Skip to main content
SpringerLink
Log in

Production, Characterization, and Properties of Sophorolipids from the Yeast Candida bombicola using a Low-cost Fermentative Medium

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The yeast Candida bombicola produces biosurfactant with properties akin to those of sophorolipid (SL) group of compounds. In the present work, the yeast was shown to produce 63.7 g l−1 SL when grown on a cheap fermentative medium containing sugarcane molasses, yeast extract, urea, and soybean oil. The partially purified SL was characterized and confirmed by Fourier-transform infrared (FT-IR) spectroscopy, 1H and 13C nuclear magnetic resonance (NMR) and liquid chromatography–mass spectroscopy (LC-MS) analysis. The critical micelle concentration (CMC) and minimum surface tension of the produced SL in aqueous solution were found to be 59.43 mg l−1 and 34.15 m Nm−1, respectively. The emulsification activity and stability with kerosene oil and organic solvents viz. xylene, benzene, and hexadecane were also tested using the produced SL, which yielded better results compared to those reported in the literature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Esders, T. W., & Light, R. J. (1972). The Journal of Biological Chemistry, 247, 1375–1386.

    CAS  Google Scholar 

  2. Hommel, R. K., & Huse, K. (1993). Biotechnology Letters, 15, 853–858. doi:10.1007/BF00180154.

    Article  CAS  Google Scholar 

  3. Asmer, H. J., Lang, S., Wagner, F., & Wray, V. (1988). Journal of the American Oil Chemists’ Society, 65, 1460–1466. doi:10.1007/BF02898308.

    Article  CAS  Google Scholar 

  4. Chen, J., Song, X., Zhang, H., Qu, Y. B., & Miao, J. Y. (2006). Enzyme and Microbial Technology, 39, 501–506. doi:10.1016/j.enzmictec.2005.12.022.

    Article  CAS  Google Scholar 

  5. Gorin, A. P. J., Spencer, J. F. T., & Tulloch, A. P. (1961). Canadian Journal of Chemistry, 39, 846–895. doi:10.1139/v61-104.

    Article  CAS  Google Scholar 

  6. Gross, R. A., Doncel, G., & Shah, V. (2004). US Patent. US 10/804778.

  7. Kandil, E., Zhang, H., Schulze, R., Dresner, L., Nowakowski, M., & Gross, R. A. (2003). Journal of the American College of Surgeons, 197, S40–S41.

    Google Scholar 

  8. Scholz, C., Mehta, S., Bisht, K., Guilmanov, V., Kaplan, D., & Nicolosi, R. (1998). Polymer Preprints, 39, 168–169.

    CAS  Google Scholar 

  9. Maingault, M. (1997). Canada Patent. No. CAN, 126, 242874.

    Google Scholar 

  10. Singh, S. K., Felse, A. P., Nunez, A., Foglia, T. A., & Gross, R. A. (2003). The Journal of Organic Chemistry, 68, 5466–5477. doi:10.1021/jo0204395.

    Article  CAS  Google Scholar 

  11. Hall, P. J., Haverkamp, J., van Kralingen, C. G., & Schmidt, M. (1996). US Patent. US 5,520, 839.

  12. Mulligan, C. N., Yong, R. N., & Gibbs, B. F. (2001). Journal of Hazardous Materials, 85, 111–125. doi:10.1016/S0304-3894(01)00224-2.

    Article  CAS  Google Scholar 

  13. Schippers, C., Gessner, K., Muller, T., & Scheper, T. (2000). Journal of Biotechnology, 83, 189–198. doi:10.1016/S0168-1656(00)00304-7.

    Article  CAS  Google Scholar 

  14. Bluth, M. H., Fu, S. L., Fu, A., Stanek, A., Smith-Norowitz, T. A., Wallner, S. R., et al. (2008). The Journal of Allergy and Clinical Immunology, 121, S2. doi:10.1016/j.jaci.2007.12.011.

    Google Scholar 

  15. Fu, S. L., Garnett, I., Wallner, S. R., Zenilman, M. E., Gross, R., Bluth, M. H., & QS305 (2008). Journal of Surgical Research, 144, 388. doi:10.1016/j.jss.2007.12.557.

    Article  Google Scholar 

  16. Lynd, L. R., Wyman, C. E., & Gerngross, T. U. (1999). Biotechnology Progress, 15, 777–793. doi:10.1021/bp990109e.

    Article  CAS  Google Scholar 

  17. Daniel, H. J., Otto, R. T., Reuss, M., & Syldatk, C. (1998). Biotechnology Letters, 20, 805–807. doi:10.1023/B:BILE.0000015927.29348.1a.

    Article  CAS  Google Scholar 

  18. Solaiman, D. K. Y., Ashby, R. D., Nunez, A., & Foglia, T. A. (2004). Biotechnology Letters, 26, 1241–1245. doi:10.1023/B:BILE.0000036605.80577.30.

    Article  CAS  Google Scholar 

  19. Deshpande, M., & Daniels, L. (1995). Bioresource Technology, 54, 143–150. doi:10.1016/0960-8524(95)00116-6.

    Article  CAS  Google Scholar 

  20. Felse, P. A., Shah, V., Chan, J., Rao, K. J., & Gross, R. A. (2007). Enzyme and Microbial Technology, 40, 316–323. doi:10.1016/j.enzmictec.2006.04.013.

    Article  CAS  Google Scholar 

  21. Shah, V., Jurjevic, M., & Badia, D. (2007). Biotechnology Progress, 23, 512–515. doi:10.1021/bp0602909.

    Article  CAS  Google Scholar 

  22. Lazaridou, A., Roukas, T., Biliaderis, C. G., & Vaikousi, H. (2002). Enzyme and Microbial Technology, 31, 122–132. doi:10.1016/S0141-0229(02)00082-0.

    Article  CAS  Google Scholar 

  23. Agarwal, A., Isar, J., Dutt, K., & Saxena, R. K. (2007). Applied Biochemistry and Biotechnology, 42(2), 158–167. doi:10.1007/s12010-007-0014-7.

    Article  CAS  Google Scholar 

  24. Abdel-Mawgoud, A. M., Aboulwafa, M. M., & Hassouna, N. A-H. (2008). Applied Biochemistry and Biotechnology, in press. doi:10.1007/s12010-008-8155-x

  25. Prescott, S., & Dunn’s, A. (1987), in Industrial Microbiology, fourth ed. CBS Publishers and Distributors, New Dehli, India. pp. 710–715.

  26. Rosa, C., & Lachance, M. (1998). International Journal of Systematic Bacteriology, 48, 1413–1417.

    Article  Google Scholar 

  27. Scott Jr, T. A., & Melvin, E. H. (1953). Analytical Chemistry, 25, 1656–1661. doi:10.1021/ac60083a023.

    Article  CAS  Google Scholar 

  28. Hu, Y. H., & Ju, L.-K. (2001). Enzyme and Microbial Technology, 29, 593–601. doi:10.1016/S0141-0229(01)00439-2.

    Article  CAS  Google Scholar 

  29. Nunez, A., Ashby, R., Foglia, T. A., & Solaiman, D. K. Y. (2001). Chromatographia, 53, 673–677. doi:10.1007/BF02493019.

    Article  CAS  Google Scholar 

  30. Cirigliano, M. C., & Carman, G. M. (1984). Applied and Environmental Microbiology, 46, 747–750.

    Google Scholar 

  31. Cirigliano, M. C., & Carman, G. M. (1985). Applied and Environmental Microbiology, 50, 847–850.

    Google Scholar 

  32. Kim, S. Y., Oh, D. K., Lee, K. H., & Kim, J. H. (1997). Applied Microbiology and Biotechnology, 48, 23–26. doi:10.1007/s002530051009.

    Article  CAS  Google Scholar 

  33. Solaiman, D. K. Y., Ashby, R. D., Zerkowski, J. A., & Foglia, T. A. (2007). Biotechnology Letters, 29, 1341–1347. doi:10.1007/s10529-007-9407-5.

    Article  CAS  Google Scholar 

  34. Yongmei, H., & Lu-Kwang, J. (2001). Journal of Biotechnology, 87, 263–272. doi:10.1016/S0168-1656(01)00248-6.

    Article  Google Scholar 

  35. Kim, H. S., Kim, Y. B., Lee, B. S., & Kim, E. K. (2005). Journal of Microbiology and Biotechnology, 15, 55–58.

    CAS  Google Scholar 

  36. Otto, R. T., Daniel, H.-J., Pekin, G., Muller-Decker, K., Furstenberger, G., Reuss, M., et al. (1999). Applied Microbiology and Biotechnology, 52, 495–501. doi:10.1007/s002530051551.

    Article  CAS  Google Scholar 

  37. Cooper, D. G., & Paddock, D. A. (1984). Applied and Environmental Microbiology, 47, 173–176.

    CAS  Google Scholar 

  38. Muthuswami, K., Gopalkrishnan, S., Ravi, T. K., & Sivachidambaram, P. (2008). Current Science, 94(6), 736–747.

    Google Scholar 

  39. Mahanty, B., Pakshirajan, K., & Dasu, V. V. (2006). Applied Biochemistry and Biotechnology, 134, 129–141. doi:10.1385/ABAB:134:2:129.

    Article  CAS  Google Scholar 

  40. Lee, S.-C., Lee, S.-J., Kim, S.-H., Park, I.-H., Lee, Y.-S., Chung, S.-Y., et al. (2008). Bioresource Technology, 99, 2288–2292. doi:10.1016/j.biortech.2007.05.020.

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the research funds from the Department of Science and Technology (DST), India. The authors wish to thank Dr. T. Punniyamurthy, for his help in the analyses of LC-MS and NMR spectra.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Achlesh Daverey & Kannan Pakshirajan

Authors
  1. Achlesh Daverey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kannan Pakshirajan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kannan Pakshirajan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Daverey, A., Pakshirajan, K. Production, Characterization, and Properties of Sophorolipids from the Yeast Candida bombicola using a Low-cost Fermentative Medium. Appl Biochem Biotechnol 158, 663–674 (2009). https://doi.org/10.1007/s12010-008-8449-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-008-8449-z

Keywords

Search

Navigation