Abstract
A new process for co-extraction and separation of fatty acids and carotenoids from Rhodosporidium toruloides NCYC 921 biomass in order to achieve full exploitation of the yeast lipidic fraction is described. A saponification of the wet yeast biomass was performed using a potassium hydroxide solution (1.1 M) in ethanol 96%, at 65 °C for 180 min. In the carotenoid extraction step, a biphasic system with an organic:aqueous phases ratio of 0.49 mL/mL and a water content of 18.9% (w/w) was used. In the presence of an acid catalyst, the fatty acid fraction was esterified into fatty acids ethyl esters. The yeast biomass downstream processing allowed reaching a fatty acid and total carotenoids recovery yields of 91.0% and 85.2%, respectively. The process reported here takes advantage of various components of the yeast biomass, therefore maximizing the value derived from the biomass feedstock, with a minimal environmental impact within the frame of circular bioeconomy.
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Acknowledgements
The present work was carried out within the project FCOMP-01-0124-FEDER-019317 ex-PTDC/AAC-AMB/116594/2010 entitled “CAROFUEL – New process for a sustainable microbial biodiesel production: The yeast Rhodotorula glutinis biorefinery as a source of biodiesel, biogas and carotenoids” supported by FCT (Fundação para a Ciência e a Tecnologia-Portugal) (also supported by FEDER funding through COMPETE – Programa Operacional Factores de Competitividade). The authors acknowledge Natércia Santos, Carlos Barata and Margarida Monteiro for laboratory assistance.
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Passarinho, P.C., Oliveira, B., Dias, C. et al. Sequential Carotenoids Extraction and Biodiesel Production from Rhodosporidium toruloides NCYC 921 Biomass. Waste Biomass Valor 11, 2075–2086 (2020). https://doi.org/10.1007/s12649-018-0489-1
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DOI: https://doi.org/10.1007/s12649-018-0489-1