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Efficient Sugarcane Transformation via bar Gene Selection

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Abstract

Genetic engineering provides new opportunities for improving economically important traits in sugarcane cultivars. In this study, an efficient Agrobacterium-mediated transformation system that uses the bar gene (a herbicide resistance gene that is used in conjunction with the herbicide Basta) as a selection marker was developed. Using this transformation selection system, all of the resistant plants after selection were nearly 100% polymerase chain reaction (PCR) detection positive and showed herbicide resistance. Each gram of sugarcane calli used for transformation produced approximately 12 transgenic lines. It took approximately 4 months to generate transgenic plants that measured 10 cm in height for greenhouse transplantation.

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Abbreviations

MS:

Murashige and Skoog medium

GFP:

Green fluorescent protein

2,4-D:

2,4-Dichlorophenoxyacetic acid

6-BA:

6-Benzylaminopurine

NAA:

1-Naphthaleneacetic acid

AD2:

Synthetic Medicago truncatula Defensins4

AD3:

Native Medicago truncatula Defensins4 with KDEL

AD4:

Nynthetic Medicago truncatula Defensins4 with KDEL

Ppase:

Inorganic Pyrophosphatase Gene

SST:

Sucrose:sucrose 1-fructosyl transferase gene

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Acknowledgements

Mr. Wenzhi Wang received financial support from Hainan Natural Science Foundation of Hainan Province of China (20163122). Ms. Shuzhen Zhang received financial support from National Natural Science Foundation of China (31371687).

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Authors and Affiliations

  1. Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences, Xueyuan Rd, Haikou, Hainan, 571101, China

    W. Z. Wang, B. P. Yang, C. L. Feng, J. G. Wang, G. R. Xiong, T. T. Zhao & S. Z. Zhang

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  1. W. Z. Wang
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  2. B. P. Yang
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  3. C. L. Feng
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  4. J. G. Wang
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  5. G. R. Xiong
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  6. T. T. Zhao
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  7. S. Z. Zhang
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Correspondence to S. Z. Zhang.

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Communicated by: Hugo A. Campos

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Wang, W.Z., Yang, B.P., Feng, C.L. et al. Efficient Sugarcane Transformation via bar Gene Selection. Tropical Plant Biol. 10, 77–85 (2017). https://doi.org/10.1007/s12042-017-9186-7

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  • DOI: https://doi.org/10.1007/s12042-017-9186-7

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