Abstract
The cellulase and xylanase productions from the pretreated empty fruit bunch as the low-cost substrate in a submerged fermentation were investigated. The objectives of this study were selected the local strain that produced high cellulase and xylanase and enhanced the enzymes production by the selected strain. Ten Streptomyces strains were cultivated in the complex medium [(g/l); CaCl2·2H2O 0.1, MgSO4·7H2O 0.1, KH2PO4 0.5, K2HPO4 1.0, NaCl 0.2, NH4NO3 1.0, yeast extract 5.0 and Tween 80 0.5 with alkaline peroxide pretreated empty fruit bunch (APEFB) 20 in distilled water, pH 7.0] in an incubator shaker at 150 rpm and 45 °C for 120 h. The strain TC13W gave the highest cellulase and xylanase activities (280 and 878 U/g APEFB, respectively). This strain was identified by 16S rDNA method as Streptomyces thermocoprophilus (96% similarity). Cellulase and xylanase productions by S. thermocoprophilus TC13W in the optimized medium with 1% (w/v) APEFB and 0.5% (w/v) yeast extract, pH 6.5 at 150 rpm and 40 °C for 120 h gave the maximum cellulase and xylanase activities of 925 and 1796 U/g APEFB, respectively. The increasing of cellulase and xylanase activities in the optimized medium was 3.30 and 2.04 folds, respectively in comparison to the original medium. These in-house enzymes could be used as the promising candidate enzyme for produce various value-added products via an enzymatic hydrolysis of the lignocellulosic waste.
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References
Prasertsan, S., Prasertsan, P.: Biomass residues from palm oil mills in Thailand: an overview on quantity and potential usage. Biomass Bioenergy 11, 387–395 (1996)
Ariffin, H., Hassan, M.A., Shah, U.K., Abdullah, N., Ghazali, F.M., Shirai, Y.: Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by Bacillus pumilus EB3. J. Biosci. Bioeng. 106, 231–236 (2008)
Harun, N.A.F., Baharuddin, A.S., Zainudin, M.H.M., Bahrin, E.K., Naim, M.N., Zakaria, R.: Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca. BioResources 8, 676–687 (2012)
Baldrian, P., Valásková, V.: Degradation of cellulose by basidiomycetous fungi. FEMS Microbiol. Rev. 32, 501–521 (2008)
Bayer, E.A., Chanzy, H., Lamed, R., Shoham, Y.: Cellulose, cellulases and cellulosomes. Curr. Opin. Struct. Biol. 8, 548–557 (1998)
Fontes, C.M.G.A., Gilbert, H.J.: Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu. Rev. Biochem. 79, 655–681 (2010)
Lynd, L.R.: Overview and evaluation of fuel ethanol from cellulosic biomass: technology, economics, the environment, and policy. Annu. Rev. Energy Environ. 21, 403–465 (1996)
Adsul, M.G., Bastawde, K.B., Varma, A.J., Gokhale, D.V.: Strain improvement of Penicillium janthinellum NCIM 1171 for increased cellulase production. Bioresour. Technol. 98, 1467–1473 (2007)
Camassola, M., Dillon, A.J.P.: Biological pretreatment of sugar cane bagasse for the production of cellulases and xylanases by Penicillium echinulatum. Ind. Crops Prod. 29, 642–647 (2009)
Katapodis, P., Christakopoulou, V., Kekos, D., Christakopoulos, P.: Optimization of xylanase production by Chaetomium thermophilum in wheat straw using response surface methodology. Biochem. Eng. J. 35, 136–141 (2007)
Yang, C., Shen, Z., Yu, G., Wang, J.: Effect and aftereffect of γ radiation pretreatment on enzymatic hydrolysis of wheat straw. Bioresour. Technol. 99, 6240–6245 (2008)
Ang, S.K., Shaza, E.M., Adibah, Y., Suraini, A.A., Madihah, M.S.: Production of cellulase and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation. Process Biochem. 48, 1293–1302 (2013)
Umikalsom, M.S., Ariff, A.B., Shamsuddin, Z.H., Tong, C.C., Hassan, M.A., Karim, M.I.A.: Production of cellulase by a wild strain of Chaetomium globosumusing delignified oil palm empty-fruit-bunch fibre as substrate. Appl. Microbiol. Biotechnol. 47, 590–595 (1997)
Alam, M.Z., Mamun, A.A., Qudsieh, I.Y., Muyibi, S.A., Salleh, H.M., Omar, N.M.: Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor. Biochem. Eng. J. 46, 61–64 (2009)
Ahamed, A., Vermette, P.: Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions. Biochem. Eng. J. 40, 399–407 (2008)
Archana, A., Satyanarayana, T.: Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation. Enzyme Microb. Technol. 21, 12–17 (1997)
Bhushan, B., Pal, A., Jain, V.: Isolation, screening and optimized production of extracellular xylanase under submerged condition from Aspergillus flavus Mtcc 9390. Enzyme Eng. 1, 1–6 (2012)
Elegir, G., Szakács, G., Jeffries, T.W.: Purification, characterization, and substrate specificities of multiple xylanases from Streptomyces sp. strain B-12-2. Appl. Environ. Microbiol. 60, 2609–2615 (1994)
Kim, S., Kim, C.H.: Production of cellulase enzymes during the solid-state fermentation of empty palm fruit bunch fiber. Bioprocess Biosyst. Eng. 35, 61–67 (2012)
Ting, A.S.Y., Hermanto, A., Peh, K.L.: Indigenous actinomycetes from empty fruit bunch compost of oil palm: evaluation on enzymatic and antagonistic properties. Biocatal. Agric. Biotechnol. 3, 310–315 (2014)
Gilbert, M., Morosoli, R., Shareck, F., Kluepfel, D.: Production and secretion of proteins by Streptomycetes. Crit. Rev. Biotechnol. 15, 13–39 (1995)
Vasavada, S.H., Thumar, J.T., Singh, S.P.: Secretion of a potent antibiotic by salt-tolerant and alkaliphilic actinomycete Streptomyces sannanensis strain RJT-1. Curr. Sci. 91, 1393–1397 (2006)
Kamcharoen, A., Champreda, V., Eurwilaichitr, L., Boonsawang, P.: Screening and optimization of parameters affecting fungal pretreatment of oil palm empty fruit bunch (EFB) by experimental design. Int. J. Energy Environ. Eng. 5, 303–312 (2014)
A.O.A.C.: Official Methods of Analysis of the Association of Official Analytical Chemists, 15th edn. A.O.A.C., Washington, D.C. (1990)
Jang, H.-D., Chang, K.-S.: Thermostable cellulases from Streptomycessp.: scale-up production in a 50-l fermenter. Biotechnol. Lett. 27, 239–242 (2005)
Miller, G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426–428 (1959)
Baharuddin, A.S., Razak, N.A.A., Rahman, N.A.A., Budiatman, S., Shirai, Y., Hassan, M.A.: Bioconversion of oil palm empty fruit bunch by Aspergillus niger EB4 under solid-state fermentation. Pertanika J. Trop. Agric. Sci. 32, 143–151 (2009)
Cuesta, G., García-de-la-Fuente, R., Abad, M., Fornes, F.: Isolation and identification of actinomycetes from a compost-amended soil with potential as biocontrol agents. J. Environ. Manag. 95(Suppl), 280–284 (2012)
Kang, S.W., Park, Y.S., Lee, J.S., Hong, S.I., Kim, S.W.: Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass. Bioresour. Technol. 91, 153–156 (2004)
Rateb, M.E., Houssen, W.E., Harrison, W.T.A., Deng, H., Okoro, C.K., Asenjo, J.A., Andrews, B.A., Bull, A.T., Goodfellow, M., Ebel, R., Jaspars, M.: Diverse metabolic profiles of a Streptomyces strain isolated from a hyper-arid environment. J. Nat. Prod. 74, 1965–1971 (2011)
Semêdo, L.T.A.S., Gomes, R.C., Bon, E.P.S., Soares, R.M.A., Linhares, L.F., Coelho, R.R.R.: Endocellulase and exocellulase activities of two Streptomyces strains isolated from a forest soil. Appl. Biochem. Biotechnol. 84–86, 267–276 (2000)
Jang, H.D., Chen, K.S.: Production and characterization of thermostable cellulases from Streptomyces transformant T3-1. World J. Microbiol. Biotechnol. 19, 263–268 (2003)
Grigorevski de Lima, A.L., Pires do Nascimento, R., da Silva Bon, E.P., Coelho, R.R.R.: Streptomyces drozdowiczii cellulase production using agro-industrial by-products and its potential use in the detergent and textile industries. Enzyme Microb. Technol. 37, 272–277 (2005)
Chellapandi, P., Jani, H.M.: Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation. Braz. J. Microbiol. 39, 122–127 (2008)
Prasad, P., Bedi, S., Singh, T.: In vitro cellulose rich organic material degradation by cellulolytic Streptomyces albospinus (MTCC 8768). Malays. J. Microbiol. 8, 164–169 (2012)
Saratale, G.D., Saratale, R.G., Oh, S.E.: Production and characterization of multiple cellulolytic enzymes by isolated Streptomyces sp. MDS. Biomass Bioenergy 47, 302–315 (2012)
Bahrin, E., Ibrahim, M., Abd Razak, M.N., Abd-Aziz, S., Md Shah, U.K., Alitheen, N., Salleh, M.: Improved cellulase production by Botryosphaeria rhodina from OPEFB at low level moisture condition through statistical optimization. Prep. Biochem. Biotechnol. 42, 155–170 (2012)
Shahriarinour, M., Wahab, M.N.A., Mohamad, R., Mustafa, S., Ariff, A.B.: Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus. Afr. J. Biotechnol. 10, 7459–7467 (2011)
Bakri, Y., Jawhar, M., Arabi, M.: Improvement of xylanase production by Cochliobolus sativus in solid state fermentation. Braz. J. Microbiol. 39, 602–604 (2008)
Goyal, V., Mittal, A., Bhuwal, A.K., Singh, G., Yadav, A., Aggarwal, N.K.: Parametric optimization of cultural conditions for carboxymethyl cellulase production using pretreated rice straw by Bacillus sp. 313SI under stationary and shaking conditions. Biotechnol. Res. Int. 2014, 1–7 (2014)
Fatokun, E.N., Nwodo, U.U., Okoh, A.I.: Classical optimization of cellulase and xylanase production by a marine Streptomyces species. Appl. Sci. 6, 1–14 (2016)
Macedo, E.P., Cerqueira, C.L.O., Souza, D.A.J., Bispo, A.S.R., Coelho, R.R.R., Nascimento, R.P.: Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08. Braz. J. Chem. Eng. 30, 729–735 (2013)
Nascimento, R.P., Coelho, R.R.R., Marques, S., Alves, L., Gı́rio, F.M., Bon, E.P.S., Amaral-Collaço, M.T.: Production and partial characterisation of xylanase from Streptomyces sp. strain AMT-3 isolated from Brazilian cerrado soil. Enzyme Microb. Technol. 31, 549–555 (2002)
Tuncer, M., Kuru, A., Isikli, M., Sahin, N., Celenk, F.G.: Optimization of extracellular endoxylanase, endoglucanase and peroxidase production by Streptomyces sp. F2621 isolated in Turkey. J. Appl. Microbiol. 97, 783–791 (2004)
Jaradat, Z., Dawagreh, A., Ababneh, Q., Saadoun, I.: Influence of culture conditions on cellulase production by Streptomyces sp. (strain J2). Int. J. Life Sci. Med. Res. 3, 141–146 (2008)
Narasimha, G., Sridevi, A., Buddolla, V., Subhosh, C.M., Rajasekhar, R.B.: Nutrient effects on production of cellulolytic enzymes by Aspergillus niger. Afr. J. Biotechnol. 5, 472–476 (2006)
Chaiyaso, T., Kuntiya, A., Techapun, C., Leksawasdi, N., Seesuriyachan, P., Hanmoungjai, P.: Optimization of cellulase-free xylanase production by thermophilic Streptomyces thermovulgaris TISTR1948 through Plackett-Burman and response surface methodological approaches. Biosci. Biotechnol. Biochem. 75, 531–537 (2011)
Pandey, A.: Effect of particle size of substrate of enzyme production in solid-state fermentation. Bioresour. Technol. 37, 169–172 (1991)
Acknowledgements
The authors would like to thank the Office of the Higher Education Commission (Strategic Scholarships of Frontier Research Network, specific for the Southern region), the National Research Council of Thailand and the Graduate School of Prince of Songkla University for supporting this study.
Funding
This study was funded by Strategic Scholarships of Frontier Research Network (Specific for the Southern region), the Office of the Higher Education Commission (Grant No. 022/2012), National Research Council of Thailand (Grant year 2014), and the Graduate School of Prince of Songkla University.
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Sinjaroonsak, S., Chaiyaso, T. & H-Kittikun, A. Optimization of Cellulase and Xylanase Productions by Streptomyces thermocoprophilus TC13W Using Low Cost Pretreated Oil Palm Empty Fruit Bunch. Waste Biomass Valor 11, 3925–3936 (2020). https://doi.org/10.1007/s12649-019-00720-y
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DOI: https://doi.org/10.1007/s12649-019-00720-y