light effects on the growth and morphogenesis of potato (Solanum tuberosum) in vitro: a review. Am J Pot Res 82(5):353–367

    Article  Google Scholar 

  • Shin SK, Murthy NH, Heo WJ, Hahn JE, Paek YK (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plantae 30:339–343

    Article  CAS  Google Scholar 

  • Soebo A, Krekling T, Appelgren M (1995) Light quality effects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tissue Organ Cult 41:177–185

    Article  Google Scholar 

  • Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth of Cymbidium plantlets cultured under super bright and blue light emitting diodes (LEDs). J Hortic Sci Biotechnol 73:39–44

    Google Scholar 

  • Tennessen DJ, Singsaasl EL, Sharkeyl TD (1994) Light-emitting diodes as a light source for photosynthesis research. Photosynth Res 39:85–92

    Article  CAS  Google Scholar 

  • Wongnok A, Piluek C, Techasilpitak T, Tantivivat S (2008) Effect of light emitting diodes on micropropagation of Phalaenopsis orchids. Acta Hortic 788:149–156

    Google Scholar 

  • Wu MC, Hou CY, Jiang CM, Wang YT, Wang CY, Chen HH, Chan HM (2007) A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. Food Chem 101:1753–1758

    Article  CAS  Google Scholar 

  • Yeh H, Chung JP (2009) High-brightness LEDs-energy efficient lighting sources and their potential in indoor plant cultivation. Renew Sust Energy Rev 13:2175–2180

    Article  CAS  Google Scholar 

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  1. Department of Agricultural and Food Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

    S. Dutta Gupta & B. Jatothu

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  1. S. Dutta Gupta
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  2. B. Jatothu
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Correspondence to S. Dutta Gupta.

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Dutta Gupta, S., Jatothu, B. Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechnol Rep 7, 211–220 (2013). https://doi.org/10.1007/s11816-013-0277-0

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  • DOI: https://doi.org/10.1007/s11816-013-0277-0

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