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Cellulase Production by Aspergillus japonicus URM5620 Using Waste from Castor Bean (Ricinus communis L.) Under Solid-State Fermentation

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Abstract

The activity of β-glucosidase (βG), total cellulase (FPase) and endoglucanase (CMCase), produced by Aspergillus japonicus URM5620, was studied on solid-state fermentation using castor bean meal as substrate. The effect of the substrate amount, initial moisture, pH, and temperature on cellulase production was studied using a full factorial design (24). The maximum βG, FPase, and CMCase activity was 88.3, 953.4, and 191.6 U/g dry substrate, respectively. The best enzyme activities for all three enzymes were obtained at the same conditions with 5.0 g of substrate, initial moisture 15% at 25 °C and pH 6.0 with 120 h of fermentation. The optimum activity for FPase and CMCase was found at pH 3.0 at an optimum temperature of 50 °C for FPase and of 55 °C for CMCase. The cellulases were stable in the range of pH 3.0–10.0 at 50 °C temperature. The enzyme production optimization demonstrated clearly the impact of the process parameters on the yield of the cellulolytic enzymes.

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Acknowledgments

The authors acknowledge FINEP ref. 2083/07—RENNEBRA/CNPq process no. 552410/2005-5, RENORBIO/MCT/CNPq process no. 554740/2006-0, and CAPES for providing financial support to this research work. The authors also gratefully acknowledge the Nogueira, E.B.S. and the Micoteca URM, of the Mycology Department at UFPE, Brazil.

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Authors and Affiliations

  1. Mycology Department, Federal University of Pernambuco, Cidade Universitária, CEP 50670-420, Recife, Pernambuco, Brazil

    Polyanna Nunes Herculano & Cristina Maria Souza-Motta

  2. Animal Morphology and Physiology Department, Federal Rural University of Pernambuco, Dois Irmãos, CEP 52171-900, Recife, Pernambuco, Brazil

    Polyanna Nunes Herculano, Tatiana Souza Porto, Keila Aparecida Moreira & Ana Lúcia F. Porto

  3. Academic Unit of Garanhuns, Federal Rural University of Pernambuco, CEP 55292-901, Garanhuns, Pernambuco, Brazil

    Tatiana Souza Porto & Keila Aparecida Moreira

  4. Brazilian Agricultural Research Corporation, EMBRAPA/Agribusiness Tropical, CEP 60511-110, Fortaleza, Ceará, Brazil

    Gustavo A. S. Pinto

Authors
  1. Polyanna Nunes Herculano
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  2. Tatiana Souza Porto
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  3. Keila Aparecida Moreira
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  5. Cristina Maria Souza-Motta
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  6. Ana Lúcia F. Porto
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Correspondence to Ana Lúcia F. Porto.

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Herculano, P.N., Porto, T.S., Moreira, K.A. et al. Cellulase Production by Aspergillus japonicus URM5620 Using Waste from Castor Bean (Ricinus communis L.) Under Solid-State Fermentation. Appl Biochem Biotechnol 165, 1057–1067 (2011). https://doi.org/10.1007/s12010-011-9321-0

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