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A Highly Sensitive Aptasensor for Sulfamethazine Detection Using an Enzyme-Linked Aptamer Assay

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Abstract

To detect sulfamethazine (SMZ) residues in edible animal products, a colorimetric aptasensor that is based on indirect competitive enzyme-linked aptamer assay (ELAA) is developed in this study. This ELAA uses SMZ-specific aptamers as recognition elements and enzyme as the signal readout element. The detection sensitivity and the specificity of the ELAA were investigated. Our results showed that the indirect competitive ELAA had a ultrasensitivity with a low detection limit of 0.05 ng mL−1. In addition, the proposed indirect competitive ELAA exhibited an 50% inhibition concentration value of 0.64 ng mL−1 for SMZ, and recoveries up to 80.5–92.3% with coefficients of variation of less than 10%. Furthermore, when the ELAA was used for incurred samples by detecting SMZ in chicken muscles, the results obtained by the ELAA methods showed a good agreement with those obtained by established TRFIA and HPLC methods. These results demonstrate that the proposed indirect competitive ELAA can be efficiently used with good accuracy and precision, providing a simple method for the detection of SMZ residue in animal tissues.

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References

  • Chen R, Li H, Zhang H, Zhang S, Shi W, Shen J, Wang Z (2013) Development of a lateral flow fluorescent microsphere immunoassay for the determination of sulfamethazine in milk. Anal Bioanal Chem 45:6783–6789

    Article  CAS  Google Scholar 

  • Chen Y, Guo L, Liu L, Song S, Kuang H, Xu C (2017) Ultrasensitive immunochromatographic strip for fast screening of 27 sulfonamides in honey and pork liver samples based on a monoclonal antibody. J Agric Food Chem 65:8248–8255

    Article  CAS  PubMed  Google Scholar 

  • Clark S, Turnipseed S, Madson M, Hurlbut J, Kuck L, Sofos J (2005) Confirmation of sulfamethazine, sulfathiazole, and sulfadimethoxine residues in condensed milk and soft-cheese products by liquid chromatography/tandem mass spectrometry. J AOAC Int 88:736–743

    CAS  PubMed  Google Scholar 

  • Cratacos-Cubarsi M, Castellari M, Garcia-Regueiro J (2006) Detection of sulphamethazine residues in cattle and pig hair by HPLC-DAD. J Chromatogr B 832:121–126

    Article  CAS  Google Scholar 

  • Ding S, Chen J, Jiang H, He J, Shi W, Zhao W, Shen J (2006) Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue. J Agric Food Chem 54:6139–6142

    Article  CAS  PubMed  Google Scholar 

  • Font G, Juan-Garcia A, Pico Y (2007) Pressurized liquid extraction combined with capillary electrophoresis-mass spectrometry as an improved methodology for the determination of sulfonamide residues in meat. J Chromatogr A 1159:233–241

    Article  CAS  PubMed  Google Scholar 

  • Furusawa N (2003) A clean and rapid liquid chromatographic technique for sulfamethazine monitoring in pork tissues without using organic solvents. J Chromatogr Sci 44:377–380

    Article  Google Scholar 

  • Guo Y, Ngom B, Le T, Jin X, Wang L, Shi D, Wang X, Bi D (2010) Utilizing three monoclonal antibodies in the development of an immunochromatographic assay for simultaneous detection of sulfamethazine, sulfadiazine, and sulfaquinoxaline residues in egg and chicken muscle. Anal Chem 82:7550–7555

    Article  CAS  PubMed  Google Scholar 

  • He J, Shen J, Suo X, Jiang H, Hou X (2005) Development of a monoclonal antibody-based ELISA for detection of sulfamethazine and N4-acetyl sulfamethazine in chicken breast muscle tissue. J Food Sci 70:C113–C117

    Article  CAS  Google Scholar 

  • He J, Wang S, Fang G, Zhu H, Zhang Y (2008) Molecularly imprinted polymer online solid-phase extraction coupled with high-performance liquid chromatography−UV for the determination of three sulfonamides in pork and chicken. J Agric Food Chem 56:2919–2925

    Article  CAS  PubMed  Google Scholar 

  • Le T, Yan P, Liu J, Wei S (2013) Simultaneous detection of sulfamethazine and sulfaquinoxaline using a dual-label time-resolved fluorescence immunoassay. Food Addit Contam A 30:1264–1269

    Article  CAS  Google Scholar 

  • Lee HJ, Lee MH, Han IK (2001) Application of ELISA for the detection of sulfamethazine residue in live cattle. Asian-Australas J Anim Sci 14:378–381

    Article  CAS  Google Scholar 

  • Li X, Zhang G, Liu Q, Feng C, Wang X, Yang Y, Xiao Z, Yang J, Xing G, Zhao D, Cai S, Chen H (2009) Development of immunoassays for the detection of sulfamethazine in swine urine. Food Addit Contam A 26:314–325

    Article  CAS  Google Scholar 

  • Liu X, Gao T, Gao X, Ma T, Tang Y, Zhu L, Li J (2017) An aptamer based sulfadimethoxine assay that uses magnetized upconversion nanoparticles. Microchim Acta 184:3557–3563

    Article  CAS  Google Scholar 

  • Lv Z, Chen A, Liu J, Guan Z, Zhou Y, Xu S, Yang S, Li C (2014) A simple and sensitive approach for ochratoxin a detection using a label-free fluorescent aptasensor. PLoS One 9:e85968. https://doi.org/10.1371/journal.pone.0085968

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ma M, Wen K, Beier RC, Eremin SA, Li C, Zhang S, Shen J, Wang Z (2016) Chemiluminescence resonance energy transfer competitive immunoassay employing hapten-functionalized quantum dots for the detection of sulfamethazine. ACS Appl Mater Interfaces 8:17745–17750

    Article  CAS  PubMed  Google Scholar 

  • Mohammad DN, Ramezani M, Sarreshtehdar EA, Abnous K, Taghdisi SM (2016) A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin. Biosens Bioelectron 75:123–128

    Article  CAS  Google Scholar 

  • Spielmeyer A, Ahlborn J, Hamscher G (2014) Simultaneous determination of 14 sulfonamides and tetracyclines in biogas plants by liquid-liquid-extraction and liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 40:2513–2524

    Article  CAS  Google Scholar 

  • Sun Y, Xu J, Li W, Cao B, Wang DD, Yang Y, Lin QX, Li JL, Zheng TS (2014) Simultaneous detection of ochratoxin a and fumonisin B1 in cereal samples using an aptamer−photonic crystal encoded suspension array. Anal Chem 86:11797–11802

    Article  CAS  Google Scholar 

  • Wang X, Li K, Shi D, Xiong N, Jin X, Yi J, Bi D (2007) Development of an immunochromatographic lateral-flow test strip for rapid detection of sulfonamides in eggs and chicken muscles. J Agric Food Chem 55:2072–2078

    Article  CAS  PubMed  Google Scholar 

  • Wang S, Yong W, Liu J, Zhang L, Chen Q, Dong Y (2014) Development of an indirect competitive assay-based aptasensor for highly sensitive detection of tetracycline residue in honey. Biosens Bioelectron 57:192–198

    Article  CAS  PubMed  Google Scholar 

  • Wu S, Duan N, Wang Z, Wang H (2011) Aptamer-functionalized magnetic nanoparticle-based bioassay for the detection of ochratoxin a using upconversion nanoparticles as labels. Analyst 136:2306–2314

    Article  CAS  PubMed  Google Scholar 

  • Xiao C, Chen F, Liu Z, Zhang Y, Chen C, Chen H, Zhou H, Gao Z (2012) Ultrasound-assisted extraction combined with HPLC-UV for fast determination of sulfamethazine and its n4-acetyl metabolite in plasma and phosphate buffer. Anal Lett 45:1836–1848

    Article  CAS  Google Scholar 

  • Xu ZL, Shen YD, Sun YM, Campbell K, Tian YX, Zhang SW, Lei HT, Jiang YM (2013) Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). Talanta 103:306–313

    Article  CAS  PubMed  Google Scholar 

  • Yan X, Cao Z, Lau C, Lu J (2010) DNA aptamer folding on magnetic beads for sequential detection of adenosine and cocaine by substrate-resolved chemiluminescence technology. Analyst 135:2400–2407

    Article  CAS  PubMed  Google Scholar 

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Funding

The work was supported by the National Natural Science Foundation of China (Grant No. 31671939), China Scholarship Council (CSC No. 201608505051), and Chongqing Normal University.

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Authors and Affiliations

  1. College of Life Science, Chongqing Normal University, 37 Chengzhong Road, University Town, Chongqing, 401331, People’s Republic of China

    Tao Le, Qi Sun, Yong Xie, Lihui Shu, Jin Liu, Jian Xu & Jin Xiong

  2. Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur, Ottawa, ON, K1N 6N5, Canada

    Xudong Cao

Authors
  1. Tao Le
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  2. Qi Sun
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  3. Yong Xie
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  4. Lihui Shu
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  5. Jin Liu
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  6. Jian Xu
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  7. Jin Xiong
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  8. Xudong Cao
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Corresponding authors

Correspondence to Tao Le or Xudong Cao.

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Conflict of Interest

Tao Le declares that he has no conflict of interest. Qi Sun declares that he has no conflict of interest. Yong Xie declares that he has no conflict of interest. Lihui Shu declares that she has no conflict of interest. Jin Liu declares that she has no conflict of interest. Jian Xu declares that he has no conflict of interest. Jin Xiong declares that he has no conflict of interest. Xudong Cao declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human subjects. All animal experiments that were described in the present study were performed in adherence to Chongqing Normal University Animal Experiment Center Guidelines and approved by the Animal Ethics Committee.

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Le, T., Sun, Q., Xie, Y. et al. A Highly Sensitive Aptasensor for Sulfamethazine Detection Using an Enzyme-Linked Aptamer Assay. Food Anal. Methods 11, 2778–2787 (2018). https://doi.org/10.1007/s12161-018-1258-2

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  • DOI: https://doi.org/10.1007/s12161-018-1258-2

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