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Separate and Concentrate Lactic Acid Using Combination of Nanofiltration and Reverse Osmosis Membranes

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Abstract

The processes of lactic acid production include two key stages, which are (a) fermentation and (b) product recovery. In this study, free cell of Bifidobacterium longum was used to produce lactic acid from cheese whey. The produced lactic acid was then separated and purified from the fermentation broth using combination of nanofiltration and reverse osmosis membranes. Nanofiltration membrane with a molecular weight cutoff of 100–400 Da was used to separate lactic acid from lactose and cells in the cheese whey fermentation broth in the first step. The obtained permeate from the above nanofiltration is mainly composed of lactic acid and water, which was then concentrated with a reverse osmosis membrane in the second step. Among the tested nanofiltration membranes, HL membrane from GE Osmonics has the highest lactose retention (97 ± 1%). In the reverse osmosis process, the ADF membrane could retain 100% of lactic acid to obtain permeate with water only. The effect of membrane and pressure on permeate flux and retention of lactose/lactic acid was also reported in this paper.

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Authors and Affiliations

  1. Department of Food, Agricultural, and Biological Engineering, The Ohio State University, 1680 Madison Ave, Wooster, OH, 44691-4096, USA

    Yebo Li & Caixia Wan

  2. Department of Natural Resources and Environmental Design, North Carolina A&T State University, 1601 East Market Street, Greensboro, NC, 27411, USA

    Abolghasem Shahbazi & Karen Williams

Authors
  1. Yebo Li
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  2. Abolghasem Shahbazi
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  3. Karen Williams
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  4. Caixia Wan
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Correspondence to Yebo Li.

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Li, Y., Shahbazi, A., Williams, K. et al. Separate and Concentrate Lactic Acid Using Combination of Nanofiltration and Reverse Osmosis Membranes. Appl Biochem Biotechnol 147, 1–9 (2008). https://doi.org/10.1007/s12010-007-8047-5

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  • DOI: https://doi.org/10.1007/s12010-007-8047-5

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